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Effect of ws6 on actin formation in trabecular meshwork cells

A technology of actin and trabecular meshwork, applied in the medical field, can solve problems such as high intraocular pressure and achieve the effect of reducing intraocular pressure

Active Publication Date: 2020-10-30
QINGDAO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The resistance to aqueous humor efflux mainly comes from the trabecular meshwork efflux pathway, and the aqueous humor efflux pathway is usually abnormal in patients with primary open-angle glaucoma, resulting in high intraocular pressure

Method used

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  • Effect of ws6 on actin formation in trabecular meshwork cells
  • Effect of ws6 on actin formation in trabecular meshwork cells

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Experimental program
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Embodiment Construction

[0013] The present invention will be described in detail below in conjunction with specific embodiments.

[0014] Cytotoxicity of 1WS6 to trabecular meshwork cells

[0015] First, we selected 4 gradient concentrations of WS6 at 10μM, 100μM, 200μM, 300μM, etc. for its toxicity test on trabecular meshwork cells. The results showed that after 24h, with the increase of WS6 concentration, the death rate of trabecular meshwork cells gradually increased, reaching about 50% at 200μM. Therefore, we first selected 10μM WS6 for the pharmacodynamic test.

[0016] 2WS6 inhibits the formation of actin in trabecular meshwork cells

[0017] We studied the inhibitory effect of WS6 on the formation of actin in trabecular meshwork cells through the specific fluorescent staining technique of phalloidin and the laser confocal technique. Such as figure 1 , figure 2 The results shown show that the TRPV4 agonist GSK101 can significantly enhance the fluorescence intensity of trabecular meshwork cell agoni...

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Abstract

The invention discloses influence of WS6 on actin formation of trabecular meshwork cells. WS6 refers to anti-diabetic medicine glibenclamide, sodium orthovanadate and an active compound capable of promoting pancreatic beta cell proliferation. WS6 has cytotoxicity on the trabecular meshwork cells and inhibits actin formation of the trabecular meshwork cells. The influence of WS6 has the beneficialeffect that the fact that WS6 can remarkably inhibit actin formation, caused by TRPV4 agonist GSK101, of the trabecular meshwork cells is revealed. Intraocular pressure can be reduced, and the influence of WS6 has potential treatment significance in glaucoma caused by increase of intraocular pressure.

Description

Technical field [0001] The invention belongs to the field of medical technology, and relates to the effect of WS6 (a drug for promoting islet cell proliferation) on the formation of actin in trabecular meshwork cells. Background technique [0002] Glaucoma (Glaucomas) is a kind of optic nerve disease caused by degeneration of retinal ganglion cells, which can cause optic disc depression, visual field defect, and eventually blindness. [0003] Studies have shown that high intraocular pressure is an important treatment factor for glaucoma. Every 1mm Hg reduction in intraocular pressure can reduce the progress of glaucoma by approximately 10%. The high intraocular pressure is mainly caused by the imbalance of the aqueous circulation in the anterior segment. Aqueous humor is produced in the ciliary body, enters the aqueous circulation of the anterior segment of the eye through the narrow gap between the iris and the lens, and then is discharged from the eye through the drainage of aq...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/64A61K33/00A61K45/00A61P27/06G01N21/64
CPCA61K31/64A61K33/00A61K45/00A61P27/06G01N21/6486
Inventor 张从晓王克威朱玮于红霞
Owner QINGDAO UNIV
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