Polypeptide composition with special immune function, vaccine and application thereof
A technology of polypeptide composition and immune function, which is applied in the field of biomedicine and can solve problems that are not suitable for large-scale individuals
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Embodiment 1
[0017] Example 1: Polypeptide composition combined with different Toll-like receptor ligands, or combined with aIL10R immunization
[0018] Experimental procedure:
[0019] C57BL / 6 mice were randomly divided into 4 groups, group 1 was EX+MPLA; group 2 was EX+MPLA+Rat IgG; group 3 was EX+MPLA+aIL10R; group 4 was PBS control group. The four groups of mice were subcutaneously immunized on the left abdomen, once a week, and immunized twice in total. The mice were sacrificed on the 6th day after the last immunization, and the inguinal draining lymph nodes were removed and photographed. The results showed that: 1. When EX+MPLA was used for immunization, the draining lymph nodes were significantly larger than that of the PBS control group, which indicated that the combination of EX and different Toll receptor ligands could enhance the immune response of the draining lymph nodes; 2. Using EX+MPLA+ When immunized with aIL10R, the draining lymph nodes were larger than those of the exp...
Embodiment 2
[0021] Example 2: Polypeptide composition combined with different Toll-like receptor ligands, or combined with aIL10R immunization
[0022] Experimental procedure:
[0023] C57BL / 6 mice were randomly divided into 4 groups, group 1 was EX+MPLA; group 2 was EX+MPLA+Rat IgG; group 3 was EX+MPLA+aIL10R; group 4 was PBS control group. The mice were subcutaneously immunized on the left side of the abdomen, once a week, and immunized twice in total. The mice were sacrificed on the 6th day after the last immunization, and the spleen was taken to prepare a single cell suspension, which was stimulated by the E7 epitope (the amino acid sequence of which was RAHYNIVTF), and the antigen-specific IFNγ+CD8+T cells were detected by enzyme-linked dot immunoassay. The results showed that: 1. When EX+MPLA was used for immunization, more specific IFNγ+CD8+T cells could be induced compared with PBS control group; The test group immunized with EX+MPLA+Rat IgG can induce a stronger IFNγ+CD8+ T cel...
Embodiment 3
[0025] Example 3: The combination of polypeptide composition and MPLA, combined with aIL10R immunization, preventive effect on HPV16E6 / E7 transformed mouse TC-1 tumor
[0026] Experimental procedure:
[0027] C57BL / 6 mice were randomly divided into 4 groups, namely EX+MPLA group, EX+MPLA+Rat IgG group, EX+MPLA+aIL10R group and PBS control group. The mice were subcutaneously immunized on the left hind legs, once a week, and immunized twice in total. On the 7th day after the second immunization, 2 × 10 5 A HPV16E6 / E7 transforms TC-1 tumor cells for attack. The tumor size was measured twice a week and the survival time of the mice was observed. Eighty days after immunization, the mice that survived the first TC-1 tumor inoculation challenge received the second TC-1 tumor inoculation challenge, and a new PBS control group was added. The tumor size was measured twice a week and the survival time of the mice was observed. The results show that: 1. On the 7th day after the EX+MP...
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