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A Fluorescent Analysis Method for Measuring Artemisinin Content
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A fluorescence analysis, artemisinin technology, applied in the field of spectral analysis and detection, can solve problems such as high cost and complicated operation
Active Publication Date: 2020-07-07
湘西自治州奥瑞克医药化工有限责任公司
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[0005] The purpose of the present invention is to overcome the problems of complex operation and high cost in the current detection process of artemisinin, and provide a fluorescence analysis method for determining the content of artemisinin based on a new type of nanomaterial graphene quantum dot. The unique properties of graphene quantum dots and the special peroxide bridge structure of artemisinin constitute a simple and sensitive detection system, which realizes the quantitative detection of artemisinin
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Embodiment 1
[0032] Example 1 A fluorescence analysis method for determining the content of artemisinin, specifically as figure 1 As shown, the steps are as follows:
[0033] (1) Add concentration of 0 µM, 4 µM, 10 µM, 40 µM, 60 µM, 100 µM, 140 µM, 180 µM, 200 µM, 600 µM, 1000 µM, 1400 µM, 1800 to 14 centrifuge tubes µM and 2200 µM artemisinin ethanol solution 5 µL; the chemical structure of artemisinin is shown in formula (1);
[0036] (3) Add 20 µL of 1 mg / mL tyrosinase solution to the centrifuge tube solution obtained in step (2), and react in a water bath at 40°C for 10 min;
[0037] (4) Add graphene quantum dots to the centrifuge tube solution obtained in step (3) to make the concentration 0.5 mg / mL, mix well, and react at room temperature...
Embodiment 2
[0039] Example 2 Selective Analysis and Detection Method of Artemisinin
[0042] (3) Add 1 mg / mL tyrosinase solution to the centrifuge tube solution obtained in step (2), and react in a water bath at 40°C for 10 min;
[0043] (4) Add graphene quantum dots to the centrifuge tube solution obtained in step (3) to make the concentration 0.5 mg / mL, mix well, and react for 25 min;
[0044] (5) Add buffer solution to the centrifuge tubes obtained in step (4), so that the volume of the solution in each test tube is 200 µL, and measure the fluorescence intensity values of the centrifuge tube ...
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technical field [0001] The invention belongs to the technical field of spectral analysis and detection, and in particular relates to a fluorescence analysis method using a novel nanomaterial graphene quantum dot, and in particular to a fluorescence analysis method for determining the content of artemisinin. Background technique [0002] Artemisinin is a sesquiterpenelactone compound containing peroxy groups. It is the safest and most effective antimalarial drug after pyrimethamine, chloroquine and primaquine. It has the characteristics of quick action and low toxicity. At the same time, artemisinin also shows good prospects in the treatment of anti-schistosomiasis, mediation or suppression of humoral immune function, and improvement of lymphocyte transformation rate. With the wide application of artemisinin in the field of biochemical medicine, the market demand for artemisinin is expanding rapidly, which provides an opportunity for unscrupulous traders to sell counterfeit ...
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