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A kind of sulfonated peony seed polysaccharide and its application in the preparation of auxiliary medicine for treating liver cancer

A technology for the treatment of liver cancer and peony seeds, which is applied in the field of sulfonated peony seed polysaccharides and preparation of auxiliary drugs for the treatment of liver cancer, can solve the problems of liver and kidney toxicity, and no public literature mentions the anti-liver tumor function of peony seed polysaccharides, etc., and achieves a large Value, inhibition of liver cancer cell proliferation or liver tumor growth function, no toxic side effects

Active Publication Date: 2020-11-24
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Primary liver cancer is one of the most common malignant tumors in the world. At present, surgery and chemotherapy are still the main treatments for liver cancer, but chemotherapy often leads to severe liver and kidney toxicity and other adverse reactions; polysaccharides; at present, it has been found that a variety of plant and fungal polysaccharides have anti-tumor, immune regulation and other functions; All aspects have biological activity that neutral polysaccharides can’t reach; so far, there is no published literature mentioning the anti-hepatic tumor function of tree peony seed polysaccharides, and there is no modification of tree peony seed polysaccharides into sulfonated polysaccharides, and its Preparation of Anti-liver Cancer Drugs Report

Method used

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  • A kind of sulfonated peony seed polysaccharide and its application in the preparation of auxiliary medicine for treating liver cancer
  • A kind of sulfonated peony seed polysaccharide and its application in the preparation of auxiliary medicine for treating liver cancer
  • A kind of sulfonated peony seed polysaccharide and its application in the preparation of auxiliary medicine for treating liver cancer

Examples

Experimental program
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Effect test

Embodiment 1

[0027] The specific operation steps for preparing sulfonated peony seed polysaccharide are as follows:

[0028] (1) Peony seed meal removal

[0029] Take 200 g of peony seed meal after oil extraction, add 6 L of petroleum ether, stir and extract for 30 minutes, filter with suction, discard the filtrate, add 6 L of petroleum ether to the filter residue, stir and extract for 30 minutes, filter with suction, and dry the filter residue in air , Get 153 g of dry and clean peony seed meal.

[0030] (2) Separation of crude polysaccharides from peony seeds. Take 150 g of dry and clean peony seed meal, add 3 L of acetic acid buffer (0.05mol / L sodium acetate, pH=5.2), stir for 30 minutes, filter, the filtrate is deproteinized by Sevage method, dialyzed, concentrated and then freeze-dried to obtain peony Seed crude polysaccharide 17 g.

[0031] (3) Refined peony seed polysaccharide. Take 15 g of peony seed polysaccharide and pass DEAE-cellulose column chromatography and dextran gel column chr...

Embodiment 2

[0057] (1) Remove impurities from peony seed meal. Take 500 g of peony seed meal after oil extraction, add 10 L petroleum ether, stir and extract for 30 minutes, filter with suction, discard the filtrate, add 10 L petroleum ether to the filter residue, stir and extract for 30 minutes, filter with suction, and dry the filter residue in air , Get dry clean peony seed meal 397 g.

[0058] (2) Separation of crude polysaccharides from peony seeds. Take 350 g of dry clean peony seed meal, add 10 L of acetic acid buffer (0.05mol / L sodium acetate, pH=5.2), stir for 30 minutes, filter, the filtrate is deproteinized by Sevage method, dialyzed, concentrated and then freeze-dried to obtain peony Seed crude polysaccharide 44 g.

[0059] (3) Refined peony seed polysaccharide. Take 40 g of peony seed polysaccharide and pass DEAE-cellulose column chromatography and dextran gel column chromatography. The chromatographic solution is concentrated and lyophilized to obtain 8.3 g of peony seed polys...

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Abstract

The invention relates to sulfonated peony seed polysaccharide. The sulfonated peony seed polysaccharide is obtained by carrying out sulfonation modification on peony seed meal polysaccharide for oil use with dilute sulfuric acid and is light yellow powder, the hydroxyl substitution degree exceeds 0.7, and infrared spectrum absorption is detected to have absorption peaks of S=O, C-O-S and O-S-O groups at the positions of 1250 cm<-1>, 830 cm<-1> and 598 cm<-1> correspondingly. The invention further discloses application of the sulfonated peony seed polysaccharide in preparation of ancillary drugs for treating liver cancer. The sulfonated peony seed polysaccharide is dissolved in normal saline to be prepared into a 175 microgram / mL liquid medicine, the in-vitro experiment and model animal experiment results show that a peony seed polysaccharide solution has an obvious inhibiting effect on the growth of liver cancer cells and is free of toxic and side effects and free of damage to normal cells in vitro.

Description

Technical field [0001] The invention belongs to the technical field of peony seed polysaccharides, and specifically relates to a sulfonated peony seed polysaccharide and its use in preparing auxiliary drugs for the treatment of liver cancer. Background technique [0002] Primary liver cancer is one of the most common malignant tumors in the world. At present, surgery and chemotherapy are still the main treatments for liver cancer, but chemotherapy often leads to severe liver and kidney toxicity and other adverse reactions; polysaccharides are a type of polymerization of monosaccharides. Polysaccharides; At present, a variety of plant and fungal polysaccharides have been found to have anti-tumor and immunomodulatory effects; modern pharmacological studies have shown that sulfonic acid polysaccharides containing sulfate groups are more effective in anti-tumor, anti-coagulation, anti-oxidation, anti-virus, etc. All aspects have biological activities that neutral polysaccharides can’...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00
CPCA61K31/715A61P35/00C08B37/0003
Inventor 魏兆军章建国李晓丽胡飞
Owner HEFEI UNIV OF TECH