Small molecule hyaluronic acid purification method

A technology of hyaluronic acid and purification method, applied in the field of small molecule hyaluronic acid purification, can solve the problems of complex reaction conditions, great influence on product quality, low product recovery rate, etc., and achieve the effect of easy purification and recovery and small molecular weight range

Inactive Publication Date: 2018-11-27
SHANDONG TOPSCI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing physical degradation method has a simple treatment process, but the production efficiency is low, the molecular weight range of the product is not easy to control, and the stability is poor
The chemical degradation method introduces chemical reagents, and the reaction conditions are complicated, which has a great impact on product quality, which is not conducive to the production of small-molecule hyaluronic acid products with stable quality and high purity, and the cost is high in the production process, resulting in a large amount of industrial wastewater. Product recovery rate is low
Bioenzymatic degradation of hyaluronic acid, mild reaction conditions, but high cost, not suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment １

[0023] A method for purifying small molecule hyaluronic acid, comprising the following steps in sequence:

[0024] (1) Slowly pass the hyaluronic acid to be treated through the activated carbon, the time for passing through the activated carbon is 2 hours, and the temperature is 60°C; the average molecular weight of the hyaluronic acid to be treated initially is 800kDa-1100kDa.

[0025] (2) Carry out alcohol precipitation three times in sequence, specifically:

[0026] ① Use ethanol solution with a concentration of 70% for alcohol precipitation, filter out the precipitate and dissolve it with pure water to obtain a solution;

[0027] ②Add ethanol to the solution until the concentration of ethanol is 40%, carry out the second alcohol precipitation, filter to remove the precipitate, add 1% (w / v) KCl to the filtrate, and stir to dissolve;

[0028] ③Add ethanol until the ethanol concentration is 65% for the third alcohol precipitation, collect the hyaluronic acid precipitate, dis...

Embodiment 2

[0034] A method for purifying small molecule hyaluronic acid, comprising the following steps in sequence:

[0035] (1) Slowly pass the hyaluronic acid to be treated through the activated carbon for 1.5 hours at a temperature of 50°C; the average molecular weight of the initially treated hyaluronic acid is 800kDa-1100kDa.

[0036] (2) Carry out alcohol precipitation three times in sequence, specifically:

[0037] ① Use ethanol solution with a concentration of 60% for alcohol precipitation, filter out the precipitate and dissolve it with pure water to obtain a solution;

[0038] ②Add ethanol to the solution until the concentration of ethanol is 30% for the second alcohol precipitation, filter to remove the precipitate, add 0.5-1.5% (w / v) KCl to the filtrate, stir to dissolve;

[0039] ③ Add ethanol until the ethanol concentration is 75% for the third alcohol precipitation, collect the hyaluronic acid precipitate, discard the supernatant, and wash the precipitate with ethanol; ...

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Abstract

The invention discloses a small molecule hyaluronic acid purification method. Small molecule hyaluronic acid is obtained by activated carbon treatment, tertiary alcohol precipitation, activated carbontreatment, centrifugation, rotary evaporation and freeze drying in order. The method is simple in reaction conditions. Furthermore, the method can further improve product purity, reduce impurities inproducts, and is suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of separation and purification of hyaluronic acid, in particular to a method for purifying small molecule hyaluronic acid. Background technique [0002] Small molecule hyaluronic acid has unique characteristics in bio-penetration and biodegradation, and has great application value. At present, the degradation methods of hyaluronic acid mainly include physical degradation, chemical degradation, and biological enzymatic degradation. [0003] The existing physical degradation method has a simple treatment process, but the production efficiency is low, the molecular weight range of the product is not easy to control, and the stability is relatively poor. The chemical degradation method introduces chemical reagents, and the reaction conditions are complicated, which has a great impact on product quality, which is not conducive to the production of small-molecule hyaluronic acid products with stable quality and h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
CPCC08B37/0003C08B37/0072
Inventor 赵贵吉张建勇赵梦喆刘守垒邢仕杰马锡锋王海泉李文源申加秋
Owner SHANDONG TOPSCI BIO TECH
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