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Method for extracting sericin from refined reeling mill silk liquid and ultrafiltration membrane device

A sericin and refining liquid technology, which is applied in the fields of peptide preparation, chemical instruments and methods, animal/human peptides, etc., can solve the problems of equipment damage, easy blockage of ultrafiltration membranes, and reduced use level of sericin, etc. Achieve high recovery rate, improve recycling rate and reduce loss

Pending Publication Date: 2018-12-28
HENAN MINXING COCOON SILK +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method uses 6000-100000 and 1000-6000 ultrafiltration membranes to cooperate with filtration, and finally concentrate and dry. Although this method can obtain sericin protein, the whole process is too simple, and the filtration is relatively large without pretreatment of sericin waste liquid. On the one hand, it will damage the equipment, and the ultrafiltration membrane is easy to block, which increases the production cost. On the other hand, the larger impurities in the sericin concentrate are concentrated and dried because they cannot pass through the ultrafiltration membrane. Contains impurities in sericin, which leads to a reduction in the use level of sericin, which limits the development of sericin products, and is very unfavorable for making sericin products with different functions

Method used

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  • Method for extracting sericin from refined reeling mill silk liquid and ultrafiltration membrane device
  • Method for extracting sericin from refined reeling mill silk liquid and ultrafiltration membrane device
  • Method for extracting sericin from refined reeling mill silk liquid and ultrafiltration membrane device

Examples

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Embodiment 1

[0032] A method for extracting sericin from the refined liquid of mulberry bark, comprising the following steps:

[0033] (1) Sangtiao spit refining liquid is pretreated through 300 mesh, 500 mesh and 800 mesh vibrating sieves successively to remove impurities with larger particle sizes in the refining wastewater to obtain the filtrate;

[0034] (2) The filtrate obtained in step (1) is finely removed with an ultrafiltration membrane with a molecular weight cut-off of 40,000D, and at the same time, sericin with a molecular weight cut-off greater than 40KD is put into the concentrated solution, and finally concentrated to the total volume of the filtrate 10% of the permeate is collected, and the permeate flow rate is 180L / h·m 2 , pressure 0.25MPa, temperature 27°C-32°C;

[0035] (3) Concentrate the permeate in step (2) with a nanofiltration membrane with a molecular weight cut-off of 8000D, and the flow rate of the permeate is 300L / h m 2 , pressure 0.45-0.55MPa, temperature 27...

Embodiment 2

[0054] The present embodiment is basically the same as Example 1, except that: the concentrated solution in step (2) adds papain and deionized water, and the mass ratio of the concentrated solution to papain and deionized water is 100:0.4:800, Enzymolyze at 55-65°C for 40-55min, then heat up to 90-95°C to heat and kill the enzyme, then cool to 27°C-32°C, and carry out steps (2), (3) and (4) in turn for the enzymolysis solution ), to obtain sericin powder.

[0055] Use 12% separation gel for electrophoresis test, see figure 2 As shown, band 1 is the electrophoresis band of sericin protein with a molecular weight of 40KD or more, and bands 2-5 are the electrophoresis bands of sericin protein with a molecular weight of 40KD or more hydrolyzed with papain. After the membrane is refined and slag removed, the molecular weight of sericin in the concentrated solution is above 40KD, and the molecular weight of sericin obtained by hydrolysis with papain is mainly concentrated between ...

Embodiment 3

[0057] This embodiment is basically the same as the second embodiment, except that the mass ratio of the concentrate to papain and deionized water is 100:0.5:800.

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Abstract

The invention provides a method for extracting sericin from a refined reeling mill silk liquid and an ultrafiltration membrane device. The method comprises the following steps: the refined reeling mill silk liquid is preprocessed by three vibrating screens with different pore diameters, and a filtrate is obtained; (2) precise residue removal is performed on the filtrate obtained in step (1) by anultrafiltration membrane, a permeate is collected, and the permeate has the flow of 180 L / h.m<2>, the pressure of 0.25 MPa and the temperature of 27-32 DEG C; (3) the permeate in step (2) is concentrated by a nanofiltration membrane, the permeate has the flow of 300 L / h.m<2>, the pressure of 0.45-0.55 MPa and the temperature of 27-35 DEG C, the permeate is repeatedly washed with deionized water and concentrated for 2-3 h and finally concentrated until the volume is 10% or lower of the total volume of the permeate, and concentrated liquor is collected; (4) the concentrated liquor in step (3) issubjected to spray drying, and the sericin is obtained. The sericin recovery rate is high, the molecular weight of the extracted sericin is determined, and the method is beneficial to sericin development.

Description

technical field [0001] The invention relates to the technical field of production of sericin, in particular to a method and an ultrafiltration membrane device for extracting sericin from mulberry bark refining liquid. Background technique [0002] The silk extracted by the silk reeling factory is called mulberry silk. As the raw material of silk spinning industry, mulberry silk is mainly composed of two parts: silk fibroin and sericin. It is not conducive to the deep processing of silk fabrics, so sericin is usually removed. Sericin covers the surface of silk fibroin, accounting for about 25% of the mass of the cocoon layer, and plays a protective and adhesive role on silk fibroin. Except for a small amount of wax, carbohydrates, pigments and inorganic components, the main component is sericin. Sericin is a globular protein with a relative molecular weight ranging from 1.4-100KD (D is Dalton), and it varies with different degumming methods. Sericin has the properties of a ...

Claims

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Application Information

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IPC IPC(8): C07K14/435C12P21/06C07K1/34
CPCC12P21/06C07K14/43586
Inventor 王志强卢福堂张慧华魏会丽徐斌卢瑶
Owner HENAN MINXING COCOON SILK
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