Application of compound NSC343550 in preparation of drugs for treating or preventing influenza viruses

An influenza virus, a technology for preventing influenza, applied in the direction of active ingredients of heterocyclic compounds, antiviral agents, etc.

Active Publication Date: 2018-12-07
ZHEJIANG GLLION BIOTECH CO LTD
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the emergence of virus resistance to these drugs and the side effects caused by these drugs, the development of new anti-influenza virus drugs is imminent

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of compound NSC343550 in preparation of drugs for treating or preventing influenza viruses
  • Application of compound NSC343550 in preparation of drugs for treating or preventing influenza viruses
  • Application of compound NSC343550 in preparation of drugs for treating or preventing influenza viruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1、293

[0032] Preparation of embodiment 1, 293T-IAV-Luc cells

[0033] 1. Construction of pREP4-IAV-Luc plasmid

[0034] 1. Artificially synthesize the DNA fragment shown in sequence 1 in the sequence listing. Sequence 1 consists of a total of 1748 nucleotides, the 14th-58th position is recorded as fragment 1, the 59th-1711th position is the firefly luciferase coding gene (reporter gene), the 1712-1734th position is recorded as fragment 2, and the two ends It is the recognition site sequence of BsmB I. Wherein, both fragment 1 and fragment 2 are promoters of influenza virus NP protein, and in the presence of influenza virus, the promoter located on the fusion plasmid can be activated, so that firefly luciferase can be expressed.

[0035] 2. Use the restriction endonuclease BsmBI to digest the DNA fragment shown in Sequence 1 in the sequence list, recover the digested fragment and connect it with the large backbone fragment of the pHH21 vector that has undergone the same digestion, an...

Embodiment 2

[0038] Example 2, compound NSC343550 inhibits the replication of H1N1 subtype influenza virus

[0039] 1. Mix A / WSN / 33 strain, DMEM medium and compound NSC343550 to obtain a mixed solution. The mixed solution contained 0.5 MOI virus and 50 μmol / L compound NSC343550.

[0040] 2. Spread the 293T-IAV-Luc cells prepared in Example 1 evenly on a 96-well plate (20,000 cells per well), culture at 37° C. for 12 hours, discard the supernatant, and wash the cells in the well with PBS buffer solution. cell.

[0041] 3. After completing step 2, take the 96-well plate, add the mixed solution obtained in step 1 (MOI=0.1), incubate at 37°C for 1 hour, and discard the supernatant.

[0042] 4. After completing step 3, take the 96-well plate, add DMEM medium containing 10% (volume ratio) fetal bovine serum and 50 μmol / L compound NSC343550, and culture at 37°C for 12 hours, discard the supernatant, and use PBS The buffer washes the cells in the wells.

[0043] 5. After completing step 4, tak...

Embodiment 3

[0048] Embodiment 3, compound NSC343550 suppresses the activity of HA pseudovirus

[0049] 1. Preparation of HA pseudovirus

[0050] 293T cells were inoculated into cell culture dishes, and after reaching 80% density, they were co-transfected with 6 μg pNLLucE-R-HIV-Luc plasmid, 6 μg pEWSN-HA plasmid and 6 μg pCAGGS-NA plasmid with the help of transfection reagent PEI, and replaced with 10 % (volume fraction) DMEM complete medium of fetal bovine serum, after 48 hours, transfer the entire culture system to a 15ml centrifuge tube, blow off the cells, freeze and thaw once, filter with a 0.22 μm filter, and collect the filtrate, which is the HA pseudovirus The virus solution was stored at -80°C.

[0051] 2. Compound NSC343550 inhibits the activity of HA pseudovirus

[0052] 1. Spread 293T cells evenly on a 24-well plate (160,000 cells per well), culture at 37°C for 15 hours, and discard the supernatant.

[0053] 2. After completing step 1, take the 24-well plate, and add 100 μL...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an application of a compound NSC343550 in the preparation of drugs for treating or preventing influenza viruses. The compound NSC343550 is a compound represented by formula (I). The application of the compound NSC343550 is an application in the preparation of the drugs for inhibiting the influenza viruses or an application in the inhibition of influenza viruses. The compound NSC343550 has great values in the prevention and treatment of the influenza viruses.

Description

technical field [0001] The present invention relates to the application of a compound NSC343550 in the preparation of medicines for treating or preventing influenza virus. Background technique [0002] Influenza viruses belong to the genus Influenzavirus of the family Orthomyxoviridae. Influenza viruses are divided into three types: A, B, and C, according to the antigenic and genetic characteristics of the nucleoprotein (NP) and matrix protein (M) of the virion. The whole genome of influenza A virus consists of eight single-stranded negative-strand RNAs of different sizes, named after segment 1 to segment 8, respectively. Influenza A viruses can be further divided into 17 H (H1-H17) and 10 N (N1-N10) subtypes based on the virion surface glycoproteins hemagglutinin (HA) and neuraminidase (NA). . Human influenza viruses are mainly of H1, H2 and H3 subtypes. At present, the highly pathogenic avian influenza with serious harm is mostly H5, H7 and H9 subtypes, among which the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/41A61P31/16
CPCA61K31/41
Inventor 叶昕郭宏亮叶榛庄秀园林檬王轩
Owner ZHEJIANG GLLION BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap