Polypeptide for specifically binding to positive serum of mycoplasma pneumoniae and diagnosis kit

Abstract

The invention provides a polypeptide for specifically binding to positive serum of mycoplasma pneumoniae. The polypeptide has an amino acid sequence T-V-N-F-K-L-Y shown in the formula of SEQ ID NO: 1.The invention provides a mycoplasma pneumoniae infection serological diagnosis kit. The kit contains the polypeptide for specifically binding to positive serum of mycoplasma pneumoniae. The inventionalso provides a mycoplasma pneumoniae diagnosis kit based on a phage display polypeptide. The kit contains a core phage for displaying the polypeptide. The polypeptide and the corresponding core phage have high sensitivity and specificity and a high clinical diagnostic value.

Description

technical field [0001] The invention provides a polypeptide capable of specifically binding to positive serum of Mycoplasma pneumoniae, a diagnostic kit based on the polypeptide, and further relates to a diagnostic kit for Mycoplasma pneumoniae based on a phage display polypeptide, which belongs to the field of biological preparations. Background technique [0002] Mycoplasma pneumoniae (Mycoplasma pneumoniae, MP) is a prokaryotic cell-type organism between viruses and bacteria. The pathological changes caused by its infection are mainly interstitial pneumonia, which accounts for about 1 / 2 of non-bacterial pneumonia in humans and animals. . MP is highly contagious, especially in children, and is the main cause of community-acquired pneumonia. Therefore, early diagnosis of MP pneumonia is extremely important. At present, Mycoplasma pneumoniae infection is mainly diagnosed by the isolation and culture of MP, serological detection, PCR detection and physical diagnosis. There i...

AI Technical Summary

Problems solved by technology

These previous studies were based on traditional protein expression and purification, which was cumbersome, expensive and low specificity

Images