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Corn kernel size gene ZmSWEET1b and application

A technology of gene and corn breeding, applied in the field of corn genetics and breeding

Active Publication Date: 2018-12-07
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, except for the sucrose transporters SUT1 and SUT2 of the SUT family (Slewinski TL, et al. J Exp Bot, 2009, 60(3): 881-892; Leach KA, et al. JIntegr Plant Biol, 2017, 59(6) :390-408), and ZmSWEET4c of the SWEET family, there are no other sugar transporters in maize involved in the source-sink transport of maize photosynthetic products and the formation of grain size

Method used

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  • Corn kernel size gene ZmSWEET1b and application
  • Corn kernel size gene ZmSWEET1b and application
  • Corn kernel size gene ZmSWEET1b and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 The discovery of premature senescence and yield reduction mutants in maize

[0028] Maize Zheng 58 inbred line was mutagenized with EMS (ethyl methylsulfonate) to construct a mutant library with a capacity of 8000 plants, and a mutant early senescence1 (es1 for short) (CGMCC 15471) was obtained in the mutant library. The es1 mutants did not display any visible phenotypes until the grain filling stage. However, from 5 days before silking (5DBS, days before silking), the leaves of es1 began to show a senescence phenotype, which showed that the lower leaves began to chlorosis. By the 15th day after silking (15DAS, days after silking), most of the leaves of es1 showed a chlorotic phenotype ( figure 1 a). Further, the SPAD502 chlorophyll content detector (Konica Minolta) was used to continuously observe the chlorophyll content of the leaves during the filling stage, and it was found that the chlorophyll content of es1 not only decreased earlier than the wild typ...

Embodiment 2

[0030] Example 2 Determination of Maize ES1 Gene

[0031] To clone the ES1 gene, we constructed the es1X B73F 2 Genetic mapping population, the number of wild-type and progeria mutants in the F2 generation mapping population was counted, in 727 F 2 Among the individuals, 169 individuals exhibited es1 phenotype, and 558 individuals exhibited wild-type phenotype. The chi-square test results indicated that the genetic mapping population of the F2 generation did not deviate from the 3:1 Mendelian segregation ratio (χ 2 =1.192 0.05 = 3.84) (Table 1). Therefore, it is a single recessive mutation that controls this progeria trait.

[0032] Table 1 Chi-square test results

[0033]

[0034] Map-based cloning of the es1 gene was carried out. The first step was to use BSA (Bulked Segregant Analysis) to search for molecular markers linked to es1 in order to determine the approximate position of es1 in the maize genome. According to different phenotypes, the genomic DNA of prematur...

Embodiment 3

[0041] Example 3 Maize ZmSWEET1b gene analysis

[0042] The GRMZM2G153358 gene information was retrieved from the maize MaizeGDB website B73 RefGen_V3 (https: / / www.maizegdb.org). GRMZM2G153358 is the gene of corn sugar transporter ZmSWEET1b, located on the long arm of chromosome 6 in maize. Its genome sequence (Genomic DNA) is shown in SEQ ID NO.1, its cDNA sequence is shown in SEQ ID NO.2, and the translated protein sequence is shown in SEQ ID NO.3. The ZmSWEET1b gene has 6 exons and 5 introns, encoding 250 amino acids. The protein structure analysis software PROTTER (http: / / wlab.ethz.ch / protter / start / ) was used to predict the structure of the ZmSWEET1b protein. The analysis results showed that the ZmSWEET1b protein has 7 transmembrane regions, and the N-terminal is located outside the cell membrane. The end is located inside the cell membrane, and in the es1 mutant is the glutamine E of the third transmembrane region of the ZmSWEET1b gene 81 Mutation to lysine K 81 ( Fi...

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Abstract

The invention relates to a corn kernel size gene ZmSWEET1b and an application and belongs to corn genetic breeding. The amino acid sequence of a sugar transporter ZmSWEET1b is as shown in SEQ ID NO: 3. Experimental results verify that the corn ZmSWEET1b gene is a gene which affects the corn kernel size. The ZmSWEET1b is utilized reasonably to improve the corn output, and high and stable yield breeding is carried out.

Description

technical field [0001] The invention belongs to the field of corn genetics and breeding, and specifically relates to a gene ZmSWEET1b affecting corn leaf senescence and grain size and its application Background technique [0002] During the growth and development of plants, if an organ produces more photosynthetic products than it consumes, such an organ is called a "source"; conversely, if an organ consumes or stores more photosynthetic products than it produces Such organs are called "sinks"; the transportation of photosynthetic products from "source" to "sink" is called "flow" (Herbers K and Sonnewald U.Curr Opin Plant Biol,1998,1(3):207 -216; Borchers-Zampini C, et al. Plant Physiol, 1980, 65(6):1116-1120). The source and sink of maize during the grain-filling stage are leaves and ears, respectively, and during this period, the growth of the ears ("sinks") depends on the leaves ("sources") continuously supplying it with photosynthetic products. Therefore, the "source-s...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8261
Inventor 郎志宏汪海李圣彦滕守振张昊辛红佳
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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