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Device and method for loading freeze-drying protection agent on red blood cells

A technology for freeze-drying protective agent and red blood cells, which is applied in the field of medical devices to achieve the effect of improving survival rate, recovery rate and good convenience.

Pending Publication Date: 2018-12-07
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, the above methods usually require multi-step operations and long processing

Method used

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  • Device and method for loading freeze-drying protection agent on red blood cells
  • Device and method for loading freeze-drying protection agent on red blood cells
  • Device and method for loading freeze-drying protection agent on red blood cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] The first, second and third chips of the device loaded with lyoprotectant (trehalose) on red blood cells in this embodiment are square plexiglass chips with a length of 12 cm and a width of 7 cm.

[0080] The first channel provided on the lower surface of the first chip is distributed in an S-shaped manner, including a longitudinal channel and a transverse channel perpendicular to the longitudinal channel, and the transverse channel and the longitudinal channel are connected. Through hole 1 is the inlet of cell suspension, and through hole 2 is the inlet of hypotonic solution. An S-shaped mixer with a length of about 12.7 cm is arranged at the entrance of the first channel, so that the hypotonic solution and the red blood cell suspension are mixed here, so that the red blood cells form holes in the red blood cell membrane in the hypotonic solution, and the trehalose solution passes through the hole and enters In the red blood cells, it flows through the hypertonic point...

Embodiment 2

[0086] The device for loading lyoprotectant (trehalose) on red blood cells provided in Example 1 is used to load trehalose on red blood cells, and the specific process is as follows:

[0087] Collect erythrocytes, mix erythrocytes and normal saline evenly to obtain a cell suspension with a volume ratio of 35±5%;

[0088]The cell suspension is poured into the inlet 1 of the first channel of the device loaded with lyoprotectant (trehalose) provided in Example 1, and the hypotonic trehalose solution is poured from the first channel Inlet 2 of the inlet, the cell suspension and the hypotonic trehalose solution flow along the first channel, mix evenly at the S-type mixer, and the mixed solution flows into the second channel; the hypertonic trehalose solution flows from the second The entrance 3 of the channel is poured, and the mixed solution and hypertonic trehalose solution are mixed evenly at the S-shaped mixer; the mixed solution enters the material exchange area of ​​the third...

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Abstract

The invention provides a device and method for loading a freeze-drying protection agent on red blood cells. The device comprises a first chip, a second chip, a third chip, at least four first porous films and at least one second porous film, wherein a first channel is arranged on the lower surface of the first chip; the first channel comprises a plurality of inlets and a first mixer; the second chip is located below the first chip and a second channel is arranged on the upper surface of the second chip; the second channel comprises an inlet and a second mixer, and a third channel is arranged on the lower surface of the second channel; the third channel comprises a substance exchange region; the third chip is located below the second chip and a fourth channel is arranged on the upper surface of the third chip; the fourth channel comprises a substance exchange region; the first channel of the first chip is communicated with the second channel of the second chip; and the second channel ofthe second chip is communicated with the third channel. According to the device and method for loading the freeze-drying protection agent on the red blood cells, the freeze-drying protection agent can be effectively and automatically loaded into the red blood cells, and the freeze-drying survival rate and recycling rate of the red blood cells are improved; and the device and the method have relatively good convenience.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a device and method for carrying a freeze-drying protective agent on red blood cells. Background technique [0002] Freeze-drying is a common method for long-term preservation of red blood cells (RBCs), especially for the preservation of precious cells. It has many advantages over traditional methods, such as room temperature storage, stable performance, and easy transportation. In order to reduce the low-temperature damage of cells during the freeze-drying process, it is often necessary to add a certain concentration of a certain freeze-drying protective agent to the preserved cell suspension. Maintaining the integrity of the erythrocyte membrane is an important prerequisite for the lyophilization of erythrocytes. Trehalose is a naturally occurring disaccharide. Studies have shown that the presence of trehalose on both sides of the cell membrane can enhance the toleranc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/00
CPCC12M23/16C12M25/00
Inventor 丁卫平杜坤沈依韧邹丽丽刘婧
Owner UNIV OF SCI & TECH OF CHINA
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