Stem cell culture medium and culture method

Abstract

The invention relates to a stem cell culture medium which comprises a basic culture medium and an additive. The additive contains 1-200 [mu]g / mL of recombinant transferring, 1-300 [mu]g / mL of recombinant albumin, 0.1-200 ng / mL of recombinant cell factors, 10-300 [mu]g / mL of free fatty acids, 0.1-10 mg / mL of a protease inhibitor, 20-300 [mu]g / mL of vitamins, 0.5-10 mg / Ml of zinc sulfate, 0.01-1 mmol / L of tretinoin, 10-60 nmol / L of sodium selenite and 5-100 [mu]g / mL of niacinamide. The invention also discloses a stem cell culture method which carries out three-dimensional culture on stem cells with a serum-free stem cell culture medium. According to the culture medium and the culture method provided by the invention, the applicability of the culture medium is expanded, and meanwhile, a stable growing environment is provided for stem cells, so that the stem cell culture efficiency is improved obviously and the using cost of the culture medium is lowered.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a stem cell culture medium and a culture method. Background technique [0002] Stem cells refer to a type of cells from embryos, fetuses, and adults that have the ability to self-renew, reproduce, and differentiate into various cells. They are the initial source of cells in the body and other tissues. These cells can differentiate into various Tissue cells are poor and lacking cell sources in the field of biomedicine, and are of great significance for clinical applications. Therefore, the research on stem cells has become a hot spot in biomedical research in recent years. Since the content of stem cells in body tissues is very small, culturing and expanding stem cells in vitro to make the number of stem cells meet the needs of stem cell-related functions and mechanisms is a key technology in the field of stem cell research. [0003] At present, the medium used for stem cell culture ...

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Problems solved by technology

[0007] Chinese patent CN108220231A "a stem cell culture medium and its preparation method and application" discloses a stem cell culture medium and its preparation method and application. Amides, non-essential amino acids and liver cytokines, this invention effectively solves the technical defect that the stem cell medium in the prior art is not suitable for culturing menstrual blood stem cells

[0008] At present, the serum-free stem cell medium is in the process of continuous development, and there are many shortcomings. For example, the current medium can only be used for the cultivation of one or a few kinds of stem cells, which lacks versatility; in addition, there is a cost of use Disadvantages such as relatively expensive

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