Stem cell culture medium and culture method

A technology of stem cells and culture medium, applied in the direction of cell culture active agent, culture process, tissue culture, etc., can solve the problems of lack of versatility, unsuitability for culturing menstrual blood stem cells, and high cost of use, so as to reduce the complexity of ingredients, clear ingredients, Quality Controlled Effects

Active Publication Date: 2018-12-07
广东卡丝股权投资集团有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0007] Chinese patent CN108220231A "a stem cell culture medium and its preparation method and application" discloses a stem cell culture medium and its preparation method and application. Amides, non-essential amino acids and liver cytokines, this invention effectively solves the technical defect that the stem cell medium in the prior art is not suitable for cultur

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  • Stem cell culture medium and culture method
  • Stem cell culture medium and culture method
  • Stem cell culture medium and culture method

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Effect test

Embodiment 1

[0031] Embodiment 1 Serum-free stem cell accompanying medium

[0032] Serum-free stem cell medium, including basal medium and supplements.

[0033] Wherein, the basal medium is a low-sugar DMEM medium, and the types and amounts of additives are:

[0034]

[0035]

Embodiment 2

[0036] Embodiment 2 Serum-free stem cell accompanying medium

[0037] Serum-free stem cell medium, including basal medium and supplements.

[0038] Among them, the basic medium is DMEM / F12 (1:1) medium, and the types and amounts of additives are:

[0039]

Embodiment 3

[0040] Embodiment 3 Serum-free stem cell accompanying medium

[0041] Serum-free stem cell medium, including basal medium and supplements.

[0042] Wherein, the basal medium is a low-sugar DMEM medium, and the types and amounts of additives are:

[0043]

[0044]

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Abstract

The invention relates to a stem cell culture medium which comprises a basic culture medium and an additive. The additive contains 1-200 [mu]g/mL of recombinant transferring, 1-300 [mu]g/mL of recombinant albumin, 0.1-200 ng/mL of recombinant cell factors, 10-300 [mu]g/mL of free fatty acids, 0.1-10 mg/mL of a protease inhibitor, 20-300 [mu]g/mL of vitamins, 0.5-10 mg/Ml of zinc sulfate, 0.01-1 mmol/L of tretinoin, 10-60 nmol/L of sodium selenite and 5-100 [mu]g/mL of niacinamide. The invention also discloses a stem cell culture method which carries out three-dimensional culture on stem cells with a serum-free stem cell culture medium. According to the culture medium and the culture method provided by the invention, the applicability of the culture medium is expanded, and meanwhile, a stable growing environment is provided for stem cells, so that the stem cell culture efficiency is improved obviously and the using cost of the culture medium is lowered.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a stem cell culture medium and a culture method. Background technique [0002] Stem cells refer to a type of cells from embryos, fetuses, and adults that have the ability to self-renew, reproduce, and differentiate into various cells. They are the initial source of cells in the body and other tissues. These cells can differentiate into various Tissue cells are poor and lacking cell sources in the field of biomedicine, and are of great significance for clinical applications. Therefore, the research on stem cells has become a hot spot in biomedical research in recent years. Since the content of stem cells in body tissues is very small, culturing and expanding stem cells in vitro to make the number of stem cells meet the needs of stem cell-related functions and mechanisms is a key technology in the field of stem cell research. [0003] At present, the medium used for stem cell culture ...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0663C12N2500/12C12N2500/22C12N2500/24C12N2500/32C12N2500/36C12N2500/38C12N2500/90C12N2501/11C12N2501/115C12N2501/12C12N2501/13C12N2501/135C12N2501/15C12N2501/734C12N2501/998C12N2501/999
Inventor 谢志辉廖云麒许亮
Owner 广东卡丝股权投资集团有限公司
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