A kind of morphological determination and analysis method of Acer genus seeds
An analysis method and seed technology, applied in measuring devices, instruments, optical devices, etc., can solve the problems of inability to fully reflect the shape of seeds, inability to perform accurate measurements, and large human subjective factors, and achieve high accuracy of detection results. , excluding human subjective factors, the effect of strong repeatability
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[0110] According to a preferred embodiment of the present invention, the dominant factor is a factor whose explanatory degree to all morphological variations of Acer seeds is higher than 70%.
[0111] In the present invention, the leading factor obtained is the most important factor affecting the morphological variation of Acer seeds.
[0112] Wherein, the dominant factors include at least two.
[0113] In step 5.3, according to the dominant factors obtained in step 5.2, the measured geometric morphological data of several Acer seeds are divided to evaluate their morphological diversity and determine important germplasm resource areas.
[0114] In the present invention, according to the dominant factor, the measured geometric shape data of Acer seeds are re-differentiated, and the seeds with large shape variation and small variation can be distinguished according to the main influencing factors.
[0115] Among them, taking the two dominant factors (PC1 and PC2) with the highe...
Embodiment 1
[0157] The seeds measured in this embodiment are 5 Acer catalpa seeds randomly selected.
[0158] (1) Press the seeds of Acer catalpa into specimens, place a scale on one side, and then scan with a CanoScan 5600F scanner with a resolution of 300dpi and a scanned page area of 2.867×10 -4 cm 2 .
[0159] (2) Input the scanned image, adjust the minimum threshold value to 0, and the maximum threshold value to 100, and perform binarization on the image, so that the gray level difference between the image of Acer seeds and the background is obvious, and the overall pixel blocks of the seeds and the scanned pages are counted. Pixel blocks, and obtain the measured value of the seed area according to the area of the scanned page (repeated measurement 3 times);
[0160] Select the pixel block at the center of the binding point outside the left and right sides of the Acer catalpa seed and the pixel block at the center of the most prominent point on the outside of the fruit wing on ...
Embodiment 2
[0164] The seeds of the genus Acer used in this embodiment are the seeds of Acer catalpa, and the seeds are from 6 distribution areas (Dujiangyan (dj), Mount Emei (em), Pingwu (pw), Dayi (dy), Longquanyi (LQ), 20 plants in Qionglai (QL)) with normal growth and no obvious defects, diseases and insect pests, and 10 seeds were collected from each tree.
[0165] (1) Press the seeds of Acer catalpa into specimens, and then scan them with a CanoScan 5600F scanner with a resolution of 300dpi;
[0166] Select an image, adjust the image of Acer catalpa seeds to the minimum threshold value of 0, and the maximum threshold value of 100, so that the pixel blocks of the edge, tip and inflection point of the seed in the image are clearly identifiable, and the reference points LM1~ LM16, such as Figure 5 As shown in , the location information of the reference point is as follows:
[0167] The lower junction point (LM1) at the junction of the left and right sides of the samara, the upper ju...
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