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A kind of method adopting sodium alginate to carry out pathological film preparation

A sodium alginate and pathological technology, applied in the field of pathological diagnosis, can solve the problems of difficult diagnosis, small amount of tissue cells in specimens, and difficulty in obtaining tissue specimens, and achieve the effect of improving the accuracy of diagnosis

Active Publication Date: 2021-08-10
上海交通大学附属第一人民医院松江分院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In clinical practice, it is often the case that it is extremely difficult to obtain tissue samples due to the complexity of the disease, such as puncture under the guidance of various imaging studies, urine from catheterization, a small amount of pleural effusion, drainage fluid, etc., sometimes because the patient The site of the disease is special, far away from the body surface or hidden under other tissues and organs, and the amount of specimen obtained is small or the amount of tissue cells contained in the specimen is small. Generally, only cytological smear detection and cytological diagnosis can be performed, and there is no possibility of diagnosis. Repeatability and the possibility of further testing of new technologies
Cytological examination is simple and widely used. In most cases, only qualitative cytological diagnosis can be made, but sometimes it is difficult to diagnose

Method used

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  • A kind of method adopting sodium alginate to carry out pathological film preparation
  • A kind of method adopting sodium alginate to carry out pathological film preparation
  • A kind of method adopting sodium alginate to carry out pathological film preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The concentration of each substance added was divided into the following groups, and the specific results are shown in Table 1.

[0018] Table 1

[0019]

[0020] Take 2ml of calcium chloride solution, quickly add 1ml of sodium alginate solution, shake well and let it stand for 30 seconds to form a ball, then pour it out, pump out the liquid in the ball, inject 0.5ml of tissue cell suspension, wrap it with gauze and put it in The embedding cassettes were dehydrated and sliced.

[0021] Wherein, the preparation method of isopropanol sodium alginate is as follows: first prepare 0.5%, 1%, 3% sodium alginate solution, and then add 0.1 ml of isopropanol to 10 ml of the above-mentioned concentration of sodium alginate solution.

[0022] Set the groups shown in Table 2 according to the concentrations in Table 1, and measure the ball forming, residue, dyeing effect and fixation time.

[0023] Table 2

[0024]

[0025]

[0026] In the table above:

[0027]

[002...

Embodiment 2

[0030] The concentration of each substance added was divided into the following groups, and the specific results are shown in Table 3.

[0031] table 3

[0032]

[0033] Add 1ml of sodium alginate solution into the round hole of the mold, inject 0.5ml of eosin-stained tissue cell suspension, immediately put it into 2ml of calcium chloride ethanol glacial acetic acid solution to form a gel, wrap it with gauze and put it into the embedding box Carry out dehydration and tablet making.

[0034] Set the groups shown in Table 4 according to the concentration in Table 3, and measure the ball forming, residue, dyeing effect and fixation time.

[0035] Table 4

[0036] Packaged residual Dyeing effect post-fixation time A1D3 +++ + +++ 30 minutes A2D3 +++ + ++ 30 minutes

[0037] The results showed that after wrapping the cells with sodium alginate and fixing them for a short period of time, good results could be achieved. There will be a s...

Embodiment 3

[0039] The added concentration of each substance was divided into the following groups, and the specific results are shown in Table 5.

[0040] table 5

[0041]

[0042] Take a liquid sample containing cell tissue, add eosin for staining, centrifuge at 2000 rpm for 3 minutes, discard the supernatant, keep 0.5ml of the precipitate, directly add 0.5ml of sodium alginate solution to cover the precipitate, and then add 2ml of calcium chloride solution Shape the gel, wrap it with gauze and put it into the embedding box for dehydration and tablet production.

[0043] Set the groups shown in Table 6 according to the concentration in Table 5, and measure the ball forming, residue, dyeing effect and fixation time.

[0044] Table 6

[0045] Packaged residual Dyeing effect post-fixation time A1C3 +++ + / - +++ 30 minutes A2C3 +++ + / - ++ 30 minutes

[0046] where residual + / - extremely low

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Abstract

The present invention relates to the technical field of pathological diagnosis, in particular to a method for making pathological slices using sodium alginate, which forms a gel with sodium alginate solution and calcium chloride solution to coat tissue cell suspension; the present invention can A small amount of tissue cells are aggregated to convert cytological diagnosis into pathological histological diagnosis, so as to overcome the shortcomings of the existing technology. Greatly improve the diagnostic accuracy.

Description

technical field [0001] The invention relates to the technical field of pathological diagnosis, in particular to a method for making pathological slices by using sodium alginate. Background technique [0002] Cytopathological examination is currently a means of examination with diagnostic significance. Clinical cytology examination includes body fluid exfoliation cell examination, such as sputum, urine sediment, pleural effusion, ascites cytology examination and vaginal smear examination, etc.; Cervical smears and tumor surface decellularization under endoscopy; fine-needle aspiration cytology, such as using a needle and syringe to aspirate tumor cells for smear staining examination, etc. In clinical practice, it is often the case that it is extremely difficult to obtain tissue samples due to the complexity of the disease, such as puncture under the guidance of various imaging studies, urine from catheterization, a small amount of pleural effusion, drainage fluid, etc., some...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/30G01N1/36
CPCG01N1/2813G01N1/30G01N1/36
Inventor 邱承敏钱秀红
Owner 上海交通大学附属第一人民医院松江分院
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