Test strip for detecting ketamine and preparation method and application method thereof

A test strip and ketamine technology, applied in the field of ketamine detection, can solve the problems of inability to realize rapid detection of large-scale samples, time-consuming, complicated operation, etc., and achieve the effects of short detection time, simple storage, and simple operation.

Inactive Publication Date: 2018-12-07
郑州左安检测科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the existing methods for detecting ketamine, which are complex in operation, time-consuming, and unable to realize rapid detection of large batches of samples, and provide a method with s

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  • Test strip for detecting ketamine and preparation method and application method thereof
  • Test strip for detecting ketamine and preparation method and application method thereof
  • Test strip for detecting ketamine and preparation method and application method thereof

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preparation example Construction

[0041] The preparation method of the test strip for detecting ketamine mainly comprises the following steps:

[0042] 1) Label the ketamine antibody with fluorescent microspheres, and spray it on the conjugate release pad to prepare the conjugate release pad sprayed with fluorescent microsphere-labeled ketamine antibody;

[0043] 2) Spray the hapten-carrier protein conjugate and goat anti-mouse secondary antibody on the reaction membrane respectively as the test line (T) and quality control line (C) to prepare the ketamine-coated hapten-carrier protein conjugate The detection line of the substance and the reaction membrane of the quality control line coated with goat anti-mouse secondary antibody;

[0044] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the bottom plate into a test strip.

[0045] The following step-by-step detailed description:

[0046] 1. Preparation of fluorescent ...

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Abstract

The invention discloses a test strip for detecting ketamine and a preparation method and application method thereof. The test strip orderly comprises a sample absorption pad, a conjugate release pad,a reaction film, a water absorbent pad and a bottom plate. The conjugate release pad is spray-coated with a time-resolved fluorescent microsphere-labeled antibody. The reaction film is coated with a hapten-carrier protein conjugate detection line and a goat anti-mouse secondary antibody quality control line. Through the time-resolved fluorescent microsphere-labeled antibody and immunochromatography, ketamine can be used for fast immunoassay. The invention also provides a method for detecting ketamine in a sample through the test strip. The test strip has the advantages of high sensitivity, accurate quantification, rapid detection, convenient operation, economical and practical effects, and can realize rapid detection and on-site detection of a large amount of ketamine samples.

Description

technical field [0001] The invention relates to the detection of ketamine, in particular to a test strip for quantitatively detecting ketamine by using time-resolved fluorescent microsphere immunochromatography technology and a preparation method and an application method thereof. Background technique [0002] Ketamine, a dissociative anesthetic, is a derivative of phencyclidine (PCP), commonly known as "K powder", and is generally used as an anesthetic in clinical practice. Ketamine can excite blood vessels, overdose can be fatal, and has certain mental dependence. After "K powder" becomes addicted, under the influence of the drug, the addict will shake his head frantically, which can easily break the spine; at the same time, the crazy shaking can also cause heart and respiratory failure. Excessive or long-term smoking can cause fatal damage to the heart, lungs, and nerves, and the damage to the central nervous system is more severe than methamphetamine. [0003] The curr...

Claims

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Application Information

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IPC IPC(8): G01N33/533G01N33/558G01N33/58G01N33/94
CPCG01N33/533G01N33/558G01N33/582G01N33/946
Inventor 吕小翠张振兴贾嘉赵琳周亮崔浩哲张鹏高翔
Owner 郑州左安检测科技有限公司
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