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Simplified production method for rape male sterile hybrid F1 seeds

A technology of male sterility and production methods, applied in the fields of botany equipment and methods, plant genetic improvement, application, etc., can solve the problems of high labor cost, time-consuming and labor-intensive, etc., and achieve the effect of reducing risks

Active Publication Date: 2018-12-18
武汉联农种业科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, the existing methods are time-consuming and labor-intensive, and the labor cost is relatively high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Breeding of Polyma cytoplasmic male sterile restorer line 7-5SR with self-incompatibility

[0018] (1) Use the restorer line 7-5 of Huayouza 12 as the female parent (genotype bnS-1300bnS-1300) and a self-incompatible individual plant isolated from the F2 offspring of Huayouza 95 (genotype F1 was obtained by hybridizing to BnS-1300 (BnS-1300).

[0019] (2) Use the above F1 as the female parent to backcross with 7-5 to obtain BC1.

[0020] (3) Use the DNA molecular markers linked to the self-incompatibility gene BnS-1300 to perform DNA molecular detection on the above-mentioned BC1 population individual plants, and screen out the heterozygous genotype (BnS-1300bnS) at the self-incompatibility gene locus -1300) per plant.

[0021] (4) Use the above-mentioned heterozygous single plant as the male parent to backcross with 7-5 to obtain BC2.

[0022] (5) Use the DNA molecular marker linked to the self-incompatibility gene BnS-1300 to perform DNA molecular detect...

Embodiment 2

[0029] Example 2: Rapeseed Cytoplasmic Male Sterile Hybrid Seed Production

[0030] The method of the present invention comprises utilizing the cytoplasmic male sterile line 616A and the restorer line 7-5SR having self-incompatibility to produce hybrid F1 seeds, and the specific steps of the hybrid seed production method include:

[0031] (1) The seeds of the cytoplasmic male sterile line 616A and the restorer line 7-5SR with self-incompatibility were mixed evenly at a ratio of 80%:20%.

[0032] (2) Under the isolation condition of the artificial net room, the above-mentioned uniformly mixed seeds are planted with a row spacing of 20 cm and a plant spacing of 10 cm.

[0033] (3) The sterile line and the restorer line were mixed and harvested when the seeds were mature, and statistics showed that the hybrid purity was 92.6%.

Embodiment 3

[0034] Example 3: Rapeseed Cytoplasmic Male Sterile Hybrid Seed Production

[0035] (1) The seeds of the nuclear male sterile line 616A and the restorer line 7-5SR with self-incompatibility were mixed evenly at a ratio of 80%:20%.

[0036] (2) Under natural isolation conditions, the above-mentioned uniformly mixed seeds were planted at a density of 25,000 plants / mu.

[0037] (3) The sterile line and the restorer line were mixed and harvested when the seeds were mature, and statistics showed that the hybrid purity was 90.6%.

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Abstract

The invention provides a simplified production method for rape male sterile hybrid F1 seeds. The method is a hybridization seed production method for producing the hybrid F1 seeds by selecting a cytoplasmic male sterile line (or a nuclear male sterile line) and a restorer line having a self-incompatibility characteristic. The method concretely comprises the following steps: mixing the cytoplasmicmale sterile line (or the nuclear male sterile line) and restorer line seeds having the self-incompatibility characteristic according to a specific ratio under an isolation condition, sowing the obtained uniformly-mixed seeds in the field of a rape seed production area, and harvesting the sterile line and the restorer line in a mixed manner at the time of ripening to obtain the hybrid F1 seeds. The method has the advantages of simple breeding program, saving of the labor cost, and saving of money and labor.

Description

technical field [0001] The invention belongs to the technical field of rapeseed hybrid F1 seed production, in particular, relates to a hybrid seed production using a cytoplasmic male sterile line or a nuclear male sterile line and a restorer line with self-incompatibility characteristics to produce hybrid F1 seeds method. Background technique [0002] Rapeseed has significant heterosis, which can generally increase yield by 20%-30%, and a good combination can increase yield by 50%. The popularization and application of hybrid rapeseed is developing rapidly. Only the area of ​​hybrid rapeseed in my country has accounted for more than 70% of the total area of ​​rapeseed. Utilization of heterosis is an important way to increase crop yield. There are many ways to utilize heterosis in rapeseed, mainly including cytoplasmic male sterility, nuclear male sterility, chemical male sterility and so on. [0003] Rapeseed male sterility includes cytoplasmic male sterility and nuclear m...

Claims

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Application Information

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IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 杨光圣董发明辛强洪登峰
Owner 武汉联农种业科技有限责任公司
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