A kind of ph-sensitive agricultural fungicide
A technology for using bacterial agents and agriculture, which is applied in the field of pH-sensitive agricultural bacterial agents, which can solve problems such as unfavorable crop growth, reduced fertilizer use efficiency, and fertilizer loss, so as to improve nutrient supply, improve soil salinization, The effect of inhibiting activity
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Embodiment 1
[0045] Embodiment 1, the preparation of pH-sensitive agricultural bacterial agent
[0046] (1) Preparation of agricultural fungicide granules
[0047] Add Bacillus subtilis, yeast, lactic acid bacteria, starch, and water with a mass ratio of 2:2:2:2:1 to a high-speed stirring granulator to granulate, boil and dry at 30-37°C to a water content of 3-5%, and pass 20 Mesh sieve to obtain agricultural fungus granules.
[0048] (2) Dopamine is coated on the surface of agricultural fungus granules
[0049] Weigh 1.5g Tris and place it in a beaker, add about 900mL of deionized water, adjust the pH of the Tris-HCl solution to 8-8.5 with 6M HCl; add 50g of the above-prepared agricultural fungus granules to the above-mentioned Tris-HCl solution, and dilute the solution to 1 L; add 5 g of dopamine hydrochloride, stir at room temperature for more than 2 hours, and suction filter to obtain agricultural fungicide particles coated with dopamine on the surface.
[0050] (3) pH-sensitive pol...
Embodiment 2
[0053] Embodiment 2, the living bacteria release experiment of pH-sensitive agricultural bacterial agent
[0054] Get 6g of pH-sensitive agricultural fungal agent granules prepared according to the method described in Example 1, divide them into 3 parts on average, place them in sterile water with a pH value of 5, 7, and 9 for 1 day, and then detect different pH values. In some cases, the release amount of live bacteria of the pH-sensitive bacterial agent.
[0055] Plate detection method to detect the release of viable bacteria detection method:
[0056] 1) Prepare a solution according to the mass volume ratio of the placed sample: sterile water at a ratio of 1:10, and filter the solution through a 3-micron filter membrane to filter out the dopamine attached to the surface of the unreleased live bacteria Bacteria particles and bacteria particles coated with polymer molecules;
[0057] 2) Collect the filtrate, add glass beads, let it stand for 20 minutes, and shake it on a ro...
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