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Method for extracting hericium erinaceus polysaccharide by internal boiling
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A technology of Hericium erinaceus polysaccharide and Hericium erinaceus powder is applied in the field of polysaccharide extraction to achieve the effects of high extraction effect, good application prospect and high extraction rate
Active Publication Date: 2018-12-25
福州海锐黎思生物科技有限责任公司
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Embodiment 1
[0045] 1) Dry and crush the fruiting bodies of Hericium erinaceus, pass through a 100-200 mesh sieve, and then add an ethanol solution with a mass concentration of 50wt% (containing 3wt% ether ), ultrasonic treatment at 5~10°C for 8 minutes to fully infiltrate Hericium erinaceus powder;
[0046] 2) Add hot water at 80°C in the amount of 20mL / g Hericium erinaceus powder to the liquid obtained in step 1), extract for 4 minutes, and then filter;
[0047] 3) Add 1 / 4 of the volume of absolute ethanol to the filtrate obtained in step 2), remove the supernatant after standing for 8 hours, and dry the obtained precipitate to obtain Hericium erinaceus polysaccharide, and the polysaccharide yield is 12.9%.
Embodiment 2
[0049] 1) Dry and crush the fruiting bodies of Hericium erinaceus, pass through a 100-200 mesh sieve, and then add an ethanol solution with a mass concentration of 70wt% (containing 2wt% ether ), ultrasonic treatment at 5~10°C for 5 minutes to fully infiltrate Hericium erinaceus powder;
[0050] 2) Add hot water at 90°C in the amount of 20mL / g Hericium erinaceus powder to the liquid obtained in step 1), extract for 4 minutes, and then filter;
[0051] 3) Add 1 / 2 of the volume of absolute ethanol to the filtrate obtained in step 2), remove the supernatant after standing for 6 hours, and dry the obtained precipitate to obtain Hericium erinaceus polysaccharide, and the polysaccharide yield is 13.7%.
Embodiment 3
[0053] 1) After drying and crushing the fruiting body of Hericium erinaceus, pass through a 100-200 mesh sieve, and then add an ethanol solution with a mass concentration of 60wt% (containing 1wt% ether ), ultrasonic treatment at 5~10°C for 6 minutes to fully infiltrate Hericium erinaceus powder;
[0054] 2) Add hot water at 80°C in the amount of 20mL / g Hericium erinaceus powder to the liquid obtained in step 1), extract for 5 minutes, and then filter;
[0055] 3) Add 1 / 2 of the volume of absolute ethanol to the filtrate obtained in step 2), remove the supernatant after standing for 7 hours, and dry the obtained precipitate to obtain Hericium erinaceus polysaccharide, and the polysaccharide yield is 15.4%.
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Abstract
The invention discloses a method for extracting hericium erinaceus polysaccharide by internal boiling. The method comprises the steps of after drying and pulverizing hericium erinaceus sporocarp, wetting the hericium erinaceus sporocarp by using 50-70wt% of an ethanol solution at first, then adding hot water at the temperature of 80-100 DEG C and extracting, and filtering the obtained extracting solution and carrying out alcoholprecipitation to obtain the hericium erinaceuspolysaccharide. Compared with an existing method for extracting hericium erinaceuspolysaccharide, the method for extracting hericium erinaceus polysaccharide by internal boiling has the advantages of high extracting speed and simplicity and convenience in operation, is good in extracting rate, and has good applicationprospect.
Description
technical field [0001] The invention belongs to the technical field of polysaccharide extraction, and in particular relates to a method for extracting Hericium erinaceus polysaccharide by internal boiling. Background technique [0002] The internal boiling method is to use a small amount of organic solvent with low boiling point to wet the extracted material to fully desorb the active ingredients, and then add a hot solvent whose temperature is higher than the boiling point of the desorbent, so that the desorbent inside the extracted material is quickly heated to boiling to generate convection , to promote the diffusion of active ingredients and strengthen the extraction process. [0003] Hericium erinaceus polysaccharide is an effective active ingredient extracted from the fruiting body of Hericium erinaceus. Modern medical research shows that Hericium erinaceus polysaccharides have physiological functions such as improving immunity, anti-tumor, anti-aging, and lowering bl...
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