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Quick cultivation method for regenerated plantlets of cremastra appendiculata

A technique for regenerating plants and cultivation methods, which is applied in the field of rapid cultivation of rhododendron orchid regenerated plants, can solve problems such as the long cultivation period of rhododendron orchid seedlings, and achieve the effect of solving the shortage of plant resources and seedling resources and shortening the time

Active Publication Date: 2019-01-01
CHENGDU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Above-mentioned three kinds of methods all have the problem that the rhododendron orchid seedling cultivation period is longer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The rapid cultivation method of the rhododendron orchid regeneration plant that the present embodiment provides comprises the steps:

[0023] (1) Cultivation of rhododendron orchid bulbs

[0024] a. Select seeds from ripe and freshly cracked rhododendron orchid fruits, soak the seeds in 4.0% calcium hypochlorite solution for 30 minutes, then put them into 7.0% hydrogen peroxide solution for disinfection for 5 minutes, Then, after washing with sterile water for 4 times, insert into White + activated carbon 0.8g / L + adenine 7mg / L + sucrose 28g / L liquid medium, and induce culture for 30 days at a temperature of 20°C and without light;

[0025] b. Transfer the seeds after induction culture for 30 days into 1 / 2MS+6BA2.0mg / L+TDZ0.4mg / L+NAA0.3mg / L+activated carbon 1.5g / L medium, at a temperature of 24°C and light every day 20 hours, under the condition of light intensity 2500lx, cultivated for 50 days, the base of the rhododendron orchid bud body of 87% had corms growing;

...

Embodiment 2

[0035] The rapid cultivation method of the rhododendron orchid regeneration plant that the present embodiment provides comprises the steps:

[0036] (1) Cultivation of rhododendron orchid bulbs

[0037] a. Select seeds from ripe and freshly cracked rhododendron orchid fruits, soak the seeds in 2.0% calcium hypochlorite solution for 20 minutes, then put them into 5.0% hydrogen peroxide solution for disinfection for 3 minutes, Then, after washing twice with sterile water, insert into White + activated carbon 0.5g / L + adenine 5mg / L + sucrose 25g / L liquid medium, and induce culture at a temperature of 15°C and without light for 25 days;

[0038] b. Transfer the seeds after induction culture for 30 days into 1 / 2MS+6BA1.0mg / L+TDZ0.3mg / L+NAA0.1mg / L+activated carbon 1.0g / L medium, at a temperature of 22°C and light every day 15 hours and light intensity were cultivated for 40 days under the condition of 2000lx, and statistics had bulbs growing at the base of the Rhododendron orchid b...

Embodiment 3

[0048] The rapid cultivation method of the rhododendron orchid regeneration plant that the present embodiment provides comprises the steps:

[0049] (1) Cultivation of rhododendron orchid bulbs

[0050] a. Select seeds from ripe and freshly cracked rhododendron orchid fruits, soak the seeds in 6.0% calcium hypochlorite solution for 40 minutes, then put them into 10.0% hydrogen peroxide solution for disinfection for 6 minutes, Then, after washing with sterile water for 5 times, insert into White + activated carbon 1.0g / L + adenine 8mg / L + sucrose 30g / L liquid medium, and induce culture for 40 days at a temperature of 22°C and without light;

[0051] b. Transfer the seeds after induction culture for 30 days into 1 / 2MS+6BA3.0mg / L+TDZ0.6mg / L+NAA0.5mg / L+activated carbon 2.0g / L medium, at a temperature of 26°C and light every day 24 hours, under the condition of light intensity of 3000lx, cultivated for 60 days, statistics have 69.53% of rhododendron orchid buds to grow with bulbs ...

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Abstract

The invention discloses a quick cultivation method for regenerated plantlets of cremastra appendiculata. The quick cultivation method comprises the following steps: (1) culturing cremastra appendiculata corms; (2) selecting explants; (3) inducing adventitious buds; (4) rooting the rootless seedlings; (5) hardening seedlings. According to the invention, the corms on the cremastra appendiculata budbases are used as the explants, the adventitious buds are induced, the rooting culture for the rootless seedlings is performed and the seedlings are hardened, so that the culturing time of the regenerated plantlets of cremastra appendiculata is greatly shortened, a new method is supplied for large-scale reproduction of the regenerated plantlets of the cremastra appendiculata and the problem of shortage of present cremastra appendiculata plant resources and seedling resources can be effectively solved.

Description

technical field [0001] The invention relates to a rapid cultivation method of rhododendron orchid regenerated plants, in particular to a rapid cultivation method of rhododendron orchid regenerated plants using rhododendron orchid bulbs as explants. Background technique [0002] Rhododendron orchid [Cremastra appendiculata (D.Don.) Makion], also known as Maoji mushroom and Abacus seven, is a rare medicinal and ornamental plant of the genus Rhododendron in the family Orchidaceae. It is mainly distributed in East Asia, especially in Guizhou and Sichuan of China. For many. Use pseudobulb as medicine, taste pungent, sweet, cold in nature, slightly poisonous. It has the effects of clearing heat and detoxifying, nourishing the lungs and relieving cough, promoting blood circulation and relieving pain, reducing swelling and dispelling stagnation, etc. It can be used internally to fight liver cancer, breast cancer, uterine cancer, etc., and it can be used externally to treat sores, s...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王跃华陈燕黄鹏陈芳马涛曾宇刘欢吉礼涛唐亚铃何新友张霞杜媛唐捷王宗兵
Owner CHENGDU UNIV
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