Polysaccharide P4, separation and purification method thereof and application of polysaccharide P4 to blood-lipid lowering drug

A technology for separation and purification of polysaccharides, which is applied in the field of natural products, can solve the problems of low processing utilization level and low development and utilization rate, and achieve the effects of good physical and chemical stability, low cost and high yield

Active Publication Date: 2019-01-04
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Myrtle (Rhodomyrtus tomentosa) is also known as Gangren (Guangdong), Doumingan (Guangxi), Shidu Nianzi ("Supplement to Materia Medica"), Daomanzi ("Lingbiao Luyi"), Daonianzi ("Su Shen Liangfang") 》) etc., widely distributed in subtropical regions; dominant species in evergreen broad-leaved forest ecosystems, with a coverage of 30-60%, and a pioneer community; mainly distributed in south of the Five Ridges in my country,

Method used

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  • Polysaccharide P4, separation and purification method thereof and application of polysaccharide P4 to blood-lipid lowering drug
  • Polysaccharide P4, separation and purification method thereof and application of polysaccharide P4 to blood-lipid lowering drug
  • Polysaccharide P4, separation and purification method thereof and application of polysaccharide P4 to blood-lipid lowering drug

Examples

Experimental program
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Effect test

Embodiment 1

[0042] A method for extracting and isolating and purifying polysaccharides from myrtle fruit, comprising the following steps:

[0043] (1) Extract polysaccharides from myrtle fruit: Weigh 110g of dried myrtle fruit and crush them, pass through a 40-mesh sieve, weigh 100g of dry powder, add 1000mL of distilled water, boil in a reflux device for 4h, and take the filter residue after suction filtration Then add 1000 mL of distilled water, boil for 4 h in a reflux device, combine the supernatants, and concentrate under reduced pressure. Slowly add 4 times the volume of 95% (V / V) ethanol to the concentrated solution, and keep stirring with a glass rod to uniformly precipitate the polysaccharide, then put it in a refrigerator at 4°C for 12 hours, and then centrifuge at 5000r / min for 15 minutes to collect the precipitate. After drying, myrtle fruit crude polysaccharide is obtained;

[0044] (2)H 2 o2 Decolorization method: Weigh 20g myrtle fruit crude polysaccharide, add 100mL dist...

Embodiment 2

[0054] The myrtle fruit polysaccharide P4 obtained in Example 1 was analyzed by ultraviolet spectrum, and 1 mg polysaccharide sample was weighed to prepare a 1 mg / mL polysaccharide solution, and the ultraviolet spectrum in the range of 200-500 nm was scanned.

[0055] figure 2 It is the ultraviolet spectrogram of P4, and the results show that this component has no obvious absorption peaks at 260nm and 280nm, indicating that it does not contain protein and nucleic acid substances.

Embodiment 3

[0057] Carry out polysaccharide molecular weight analysis to the myrtle fruit polysaccharide P4 that embodiment 1 obtains, concrete experimental method is as follows:

[0058] Molecular weights were determined using gel permeation chromatography (GPC). Take 2 mg of polysaccharide sample, add 0.02 mol / L phosphate buffer solution to dissolve, prepare 2.0 mg / mL solution, filter with 0.22 μm sterile filter membrane, and take the filtrated liquid for later use. Chromatographic conditions: column temperature 35°C, 0.02mol / L phosphate buffer as mobile phase, flow rate 0.6mL / min, injection volume 20μL, Waters 2410 differential refractive index detector for detection. Prepare a series of dextran solutions with different molecular weights (700, 400, 200, 100, 50, 30, 10, 5kD) as standards, draw a standard curve, and calculate the molecular weight of the sample according to its corresponding elution volume against the standard curve .

[0059] The measured molecular weight of the myrtl...

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Abstract

The invention discloses a myrtle polysaccharide P4, a separation and purification method thereof and an application of the polysaccharide P4 to preparation of a blood-lipid lowering drug. The polysaccharide comprises, in mole percent, 10.48% of monosaccharide: ribose, 4.05% of rhamnose, 65.50% of arabinose, 5.13% of xylose, 2.39% of mannose, 4.74% of glucose and 7.71% of galactose. Experimental results indicate that the myrtle polysaccharide P4 has a certain in-vitro cholate binding capacity, colestyramine serves as a positive control, and relative binding rates of the myrtle polysaccharide P4for sodium taurocholate, sodium glycocholate and sodium cholate are 31.54%, 47.61% and 52.76% by transformation according to the binding rate of 100% of the colestyramine for cholate.

Description

technical field [0001] The invention belongs to the field of natural products (drugs), and in particular relates to a myrtle polysaccharide P4 and its separation and purification method and its application in the preparation of blood lipid-lowering drugs. Background technique [0002] Myrtle (Rhodomyrtus tomentosa) is also known as Gangren (Guangdong), Doumingan (Guangxi), Shidu Nianzi ("Supplement to Materia Medica"), Daomanzi ("Lingbiao Luyi"), Daonianzi ("Su Shen Liangfang") 》) etc., widely distributed in subtropical regions; dominant species in evergreen broad-leaved forest ecosystems, with a coverage of 30-60%, and a pioneer community; mainly distributed in south of the Five Ridges in my country, and is the main understory vegetation in southern China , the resources are very rich, most of which are born in hilly slopes, wilderness, roadsides, and wild myrtle is widely distributed, but at present its development and utilization rate is low, and the level of processing and...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P3/06
CPCA61K31/715A61P3/06C08B37/0003C08B37/006
Inventor 黄儒强王静辉王倩高林林张竞雯
Owner SOUTH CHINA NORMAL UNIVERSITY
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