Method for purifying and preparing antibacterial peptide

A technology of antimicrobial peptides and antimicrobial peptide solutions, applied in the field of purification, to achieve the effects of reducing the number of purification times and steps, shortening the purification process route, and improving purification efficiency and yield
CN109180779AActive Publication Date: 2019-01-11CHENGDU SINTANOVO BIOTECHNOLOGV CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHENGDU SINTANOVO BIOTECHNOLOGV CO LTD
Publication Date
2019-01-11

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Abstract

The invention discloses a method and device for purifying and preparing an antibacterial peptide. The method and device solve the problem that the prior art cannot guarantee a yield of 50% or more while the purity is greater than 99% in antibacterial peptide purification. The method comprises: 1, preparing a crude antibacterial peptide solution, 2, adding the crude antibacterial peptide solution into a semipermeable membrane, and orderly carrying out dialysis through a buffer and purified water to obtain an antibacterial peptide primary pure solution, 3, refining the antibacterial peptide primary pure solution through reverse phase chromatography to obtain an antibacterial peptide refined pure solution, and 4, transferring salts in the antibacterial peptide refined pure solution through anHPLC method to obtain an antibacterial peptide solution and carrying out reduced pressure concentration and drying to obtain an antibacterial peptide finished product. The method can promote the semi-permeable membrane purification and the reverse chromatography purification and realizes a yield of 50% or more while the purity is greater than 99%.
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Description

technical field

[0001] The invention relates to the field of purification, in particular to a method for purifying and preparing antimicrobial peptides. Background technique

[0002] Antimicrobial peptides are a class of polypeptides with antibacterial activity. By acting on the bacterial cell membrane, destroying its integrity and producing perforation, it enters the cell and destroys its organelles to cause metabolic disorders. Bacterial contents leak out of the cell causing bacterial death. Antimicrobial peptides not only have a good bactericidal effect on bacteria and fungi, but also have antiviral activity and promote wound healing. At the same time, the combination of antimicrobial peptide and lysozyme also has a synergistic antibacterial effect. Under the action of these two core components, a double wall-breaking and bactericidal effect on pathogenic bacteria is formed.

[0003] The antimicrobial peptide is similar to MSI-78 in foreign clinical trials, which has c...

Claims

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