A kind of enterobacteriaceae and application for preventing and treating plant root-knot nematode

A technology of enterobacteriaceae and knot nematodes, which is applied to enterobacteriaceae and application fields for the prevention and treatment of plant root-knot nematodes, can solve the problems of large waste of chemical nematicides, restrictions on the application of chemical nematicides, and destruction of soil biota, and achieve Excellent control effect, excellent control effect, effect of promoting plant growth

Active Publication Date: 2021-06-18
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the control of plant parasitic nematodes is still dominated by chemical control, but in recent years, the application of chemical nematicides is wasteful, pollutes the environment, has poor selectivity, strong extinction, and damages soil biota. The 21st century is called the century of environmental protection, and the application of some effective chemical nematicides is gradually restricted, so the research on the biological control of plant parasitic nematodes has naturally become a hot issue

Method used

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  • A kind of enterobacteriaceae and application for preventing and treating plant root-knot nematode
  • A kind of enterobacteriaceae and application for preventing and treating plant root-knot nematode
  • A kind of enterobacteriaceae and application for preventing and treating plant root-knot nematode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Preparation of Enterobacter ludwigii Sneb1987 bacterial agent

[0016] The bacterial strain of Enterobacter ludwigii Sneb1987 was inoculated on the test tube medium, and the medium formula was yeast mannitol agar (YMA), and the composition was (g / L): yeast extract 1.0; mannitol 10.0; dipotassium hydrogen phosphate 0.5; Magnesium Sulfate 0.2; Chloride 0.1; Calcium Carbonate 1.0; Agar 15g;

[0017] Then inoculate the test tube into a 250mL Erlenmeyer flask (150mL per bottle) liquid medium, the medium is YMB medium, the composition is (g / L): yeast extract 1.0; mannitol 10.0; dipotassium hydrogen phosphate 0.5; sulfuric acid Magnesium 0.2; Sodium Chloride 0.1; Calcium Carbonate 1.0; pH 6.8-7.0. The fermentation temperature is 25-28°C, the shaker speed is 150r min -1 , Fermentation time 2-3d.

[0018] Adjust the concentration of the above enterobacteria fermentation broth to 10 8 CFU / mL, made into Enterobacter inoculum, used to treat tomato seeds.

[0019] Test location...

Embodiment 2

[0026] Preparation of Enterobacter ludwigii Sneb1987 bacterial agent

[0027] The bacterial strain of Enterobacter ludwigii Sneb1987 was inoculated on the test tube culture medium, and the culture medium formula is Yeast Mannitol Agar (YMA), and composition is (g / L): yeast extract 1.0; Mannitol

[0028] 10.0; Dipotassium hydrogen phosphate 0.5; Magnesium sulfate 0.2; Chloride 0.1; Calcium carbonate 1.0; Agar 15g;

[0029] Then the strains in the test tube were connected to the shaker flask for fermentation, and in the liquid medium of 250mL Erlenmeyer flask (150mL per bottle), the medium was YMB medium, and the composition was (g / L): yeast extract 1.0; mannitol

[0030] 10.0; dipotassium hydrogen phosphate 0.5; magnesium sulfate 0.2; sodium chloride 0.1; calcium carbonate 1.0; pH 7.0. Fermentation temperature is 28°C, shaker speed is 150r min -1 , Fermentation time 2-3d, adjust the concentration of bacteria solution to 10 7 CFU / mL, made Enterobacter bacteria agent.

[0031...

Embodiment 3

[0042] Embodiment 3: Enterobacter ludwigii (Enterobacter ludwigii) Sneb1987 inoculant agent controls root-knot nematode pot plant effect test

[0043] In order to verify the stability of its induced resistance, the research on the control effect of potted plants is hereby carried out. The experiment was carried out in the greenhouse of Shenyang Agricultural University for the effect test of potted plants. The uncoated tomato seeds and the tomato seeds coated with YMB liquid culture solution were used as controls, and the experiment was repeated twice.

[0044] Enterobacteriaceae bacterial agent preparation method is with embodiment 1;

[0045] Test tomato variety is with embodiment 1;

[0046] Seed treatment method is with embodiment 1;

[0047] Nematodes for testing: collected from tomato roots with severe disease in the greenhouses of Yingpan Village, Liqianhu Town, Tieling City, Liaoning Province, identified as Meloidogyne incognita, picked full and mature oocysts with tw...

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Abstract

The invention relates to an enterobacter for preventing and treating root-knot nematode and its application. The preservation unit of the enterobacter is the China General Microorganism Culture Preservation Management Center, the preservation number is CGMCC No.16336, and the classification is named Ludwig enterobacterium Bacillus ( Enterobacter ludwigii ); the bacterial agent of the enterobacteriaceae has a control effect on nematodes, and has the purposes of improving plant resistance to nematodes and promoting plant growth; the bacterial agent of enterobacteriaceae proposed by the present invention has excellent control effect on plant root-knot nematodes, and simultaneously It can promote plant growth, improve plant resistance to nematodes, and provide new resources for the development of new seed treatment agents and inducers; the mixed use of Enterobacteriaceae and traditional nematicides has excellent control effect on nematodes and can reduce chemical agents The amount of application can reduce pesticide residues, which is of environmental protection significance.

Description

technical field [0001] The invention belongs to the field of biological pesticides, and in particular relates to an enterobacter for preventing and treating plant root-knot nematodes and its application. Background technique [0002] Plant parasitic nematodes occur and cause damage in many countries and regions in the world, and there are various types. On a global scale, the occurrence and harm of plant parasitic nematodes have become increasingly serious with the development of agriculture and forestry and the changes in farming and cultivation systems. According to Esser statistics, 207 genera and 4832 species of plant parasitic nematodes have been reported to have been found in the world until 1990. Plant-parasitic nematodes are estimated to cause an annual loss of 12.3% of the world's major crops, exceeding US$ 100 billion, while actual losses far exceed estimates, citing the fact that damage caused by many nematodes is limited by the absence of field-visible symptoms o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P5/00A01P21/00C12R1/01
CPCA01N63/00C12N1/20C12N1/205C12R2001/01Y02A50/30
Inventor 陈立杰王帅段玉玺朱晓峰刘晓宇范海燕王媛媛
Owner SHENYANG AGRI UNIV
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