A kind of enterobacteriaceae and application for preventing and treating plant root-knot nematode
A technology of enterobacteriaceae and knot nematodes, which is applied to enterobacteriaceae and application fields for the prevention and treatment of plant root-knot nematodes, can solve the problems of large waste of chemical nematicides, restrictions on the application of chemical nematicides, and destruction of soil biota, and achieve Excellent control effect, excellent control effect, effect of promoting plant growth
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Embodiment 1
[0015] Preparation of Enterobacter ludwigii Sneb1987 bacterial agent
[0016] The bacterial strain of Enterobacter ludwigii Sneb1987 was inoculated on the test tube medium, and the medium formula was yeast mannitol agar (YMA), and the composition was (g / L): yeast extract 1.0; mannitol 10.0; dipotassium hydrogen phosphate 0.5; Magnesium Sulfate 0.2; Chloride 0.1; Calcium Carbonate 1.0; Agar 15g;
[0017] Then inoculate the test tube into a 250mL Erlenmeyer flask (150mL per bottle) liquid medium, the medium is YMB medium, the composition is (g / L): yeast extract 1.0; mannitol 10.0; dipotassium hydrogen phosphate 0.5; sulfuric acid Magnesium 0.2; Sodium Chloride 0.1; Calcium Carbonate 1.0; pH 6.8-7.0. The fermentation temperature is 25-28°C, the shaker speed is 150r min -1 , Fermentation time 2-3d.
[0018] Adjust the concentration of the above enterobacteria fermentation broth to 10 8 CFU / mL, made into Enterobacter inoculum, used to treat tomato seeds.
[0019] Test location...
Embodiment 2
[0026] Preparation of Enterobacter ludwigii Sneb1987 bacterial agent
[0027] The bacterial strain of Enterobacter ludwigii Sneb1987 was inoculated on the test tube culture medium, and the culture medium formula is Yeast Mannitol Agar (YMA), and composition is (g / L): yeast extract 1.0; Mannitol
[0028] 10.0; Dipotassium hydrogen phosphate 0.5; Magnesium sulfate 0.2; Chloride 0.1; Calcium carbonate 1.0; Agar 15g;
[0029] Then the strains in the test tube were connected to the shaker flask for fermentation, and in the liquid medium of 250mL Erlenmeyer flask (150mL per bottle), the medium was YMB medium, and the composition was (g / L): yeast extract 1.0; mannitol
[0030] 10.0; dipotassium hydrogen phosphate 0.5; magnesium sulfate 0.2; sodium chloride 0.1; calcium carbonate 1.0; pH 7.0. Fermentation temperature is 28°C, shaker speed is 150r min -1 , Fermentation time 2-3d, adjust the concentration of bacteria solution to 10 7 CFU / mL, made Enterobacter bacteria agent.
[0031...
Embodiment 3
[0042] Embodiment 3: Enterobacter ludwigii (Enterobacter ludwigii) Sneb1987 inoculant agent controls root-knot nematode pot plant effect test
[0043] In order to verify the stability of its induced resistance, the research on the control effect of potted plants is hereby carried out. The experiment was carried out in the greenhouse of Shenyang Agricultural University for the effect test of potted plants. The uncoated tomato seeds and the tomato seeds coated with YMB liquid culture solution were used as controls, and the experiment was repeated twice.
[0044] Enterobacteriaceae bacterial agent preparation method is with embodiment 1;
[0045] Test tomato variety is with embodiment 1;
[0046] Seed treatment method is with embodiment 1;
[0047] Nematodes for testing: collected from tomato roots with severe disease in the greenhouses of Yingpan Village, Liqianhu Town, Tieling City, Liaoning Province, identified as Meloidogyne incognita, picked full and mature oocysts with tw...
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