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A kind of culture medium for separating yeast from fermented grains and its preparation method

A technology for separating medium and yeast, which is applied in the direction of separating microorganisms and fungi, can solve the problems of adding Daqu-wine grains extract, etc., and achieve the effects of system separation, good yeast growth environment, and promotion of separation

Active Publication Date: 2021-11-12
KWEICHOW MOUTAI COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report about adding Daqu-wine grains extract to the medium for the isolation of yeasts from fermented grains

Method used

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  • A kind of culture medium for separating yeast from fermented grains and its preparation method
  • A kind of culture medium for separating yeast from fermented grains and its preparation method
  • A kind of culture medium for separating yeast from fermented grains and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1 yeast isolation medium formula and preparation method thereof

[0056] Components and contents of the medium in table 1

[0057] components Medium 1 Medium 2 Medium 3 Medium 4 Medium 5 Yeast extract 5g 5g 5g 7g 7g Peptone 10g 10g 10g 10g 15g glucose 10g 10g 15g 20g 20g agar 18g 18g 20g 20g 20g Extract 100mL 150mL 200mL 200mL 200mL

[0058] Note: The pH of the medium is natural.

[0059] 1. The preparation method of the extract is:

[0060] (1) Weigh and mix the Daqu and the fermented grains according to the ratio of 1:5;

[0061] (2) Add pure water to the mixture of Daqu-wine grains and water according to the mass ratio of 1:7 and boil for 10-15min;

[0062] (3) After cooling, filter with gauze to obtain the filtrate for later use.

[0063] 2. Preparation method of yeast isolation medium:

[0064] (1) Weigh each component according to the formula in Table 1, add yeast powder...

Embodiment 2

[0073] The effect of the addition of embodiment 2 extract on the separation of yeast in fermented grains

[0074] In order to evaluate the influence of the addition of the extract in the culture medium of the present invention on the growth of yeast, the present invention adopts the plate dilution method to make a series of diluted bacterial liquids from the fermented grains, and spread them on the culture medium shown in Table 3 respectively for cultivation , each group has three dilution gradients, which is 10 -3 、10 -4 、10 -5 (dilution factor).

[0075] Components and contents of the medium in table 3

[0076] components Medium 6 Medium 7 Medium 8 comparison group Yeast extract 5.0g 5.0g 5.0g 5.0g Peptone 10.0g 10.0g 10.0g 10.0g glucose 10.0g 10.0g 10.0g 10.0g agar 20.0g 20.0g 20.0g 20.0g Extract 700mL 500mL 200mL 0

[0077] After culturing for 48 hours, select a dilution gradient of 10 -4 For compar...

Embodiment 3

[0090] Example 3 The effect of culture medium pH on the separation of yeast in the accumulated fermented grains sample

[0091] In order to evaluate the different influences of the pH of the culture medium of the present invention on the separation of yeast, the present invention adopts the plate dilution method to make a series of diluted bacterial liquids from the accumulation of fermented grains, and spread them on the culture medium shown in Table 6 respectively for cultivation. There were 3 dilution gradients of the bacterial solution inoculated in the culture medium of the group, which was 10 -4 、10 -5 、10 -6 . Except for the difference in pH, the other components, content and preparation method of the medium are the same as those of medium 8 in Example 2.

[0092] Note: The "pH natural" means that the pH of the medium has not been adjusted by adding an acid solution.

[0093] "Adjusted pH" means that the pH of the medium is adjusted by adding an acid solution (hydro...

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Abstract

The invention relates to a yeast isolation medium in the technical field of microbial fermentation engineering, in particular to a yeast isolation medium in fermented grains and a preparation method thereof. Disclosed is a culture medium for separating yeast from fermented grains. The formula of the culture medium includes the following components: yeast powder 5-10g / L, peptone 10-20g / L, glucose 5-20g / L, agar 15-22g / L, Daqu-wine grain extract 100-800mL / L, the balance is water. According to the fermentation characteristics of fermented grains in different periods, adjusting the pH of the medium appropriately is beneficial to the systematic separation of various yeasts in the brewing process. The culture medium of the invention has the advantages of rich composition, strong pertinence and wide screening range.

Description

technical field [0001] The invention relates to a yeast isolation medium in the technical field of microbial fermentation engineering, in particular to a yeast isolation medium in fermented grains and a preparation method thereof. Background technique [0002] Yeasts widely exist in koji making, stacking, cellars, air, and drying halls, and are important microorganisms in the production process of liquor. According to their functions, they can be divided into wine-producing functional bacteria and aroma-producing functional bacteria. Their growth and metabolism have an important impact on the yield and flavor of liquor. In order to study the mechanism of action of different yeasts in liquor production, it is first necessary to conduct a comprehensive isolation and screening of yeasts. [0003] The existing medium for isolating yeast includes yeast extract powder peptone glucose medium (YPD medium), potato dextrose agar medium (PDA medium), WL nutrient agar medium (WL medium...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12N1/02
CPCC12N1/02C12N1/16
Inventor 袁颉杨帆杨玉波曾祥炼王和玉王莉白逢彦尉洪涛
Owner KWEICHOW MOUTAI COMPANY
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