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Collagen membrane for adjuvant treatment of peripheral nerve injury, and preparation method and use thereof

A technology of peripheral nerve injury and collagen membrane, applied in the field of medical devices, can solve problems such as unfavorable nerve repair and tension generated by the broken end of peripheral nerve, and achieve better effect and promote recovery.

Active Publication Date: 2019-01-25
HARBIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the problem that classic epineurium and perineurium sutures cause tension on the stumps of peripheral nerves, which is not conducive to nerve repair, and assist peripheral nerve fusion agents to overcome the need for peripheral nerve damage in the prior art. The disadvantage of nerve cell regeneration is to shift the center of treatment to the early stage of injury, repair damaged nerve fiber bundles through peripheral nerve fusion, restore the structural integrity and electrical conduction function of nerve cells, and restore nerve function as quickly as possible in the early stage of injury

Method used

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  • Collagen membrane for adjuvant treatment of peripheral nerve injury, and preparation method and use thereof
  • Collagen membrane for adjuvant treatment of peripheral nerve injury, and preparation method and use thereof
  • Collagen membrane for adjuvant treatment of peripheral nerve injury, and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Preparation of Collagen Film for Adjuvant Treatment of Peripheral Nerve Injury

[0036] 1. Solution preparation

[0037] 1w / w% collagen aqueous solution: Weigh 1g of soluble collagen powder and dissolve it in 99ml of distilled water.

[0038] Dilute acetic acid solution of chitosan with a concentration of 2w / w%: Weigh 1.0g of glacial acetic acid and dissolve it in 99ml of distilled water to obtain a dilute acetic acid solution with a concentration of 1w / w%; weigh 1.0g of chitosan and dissolve it in 49.0ml to prepare Dilute acetic acid solution, that is.

[0039] 5w / w% polyvinyl alcohol aqueous solution: weigh 5g of polyvinyl alcohol and dissolve it in 95ml of distilled water.

[0040] 0.000001w / w% aqueous solution of nerve growth factor: Weigh 0.01g of nerve growth factor and dissolve in 100ml of distilled water to obtain a 0.01w / w% aqueous solution of nerve growth factor, then dilute to 0.000001w / w% concentration.

[0041] B12 aqueous solution with a conce...

Embodiment 2

[0048] Example 2 Preparation of Collagen Film for Adjuvant Treatment of Peripheral Nerve Injury

[0049] 1. Solution preparation

[0050] With embodiment 1.

[0051] 2. Preparation of Collagen Membrane

[0052] (1) Take each raw material according to the following weight percentages:

[0053] 1w / w% collagen aqueous solution 41%, 2w / w% chitosan dilute acetic acid solution 15%, 5w / w% polyvinyl alcohol aqueous solution 20%, 0.000001w / w% nerve growth Factor aqueous solution 10%, 10w / w% concentration of B12 aqueous solution 7%, polyethylene glycol 4% and concentration of 0.25w / w% glutaraldehyde aqueous solution 3%;

[0054] (2) Collagen protein aqueous solution, dilute acetic acid solution of chitosan, polyvinyl alcohol aqueous solution, nerve growth factor aqueous solution, B12 aqueous solution and polyethylene glycol are blended, fully stirred evenly, room temperature standstill defoaming;

[0055] (3) Add glutaraldehyde aqueous solution to the blend solution in step (2), mix...

Embodiment 3

[0056] Example 3 Preparation of Collagen Film for Adjuvant Treatment of Peripheral Nerve Injury

[0057] 1. Solution preparation

[0058] With embodiment 1.

[0059] 2. Preparation of Collagen Membrane

[0060] (1) Take each raw material according to the following weight percentages:

[0061] 1w / w% collagen aqueous solution 48%, 2w / w% chitosan dilute acetic acid solution 8%, 5w / w% polyvinyl alcohol aqueous solution 24%, 0.000001w / w% nerve growth Factor aqueous solution 8%, 10w / w% concentration of B12 aqueous solution 5%, polyethylene glycol 600 5% and concentration of 0.25w / w% glutaraldehyde aqueous solution 2%;

[0062] (2) Collagen protein aqueous solution, dilute acetic acid solution of chitosan, polyvinyl alcohol aqueous solution, nerve growth factor aqueous solution, B12 aqueous solution and polyethylene glycol are blended, fully stirred evenly, room temperature standstill defoaming;

[0063] (3) Add glutaraldehyde aqueous solution to the blend solution in step (2), m...

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Abstract

The invention discloses a collagen film for auxiliary treatment of peripheral nerve injury and a preparation method and application thereof. The collagen membrane is obtained by crosslinking collagenaqueous solution, chitosan dilute acetic acid solution, polyvinyl alcohol aqueous solution, nerve growth factor aqueous solution, B12 aqueous solution and polyethylene glycol by glutaraldehyde. At present, the treatment of peripheral nerve is limited to the microsurgical anastomosis of neurotrophic drug support, this method will produce certain tension on the peripheral nerve stump, which is not conducive to the repair of the stump. The invention can overcome the problem that the peripheral nerve stump produces tension and the degradation of the collagen membrane can promote the recovery of the peripheral nerve at the early recovery stage, it can recover its basic motor function in a short time, and it is better than single epineurial suture or epineurial suture combined with nerve fusionagent.

Description

technical field [0001] The present invention relates to a collagen film for auxiliary treatment of peripheral nerve injury and its preparation method and application, in particular to a collagen film for auxiliary nerve fusion agent treatment of peripheral nerve injury and its preparation method and application. The invention belongs to the technical field of medical instruments. Background technique [0002] The current study believes that after nerve injury, the axon and myelin sheath of the nerve fibers at the distal end of the injury plane are destroyed, the connection dispute between the innervated target organ and neurons, and the entire nerve pathway collapses. This series of reactions is called This is called Wallerian degeneration. Early after injury, the activation and release of proteases in nerve cell somata degenerates the distal parts of the nerve. As the cell membrane dissolves, the cytoskeleton begins to disintegrate. The proximal nerve becomes edematous d...

Claims

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Application Information

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IPC IPC(8): A61K38/39A61K9/70A61P25/02A61K38/18A61K35/28A61K35/545A61K36/537A61K35/30A61K33/44A61K31/765
CPCA61K38/185A61K38/39A61K47/42A61P25/02A61K9/7007A61K31/765A61K33/44A61K35/28A61K35/30A61K35/545A61K36/537A61K2300/00
Inventor 任晓平任帅
Owner HARBIN MEDICAL UNIVERSITY
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