Use of nucleosome-transcription factor complexes for cancer detection
A technology of transcription factors and nucleosomes, applied in the field of primary cancer in subjects, can solve laborious, expensive and complicated problems
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Embodiment 1
[0212] Serum samples were obtained from 1 healthy subject, 2 subjects with breast cancer, and 2 subjects with urothelial bladder cancer (transitional cell carcinoma known to give positive results in the GATA 3 cancer tissue expression test). The tester gets it. A commercially available 96-well research-only ELISA kit for GATA 3 containing solid-phase GATA 3 transcription factor antibodies coated to microtiter wells was purchased. GATA 3, a tissue-specific transcription factor expressed in all or most biopsy samples from breast cancer, is also positive in transitional cell carcinoma and cutaneous adnexal tumors, but in most samples from other carcinomas Not positive. The solid-phase GATA 3 antibody was used with a biotinylated anti-nucleosome antibody in a double antibody ELISA for cell-free nucleosome-binding GATA3 as follows: serum samples (50 μl) and assay buffer (50 μl) The wells coated with anti-GATA 3 antibody were added and incubated for 2.5 hours at room temperature (...
Embodiment 2
[0215] Serum samples were taken from 2 subjects with thyroid cancer, 3 subjects with lung cancer and 1 healthy subject. A commercially available 96-well research-only ELISA kit for TTF-1 containing solid phase TTF-1 (Thyroid Transcription Factor 1 ) antibody coated to microtiter wells was purchased. TTF-1 is a tissue-specific transcription factor expressed in all or most samples of biopsy material from thyroid and lung cancers, but not in most samples from other cancers. The solid-phase TTF-1 antibody was used in a double antibody ELISA for cell-free nucleosome-binding TTF-1 as described in Example 1 with a biotinylated anti-nucleosome antibody.
[0216] Higher ELISA signals and higher levels of circulating cell-free nucleosome-TTF-1 adducts were observed for samples obtained from thyroid cancer patients than from healthy subjects or negative controls. Levels of circulating cell-free nucleosome-TTF-1 adducts are slightly elevated in the blood of lung cancer patients. result ...
Embodiment 3
[0220] Serum samples were taken from 3 healthy subjects, 1 subject diagnosed with thyroid cancer, 3 subjects with lung cancer, 3 subjects with pancreatic cancer, 6 subjects with colorectal cancer subjects (3 with colon cancer, 3 with rectal cancer) and 3 subjects with breast cancer. A commercially available 96-well research-only ELISA kit for CDX2 containing a solid-phase CDX2 (tailed homeobox transcription factor-2) antibody coated to microtiter wells was purchased. CDX2 is a tissue-specific transcription factor expressed in all or most samples of biopsy material from colorectal cancer, but not in most samples from other cancers. The solid-phase CDX2 antibody was used in a double antibody ELISA for cell-free nucleosome-binding CDX2 as described in Example 1 with a biotinylated anti-nucleosome antibody.
[0221] Higher ELISA signal and higher levels of circulating cell-free nucleosome-CDX2 adducts were observed for samples obtained from colorectal cancer patients than from he...
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