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Method for detecting paclitaxel in taxus chinensis extract

A technology of yew extract and detection method, applied in the field of detection of paclitaxel in yew extract, can solve problems such as no solution

Inactive Publication Date: 2019-02-12
东阳市杰迩威生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be used as an additive of anti-tumor Chinese patent medicine, which can treat tumors and relieve the pain of patients; however, there has been no way to solve the ingredients of the yew extract and how to detect the ingredients and content of the yew extract

Method used

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  • Method for detecting paclitaxel in taxus chinensis extract
  • Method for detecting paclitaxel in taxus chinensis extract
  • Method for detecting paclitaxel in taxus chinensis extract

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Embodiment Construction

[0020] The present invention will be described in detail below in conjunction with accompanying drawing:

[0021] The present invention uses yew (twigs, roots, bark, trunks, excluding leaves) as raw materials, and is made of flavonoids, phenols, and terpenes through specific processing techniques such as aqueous ethanol extraction, concentration, extraction, drying, and crushing. , paclitaxel as the main index component of the plant extract (see the invention patent, patent number 200910152866.3).

[0022] The detection method of paclitaxel in this yew extract, concrete steps are as follows:

[0023] 1 reagent

[0024] Paclitaxel standard substance (Chengdu Master Biological Technology Co., Ltd.), methanol, acetonitrile (chromatographically pure, Fisher Company), Wahaha purified water

[0025] 2 instruments

[0026] Agilent Technologies1200 Series HPLC, ultrasonic extractor, analytical balance, rotary evaporator

[0027] 3 analysis steps

[0028] 3.1 Chromatographic condi...

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Abstract

The invention discloses a method fof detecting the paclitaxel in taxus chinensis extract, comprising the following steps of: (1.1) preparing the control solution; (1.2) drawing the standard curve; (1.3) preparing the test solution, so as to accurately weigh 0.25 g of the taxus chinensis extract, ultrasound with 60% ethanol for 10 min and dissolve, constant volume to 25mL volumetric flask, suck thesolution through a 0.45 [mu]m of microfiltration membrane to obtain the test solution; (1.4) measuring the sample, so as to take the test solution according to the content of paclitaxel in the sample, measure according to a chromatographic condition, inject 20L of the sample, and measure the peak area; (1.5) calculating the result, so as to calculate the paclitaxel content corresponding to the peak area of the sample according to the standard curve, and calculating the paclitaxel content according to the following formula. The method for detecting the paclitaxel in taxus chinensis extract hasthe advantages of: detecting and analyzing the quality standard related items such as determination of the total paclitaxel and measurement of the component content, and finally forming the completean enterprise standard of the attenuated taxus chinensis extract.

Description

technical field [0001] The invention relates to the field of yew, and mainly relates to a method for detecting paclitaxel in a yew extract. Background technique [0002] On September 18, 2009, the applicant applied for a process named: Taxus chinensis non-toxic extract, the patent number is: 200910152866.3, which is used as an additive of health products, which can allow people to prevent tumors in advance. It can be used as an additive of anti-tumor Chinese patent medicine, can treat tumors, and can alleviate the pain of patients; but the composition of the yew extract and how to detect the composition and content of the yew extract have not been resolved. Contents of the invention [0003] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a method for detecting paclitaxel in the yew extract. [0004] The purpose of the present invention is accomplished through the following technical solutions. The detection method of pac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 应成杰甘礼社诸凯凯应当冲
Owner 东阳市杰迩威生物科技有限公司