Anti-tumor experimental animal model and construction method thereof

Abstract

The invention belongs to the technical field of vertebrate breeding, and discloses an anti-tumor experimental animal model and a construction method thereof. The right armpits of mice or rats for experiments are taken for subcutaneous vaccination of a mouse or rat tumor sterile diluent according to the sterile operation, and weighing and grouping are performed after 24 hours; weighing is performedafter 24 hours, random grouping is performed, 10 mice or rats fall into one group, 20 mg / kgi.p of CTX is applied every day, and intragastric administration is performed on the other groups; a constant amount of distilled water is given to a model control group, the equal amount of normal saline is inoculated on normal animals which are animals with no inoculated tumor as ten animals as a fake inoculation group, the constant volume of distilled water is fed every day to the normal animals as a normal control group, and the water is fed every day for 7 days. According to the animal experimentalmodel, a tumor sterile diluent is directly injected into experimental animals under the sterile condition, tumors grow inside the animals, the verification process is short, and by weighing the tumorweight and calculating the inhibition rate, the verification effect is convictive.

Description

technical field [0001] The invention belongs to the technical field of cultured vertebrates, and in particular relates to an anti-tumor experimental animal model and a construction method thereof. Background technique [0002] At present, the commonly used prior art in the industry is as follows: cancer is a common and frequently-occurring disease in human beings, and it has become a synonym for death due to its high mortality rate. Cancer is a serious threat to human health and life. All countries in the world have invested a lot of manpower and material resources to attack cancer in an all-round way. Studies have shown that traditional Chinese medicine not only has a good curative effect on cancer treatment, but also can reduce side effects and disease recurrence rate, can effectively prolong the survival period of patients and improve the quality of life of patients, and stabilize or shrink tumors to a certain extent. Therefore, traditional Chinese medicine and the combi...

AI Technical Summary

Problems solved by technology

[0003] To sum up, the problems existing in the prior art are: traditional anti-tumor animal models need to culture a variety of tumor cells, and then transplant them into animals to allow them to spread; group verification by injecting anti-tumor drugs takes a long time. It takes a long time, and the experimental animals will die due to various reasons during the verification process, which makes the verification effect not convincing

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