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Cultivation method of cordyceps sinensis

A cultivation method and technology of Cordyceps, applied in botany equipment and methods, cultivation, plant cultivation, etc., can solve the problems of slow cultivation speed and long growth time of mycelium, and achieve the effect of significant difference and growth promotion

Inactive Publication Date: 2019-02-19
泰安皇草生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] But the cultivation method of existing Cordyceps also exists mycelia growth time is long, the defect that cultivation speed is slow

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for cultivating Cordyceps, the method for cultivating comprises the following steps:

[0033] 1) Culture medium preparation: select high-quality green silkworm chrysalis, soak it in the culture solution for 35 minutes, and then put it into a glass culture bottle to obtain the culture medium;

[0034] Wherein, the formula ratio of described culture solution is: potato 80g, glucose 30g, milk powder 15g, potassium dihydrogen phosphate 7g, magnesium sulfate 3g, add water and dilute to 1000mL;

[0035] 2) Sterilization: Seal the culture bottle and put it into an autoclave for sterilization, heat up to 100°C, close the exhaust valve, keep it for 1 hour when the pressure of the autoclave is 1.5 bar, take it out, and cool it down;

[0036] 3) Inoculation: under aseptic conditions, spray the liquid bacteria evenly on the surface of the culture medium with an inoculation gun;

[0037] 4) Management:

[0038] ①Move the culture bottle with the liquid bacteria into the cu...

Embodiment 2

[0042] A method for cultivating Cordyceps, the method for cultivating comprises the following steps:

[0043] 1) Culture medium preparation: select high-quality green silkworm chrysalis, soak it in the culture solution for 35 minutes, and then put it into a glass culture bottle to obtain the culture medium;

[0044] Wherein, the formula ratio of described culture solution is: potato 100g, glucose 20g, milk powder 25g, potassium dihydrogen phosphate 5g, magnesium sulfate 6g, add water and dilute to 1000mL;

[0045] 2) Sterilization: Seal the culture bottle and put it into an autoclave for sterilization, heat up to 100°C, close the exhaust valve, keep it for 1 hour when the pressure of the autoclave is 1.4 bar, take it out, and cool it down;

[0046] 3) Inoculation: under aseptic conditions, spray the liquid bacteria evenly on the surface of the culture medium with an inoculation gun;

[0047] 4) Management:

[0048] ①Move the culture bottle with the liquid bacteria into the c...

Embodiment 3

[0052] A method for cultivating Cordyceps, the method for cultivating comprises the following steps:

[0053] 1) Culture medium preparation: select high-quality green silkworm chrysalis, soak it in the culture solution for 35 minutes, and then put it into a glass culture bottle to obtain the culture medium;

[0054] Wherein, the recipe ratio of the culture solution is: 90g of potatoes, 25g of glucose, 20g of milk powder, 6g of potassium dihydrogen phosphate, 5g of magnesium sulfate, and add water to make up to 1000mL;

[0055] 2) Sterilization: Seal the culture bottle and put it into an autoclave for sterilization, heat up to 100°C, close the exhaust valve, keep it for 1 hour when the pressure of the autoclave is 1.4 bar, take it out, and cool it down;

[0056] 3) Inoculation: under aseptic conditions, spray the liquid bacteria evenly on the surface of the culture medium with an inoculation gun;

[0057] 4) Management:

[0058] ①Move the culture bottle with the liquid bacteria...

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PUM

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Abstract

The invention relates to the technical field of cordyceps sinensis, in particular to a cultivation method of cordyceps sinensis. The cultivation method comprises the following steps that 1, a culturemedium is prepared, wherein high-quality green silkworm chrysalises are selected, soaked in a culture solution for 35 min and then put into a glass culture bottle to obtain the culture medium; the culture solution is prepared from 80-100 g of potatoes, 20-30 g of glucose, 15-25 g of milk powder, 5-7 g of monopotassium phosphate and 3-6 g of magnesium sulfate, and water is added for constant volumeat 1000 mL; 2, sterilization is carried out; 3, inoculation is carried out; 4, the culture bottle inoculated with liquid strains is transferred into a culture room for lucifugal culture, the temperature is controlled at 20 DEG C, the pH value is 4-9, standing is carried out for 6-8 h, then the culture bottle is placed in an oscillation cabinet under the conditions that the temperature is 25 DEG Cand the rotation speed is 160 r / min to be cultured for 10-15 min, subjected to magnetic treatment for 40 min under the condition of certain magnetic flux after being taken out and then cultured underthe conditions that the temperature is 20 DEG C and the pH value is 6-7; 5, harvesting is carried out. The cultivation method of cordyceps sinensis effectively makes up the defects that an existing cultivation method of cordyceps sinensis has long growth time of hyphae and slow cultivation speed.

Description

technical field [0001] The invention relates to the technical field of Cordyceps, in particular to a method for cultivating Cordyceps. Background technique [0002] Cordyceps sinensis is the abbreviation of Cordyceps sinensis, which belongs to a kind of Cordyceps sinensis. It is a fungus of the family Ergot, and it is a complex of parasitoids and larval corpses that parasitize on the larvae of bat moths. [0003] It has various effects such as regulating immune system function, anti-tumor, and anti-fatigue. Its cutting-edge theory, unique formula, and exclusive positioning are at the forefront. It is mainly distributed in China's Tibet, Qinghai Province, Sichuan and Yunnan, and this fungus exists in parts of South Korea. [0004] Domestic and foreign studies on the species that have induced Cordyceps fruiting bodies believe that the edible and medicinal value of silkworm chrysalis Cordyceps sinensis is comparable to that of traditional Cordyceps sinensis. It is an ideal su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20A01G18/30A01G18/50A01G18/69
CPCA01G18/00A01G18/20A01G18/30A01G18/50A01G18/69
Inventor 庄煇宏陈春莲
Owner 泰安皇草生物医药科技有限公司