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Detection method of goat MYLK4 gene CNV marker and application thereof

A goat and gene technology is applied in the field of genetics and breeding to achieve the effect of accurate and reliable method and easy operation.

Active Publication Date: 2019-02-19
NORTHWEST A & F UNIV
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Problems solved by technology

Regarding the function of the MYLK4 gene, according to studies on humans and mice, it has been found that it has important effects on the regulation of cell growth and disease onset, but there are few reports on goats

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  • Detection method of goat MYLK4 gene CNV marker and application thereof
  • Detection method of goat MYLK4 gene CNV marker and application thereof
  • Detection method of goat MYLK4 gene CNV marker and application thereof

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Embodiment Construction

[0031] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.

[0032] The present invention uses real-time fluorescent quantitative PCR to detect the copy number variation of the goat MYLK4 gene and is used for molecular breeding, comprising the following steps:

[0033] (1) Use the NCBI database to find the MYLK4 gene sequence, then use the Primer5 software to design primers, and use ordinary PCR to detect the primers;

[0034] (2) Use real-time fluorescent quantitative PCR (qPCR) technology to detect the copy number variation of candidate sites in the population, and screen out CNV markers related to goat growth traits;

[0035] (3) Use SPSS 23.0 software to conduct association analysis between the type of copy number variation and the growth traits of goats;

[0036] (4) Breeding goats with excellent growth traits according to the type of copy number variation.

[0037] 1. Goat sample collection

[...

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Abstract

The invention discloses a detection method of a goat MYLK4 gene CNV marker and application thereof. The detection method is based on the real-time fluorescence quantification PCR, a goat genome DNA serves as a template, copy number variable regions of goat MYLK4 genes are amplified, then -delta delta Ct calculation is applied, and copy number variation types of individuals are judged according toresults. According to the detection method of a goat MYLK4 gene CNV marker and application thereof, CNV types and growth characteristics of the individuals are used for association analysis; the theoretical basis is laid for establishing the relationship between MYLK4 gene copy number variations and the goat growth characteristics; the detection method is simple, convenient and fast and convenientto apply and popularize, and goat populations with excellent germplasm resources can be quickly established.

Description

technical field [0001] The invention belongs to the field of genetic breeding, in particular to a method for detecting MYLK4 gene copy number variation (CNV) markers and its application in molecular breeding for improving growth traits of goats. Background technique [0002] With the rapid development of related disciplines such as genomics and bioinformatics, the theory and technology of animal genetics and breeding have also undergone major changes, that is, the traditional conventional phenotypic breeding has gradually shifted to molecular breeding. At present, research on molecular breeding is mainly focused on marker-assisted selection (MAS), which is a technology that selects genetic resources or breeding materials through DNA molecular markers, and improves the comprehensive traits of livestock and poultry. In livestock and poultry breeding, through the selection of DNA markers that are closely related to growth traits and quantitative traits, the purpose of early sel...

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Application Information

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IPC IPC(8): C12Q1/6858C12Q1/6888
CPCC12Q1/6858C12Q1/6888C12Q2600/124C12Q2600/156C12Q2531/113C12Q2537/16
Inventor 黄永震师书玥李丽娟徐琳娜张子敬王献伟贺花陈宏雷初朝胡沈荣
Owner NORTHWEST A & F UNIV
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