Cassia seed control extract, preparing method and application thereof

A cassia seed and extract technology, which is applied in the field of cassia seed naphthopyrone reference extract and its preparation, can solve the problems of complex process, time-consuming and laborious, high cost, etc., and achieve good reproducibility, simple process and stable content Effect

Active Publication Date: 2019-02-19
BEIJING UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The process is very complicated, high cost, small amount of single sample preparation, time-consuming and laborious, poor operability, and not suitable for large-scale production

Method used

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  • Cassia seed control extract, preparing method and application thereof
  • Cassia seed control extract, preparing method and application thereof
  • Cassia seed control extract, preparing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0030] The preparation method of Semen Cassiae reference extract of the present invention comprises the steps:

[0031] (1) Put the cassia seed ethanol extract on a macroporous adsorption resin column, carry out gradient elution with the first eluent and the second eluent successively, obtain the first eluent and the second eluent respectively, and collect the The second eluate was dried to obtain semi-preparative fraction I;

[0032] Wherein, the first eluent is 15-30vol% ethanol aqueous solution, and the dosage is 2-4 times the volume of the macroporous adsorption resin column; the second eluent is 40-60vol% ethanol aqueous solution, and the dosage is 4 to 6 times the volume of the macroporous adsorption resin column;

[0033] (2) Mix the semi-prepared component I with the blank silica gel for sample mixing according to the ratio of 1:1.8 to 2.4 by weight to obtain the sample mixing silica gel;

[0034] (3) Pack the column with blank silica gel dry method for chromatograph...

preparation example 1

[0058] Using 30 vol% ethanol aqueous solution as a solvent to heat and reflux the cassia medicinal material for 3 times, the amount of solvent used each time is 10 times that of the cassia medicinal material, and the extraction time is 2 hours each time to obtain an alcohol extract; Dry to obtain the cassia seed alcohol extract. Add water to disperse the cassia seed ethanol extract to 0.05 g / ml to obtain a sample solution.

[0059] Pack the AB-8 macroporous adsorption resin into a column to obtain an AB-8 macroporous adsorption resin column with a diameter-to-height ratio of 1:8.

Embodiment 1

[0061] The Semen Cassiae control extract was prepared as follows:

[0062] (1) The AB-8 macroporous adsorption resin column of preparation example 1 is put on the sample solution of preparation example 1, the consumption ratio that makes cassia seed ethanol extract and AB-8 macroporous adsorption resin is 1g: 50ml, uses first successively The eluent and the second eluent are subjected to gradient elution to obtain the first eluent and the second eluent respectively, and the second eluent is collected and dried to obtain the semi-preparative component I; wherein, the The first eluent is 20vol% ethanol aqueous solution, and the consumption is 3 times of the volume of the macroporous adsorption resin column; the second eluent is 50vol% ethanol aqueous solution, and the consumption is 3 times of the volume of the macroporous adsorption resin column 5 times the amount;

[0063] (2) Dissolve the semi-preparative component I in a small amount of 50vol% ethanol aqueous solution, and ...

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Abstract

The invention discloses a cassia seed control extract, a preparing method and application thereof. The preparing method of a cassia seed control extract comprises: (1) packing a macroporous adsorptionresin column with the cassia seed alcohol extract to obtain a semi-prepared component I; (2) uniformly mixing the semi-prepared component I with a blank silica gel used in the sample mixing to obtaina sample mixed silica gel; (3) dry packing a chromatographic column with the blank silica gel, then packing the sample mixed silica gel and loading samples to obtain a silica gel chromatography column, and then eluting the silica gel chromatography column with an eluent; (4) evenly dividing the eluted silica gel chromatography column from the column head to the bottom into 18-24 parts, respectively adding methanol to elute to obtain methanol eluents, and detecting and combining the methanol eluates containing indicator components, then drying to obtain the cassia seed control extract. The preparing method of the cassia seed control extract is simple in process and suitable for large-scale preparation.

Description

technical field [0001] The invention relates to a cassia seed control extract and its preparation method and application, in particular to a cassia seed naphthopyrone reference extract, its preparation method and application. Background technique [0002] Quality control and evaluation of traditional Chinese medicine is an important scientific issue in the field of traditional Chinese medicine research. The current important quality control methods mainly use a single chemical component as a reference substance to control the quality of Chinese medicinal materials and compound prescriptions from qualitative and quantitative perspectives. This mode of using a single chemical component to represent the important overall quality cannot really control and evaluate the quality of traditional Chinese medicine, and it is also inconsistent with the holistic view of traditional Chinese medicine. The complexity of traditional Chinese medicine components determines that it is difficul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/14G01N30/34G01N30/02G01N30/90
CPCG01N30/02G01N30/06G01N30/14G01N30/34G01N30/90G01N2030/146
Inventor 刘斌姜艳艳周德勇王路骆宜
Owner BEIJING UNIV OF CHINESE MEDICINE
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