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A rice accase mutant gene and its application in plant herbicide resistance

A mutant, rice technology, applied in applications, plant products, genetic engineering, etc., to achieve the effect of significant resistance function

Active Publication Date: 2021-10-26
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the patent of the ACCase site mutation in rice that can resist the herbicide Gaygrass

Method used

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  • A rice accase mutant gene and its application in plant herbicide resistance
  • A rice accase mutant gene and its application in plant herbicide resistance
  • A rice accase mutant gene and its application in plant herbicide resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 Rice M3 Mutant Resistance to Acetyl-CoA Carboxylase Herbicides (Gai Cao Neng)

[0059] 150 kg of japonica conventional rice seed Jiahua No. 1 (purchased from Jiangsu Agricultural Germplasm Resources Protection and Utilization Platform) (this is M0, soaked in water for 2 hours) was divided into 6 times with 0.5-1.0% (w / w) formazan Soak in ethyl sulfonate (EMS) at room temperature for 6-9 hours, shake the seeds every 1 hour during this period; discard the EMS solution, soak the seeds 5 times in tap water, 5 minutes each time, then rinse the seeds with tap water overnight, and carry out the next day Sow seeds in the field, and carry out conventional fertilizer and water management (this is M1). After the plants are mature, the seeds are mixed, dried, and stored for the winter. Sow in the field the following year. When the rice (this is M2) seedlings grow to the 3-4 leaf stage, spray 1.5mL of Gai Caoneng / L water, and after 30 days, the normal green plants are ri...

Embodiment 2

[0060] Example 2: Mutation site analysis of rice mutant JH-7 resistant to acetyl-CoA carboxylase herbicides

[0061] The leaves of the obtained herbicide-resistant mutant rice plant JH-7 were selected and genomic DNA was extracted, and sent to Nanjing Yidao Biotechnology Co., Ltd. for genome sequencing. The sequencing results were compared with the wild-type ACCase gene of Jiahua No. 1, and it was found that there was a mutation at one site in the ACCase gene, and the 5191st base was mutated from C to T, resulting in a change in the corresponding encoded amino acid sequence. The 1731st position is changed from arginine to cysteine, that is, the nucleotide sequence of the ACCase gene of the herbicide-resistant mutant is shown in SEQ ID NO.1, and the amino acid sequence of the ACCase protein encoded by it is shown in SEQ ID NO .2 shown. The rice mutant is named as rice seed JH-7 (Oryza sativa Japonica Group JH-7), and the strain has been preserved in China Center for Type Cultu...

Embodiment 3

[0062] Example 3: Mutation site analysis of rice mutant JH-8 resistant to acetyl-CoA carboxylase herbicides

[0063]The herbicide-resistant mutant rice plant JH-8 selected multiple mutant leaves, extracted genomic DNA, and sent it to Nanjing Yidao Biotechnology Co., Ltd. for genome sequencing. The sequencing results were compared with the wild-type ACCase gene of Jiahua No. 1, and it was found that there was a mutation at one site in the ACCase gene, the 6826th base was mutated from T to G, resulting in a change in the corresponding encoded amino acid sequence. The 2276th position is changed from tyrosine to aspartic acid, that is, the nucleotide sequence of the ACCase gene of the herbicide-resistant mutant is shown in SEQ ID NO.3, and the amino acid sequence of the ACCase protein encoded by it is shown in SEQ ID NO .4 shown. The rice mutant is named rice seed JH-8 (Oryza sativa Japonica Group JH-8), and the strain has been preserved in the China Center for Type Culture Colle...

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Abstract

The invention discloses a rice ACCase mutant gene and its application in plant herbicide resistance. The amino acid sequence of the encoded protein of the ACCase mutant gene has the following mutation: it corresponds to the 1731st amino acid sequence of wild-type rice ACCase mutation at position 2276 or / and mutation at position 2276. The ACCase mutant gene is introduced into and expressed in the herbicide-sensitive rice genome by a transgenic method, and the recipient plant can have resistance (tolerance) to acetyl-CoA carboxylase inhibitor herbicides. The ACCase mutant gene can be widely used in plant herbicide resistance breeding.

Description

technical field [0001] The invention relates to a rice ACCase mutant gene and its application in plant herbicide resistance, and belongs to the field of plant genetic engineering and plant herbicide resistance breeding. The amino acid sequence of the protein encoded by the gene corresponds to the amino acid of wild type rice ACCase A mutation occurs at position 1731 or / and 2276 of the sequence. Background technique [0002] Acetyl-CoA carboxylase (ACCase) inhibitors are ultra-efficient herbicides, which have excellent control effects on grass weeds and are highly safe on dicotyledonous plants, so they are currently most commonly used in broad-leaved crop fields. But at the same time, it was found that long-term single use of this type of herbicide will generally increase the dosage year by year, and the efficacy will decrease, that is to say, the sensitive weeds will be greatly reduced, while the non-sensitive drug-resistant weeds will gradually increase and evolve into comm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/16C12N15/55C12N15/82A01H5/00A01H5/10A01H6/46
CPCC12N9/16C12N15/8274C12Y301/03044
Inventor 张保龙王金彦凌溪铁
Owner JIANGSU ACAD OF AGRI SCI
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