Fluorescent quantitative internal reference genes, primers and applications for different developmental stages of Changshan leaves in Haizhou

Abstract

The present invention discloses a fluorescent quantitative internal reference gene suitable for different development stages of Changshan leaves in Haizhou and its primers and applications. The internal reference genes are Actin, RAN and SAND genes, wherein the gene sequence of the Actin gene is shown in SEQ ID NO.1, The gene sequence of the RAN gene is shown in SEQ ID NO.6, and the gene sequence of the SAND gene is shown in SEQ ID NO.8. Based on the existing published genes, this application screens out candidate genes with relatively stable expression, evaluates their stability through software, obtains fluorescent quantitative internal reference genes suitable for different developmental stages of Changshan leaves in Haizhou, and uses these genes as a basis to design a real-time Fluorescent quantitative PCR primers fill in the current situation that there are no internal reference genes in different leaf stages of Haizhou Changshan in different provenances, and provide strong support for the accurate quantification of related functional genes in different leaf stages of Haizhou Changshan in different provenances, which can improve the research efficiency. Stability, repeatability and reliability.

Description

technical field [0001] The invention belongs to the field of plant molecular biology, and specifically relates to a fluorescent quantitative internal reference gene suitable for different development stages of Haizhou Changshan leaves, primers and applications thereof. [0002] technical background [0003] Haizhou Changshan (Clerodendrum trichotomum Thunb.), also known as stinky phoenix tree, is a deciduous shrub or small tree under the Verbenaceae (Verbenaceae) Daqing genus (Clerodendmm L.). It is resistant to barrenness, pollution, salt and alkali, and pests and diseases. It can be used for greening abandoned land, restoration and reconstruction of vegetation in arid barren hills and abandoned mining areas. It is an excellent tree species for saline-alkali land greening. The leaves of Haizhou Changshan are large, not only edible, but also used as medicinal materials, which can be used for rheumatic arthralgia, hemiplegia, hypertension, migraine, malaria, dysentery, hemorrh...

AI Technical Summary

Problems solved by technology

However, the research on the molecular mechanism of Haizhou Changshan has not been reported yet. According to the research, there is no internal reference gene that is constantly expressed under all experimental conditions. The commonly used internal reference genes will show unstable traits under different plants and experimental conditions, thus affecting The accuracy of target gene detection, so to develop good genes in Haizhou Changshan leaves, it is necessary to determine stable and reliable internal reference genes

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