Method and device for measuring fluorescence quantum yield
A fluorescence quantum yield and fluorescence photon technology, applied in the field of fluorescence quantum yield measurement, can solve the problems of inaccurate fluorescence quantum yield, inaccurate number of absorbed excitation light photons, etc.
Active Publication Date: 2021-05-18
ZOLIX INSTR
View PDF5 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
However, the current scheme for measuring the fluorescence quantum yield has the problem of inaccurate determination of the number of photons emitted by fluorescence and the number of photons absorbed by excitation light, resulting in inaccurate final measured fluorescence quantum yield
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0029] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be described in further detail below in conjunction with the embodiments and accompanying drawings. Here, the exemplary embodiments and descriptions of the present invention are used to explain the present invention, but not to limit the present invention.
[0030] Before introducing the technical solutions provided by the examples of the present invention, the professional names involved in the solutions provided by the examples of the present invention are firstly introduced.
[0031] 1. Fluorescence quantum yield: the ratio of the number of photons of fluorescence emitted by a fluorescent substance after absorbing light to the number of photons of absorbed excitation light.
[0032] 2. Sample to be tested: the sample to be tested can be solid sample, liquid sample, powder sample, gas sample and other samples that can have a stable state
[0033...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention provides a method and device for measuring fluorescence quantum yield, the method comprising: obtaining the number of first emitted fluorescent photons and remaining photons, the second emitted fluorescent photons and remaining photons, the third emitted fluorescent photons and remaining photons , the fourth emitted fluorescent photons and the number of remaining photons; according to the second emitted fluorescent photons and the remaining photons, the fourth emitted fluorescent photons and the remaining photons, determine the homogenized absorptivity; according to the homogenized absorptivity, the first emitted fluorescent photons and The number of remaining photons, the third emitted fluorescent photons and the remaining photons, determine the direct absorptivity; according to the first emitted fluorescent photons and the remaining photons, the direct absorptivity, determine the number of photons of excitation light absorbed by the sample to be measured; according to the third emitted fluorescent photons and the number of remaining photons, the fourth emitted fluorescence photon and the number of remaining photons, the homogenized absorption rate, the direct absorption rate, determine the number of fluorescent photons emitted by the sample; determine the fluorescence quantum yield. The above scheme improves the accuracy of measuring the fluorescence quantum yield.
Description
technical field [0001] The invention relates to the technical field of fluorescence quantum yield measurement, in particular to a method and device for measuring fluorescence quantum yield. Background technique [0002] Fluorescence quantum yield refers to the ratio of the number of photons of fluorescence emitted by a fluorescent substance after absorbing light to the number of photons of absorbed excitation light. Therefore, when testing fluorescence quantum, it is necessary to accurately measure the number of photons and absorption of emitted fluorescence. The number of photons of the excitation light. At present, the system for measuring fluorescence quantum yield includes excitation light source and its optical path, integrating sphere (built-in sample holder) and other devices that can homogenize light, signal collection optical path, disperser, detector and A / D converter, It is composed of software, computer and other units, and the responsivity of the system is cali...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/6402
Inventor 石广立张恒
Owner ZOLIX INSTR