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Method for determining content of hypoxanthine in Shuxuetong injection

A technology for hypoxanthine and injection, which is applied in the field of accurate determination of hypoxanthine content in Shuxuetong injection, can solve the problems of high requirements for chromatographic separation skills, heavy workload, complicated operation steps, etc., and achieve good specificity , reduce interference, high sensitivity effect

Inactive Publication Date: 2019-02-22
MUDANJIANG YOUBO PHARMA CO LTD +1
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  • Application Information

AI Technical Summary

Problems solved by technology

The above operations either require high chromatographic separation skills of the staff, or the operation steps are complicated and the workload is large

Method used

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  • Method for determining content of hypoxanthine in Shuxuetong injection
  • Method for determining content of hypoxanthine in Shuxuetong injection
  • Method for determining content of hypoxanthine in Shuxuetong injection

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Embodiment 1

[0039] Sample preparation

[0040] Preparation of the stock solution: accurately weigh the hypoxanthine, add an appropriate amount of methanol, sonicate it at 60°C for 30 minutes to completely dissolve it, let it cool, and dilute with methanol to prepare a 100μg / ml hypoxanthine methanol stock solution. In addition, accurately weigh 6-mercaptopurine, add an appropriate amount of methanol, vortex for 2 minutes to completely dissolve, and dilute with methanol to prepare a 250 μg / ml 6-mercaptopurine methanol stock solution.

[0041] Preparation of standard solution: accurately pipette 1.00ml hypoxanthine stock solution and dilute it with methanol-0.2% formic acid aqueous solution (2:8) into a 50μg / ml hypoxanthine standard solution for later use; in addition, accurately pipette 6-mercaptopurine stock Solution, diluted with methanol-0.2% formic acid aqueous solution (2:8) into 125μg / ml 6-mercaptopurine internal standard solution for later use.

[0042] Preparation of spiked samples: Prec...

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Abstract

The invention relates to a method for determining the content of hypoxanthine in Shuxuetong injection. The method includes adding a 6-purinethol internal standard solution and a hypoxanthine standardsolution into the Shuxuetong injection to be tested to prepare a labeled sample; adopting an LC / MS / MS method to conduct multi-reaction monitoring MRM scanning and conducting linear regression with thefinal concentration X of the labeled hypoxanthine as the abscissa and the peak area ratio Y of a target analyte to the internal standard as the ordinate to obtain a regression equation; adding the 6-purinethol internal standard solution to the Shuxuetong injection, adopting the LC / MS / MS method to conduct multi-reaction monitoring MRM scanning to obtain the peak area ratio of the target analyte tothe internal standard and obtain the content of the hypoxanthine in the Xuexuetong injection according to the above regression equation. According to the method, the content of the hypoxanthine in the Shuxuetong injection can be detected rapidly and accurately, and the interference of the matrix effect is effectively reduced.

Description

Technical field [0001] The invention belongs to the technical field of drug analysis, and relates to a quantitative analysis method, in particular to a method for accurately determining the content of hypoxanthine in Shuxuetong injection. Background technique [0002] Shuxuetong injection is composed of leeches and earth dragons. It has the effects of activating blood to remove blood stasis, clearing the meridians and activating collaterals. It is used in the acute stage of ischemic stroke caused by blood stasis and obstruction of collaterals. Skewed, slurred language. Hypoxanthine is one of the main intermediate products in the metabolism of purine bases in the organism. It has biological activity. It can enter the cell through the cell membrane, improve the activity of various enzymes, and participate in the regulation of some important physiological functions of the human body. The effect is particularly obvious, so hypoxanthine was selected as the content determination index...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72
Inventor 李振国王晓明潘桂湘王献瑞倪开岭郝明贾力夫赵西子胡利民刘二伟王丹丹
Owner MUDANJIANG YOUBO PHARMA CO LTD