Dichromatic curved reflector structure for improving signal-to-noise ratio of fluorescence detector

A technology of fluorescence detector and mirror, which is applied in the direction of fluorescence/phosphorescence, instruments, measuring devices, etc., can solve the problem of rarely reflecting excitation light, so as to prevent the increase of background noise and improve the detection signal-to-noise ratio

A technology of fluorescence detector and mirror, which is applied in the direction of fluorescence/phosphorescence, instruments, measuring devices, etc., can solve the problem of rarely reflecting excitation light, so as to prevent the increase of background noise and improve the detection signal-to-noise ratio

CN109387490AActive Publication Date: 2019-02-26DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Dichromatic curved reflector structure for improving signal-to-noise ratio of fluorescence detector
  • Dichromatic curved reflector structure for improving signal-to-noise ratio of fluorescence detector
  • Dichromatic curved reflector structure for improving signal-to-noise ratio of fluorescence detector

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Experimental program
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Effect test

Embodiment 1

[0029] The optical system is self-built, and the dichromatic curved mirror structure is set in the laser-induced fluorescence orthogonal detection system. System structure such as figure 2 As shown, it includes a dichroic curved mirror 1 (curved concave lens 3, dichroic film 4), light absorption box 2, laser 5, convex lens 6, capillary 7, optical fiber 8, optical filter 9, and detector 10. The curved surface of the dichroic curved mirror 1 is a spherical surface, the center of which is coincident with the axis of the capillary 7 and the excitation point of the laser, and its concave surface faces the direction of the detector 10 . During operation, after the excitation light is emitted from the laser 5, it is focused on the capillary 7 through the convex lens 6. The excitation point on the capillary radiates mixed light to the surroundings simultaneously, including sample fluorescence and laser stray light, wherein the mixed light that deviates from the detection direction s...

Embodiment 2

[0033] The optical system is self-built, and the dichromatic curved mirror structure is set in the laser-induced fluorescence orthogonal detection system. System structure such as image 3As shown, it includes a dichroic curved mirror 1 (curved concave lens 3, dichroic film 4), light absorption box 2, laser 5, convex lens 6, capillary 7, optical fiber 8, filter 9, detector 10, through hole 12 . The curved surface of the dichroic curved mirror 1 is an ellipsoid, and its near mirror focus coincides with the axis of the capillary 7 and the laser excitation point, its concave surface faces the direction of the detector 10, and its far mirror focus coincides with the optical fiber 8 port. During operation, after the laser light is emitted from the laser 5 , it is focused by the convex lens 6 and irradiated on the capillary 7 through the through hole 12 . The excitation point on the capillary radiates mixed light to the surroundings simultaneously, including sample fluorescence an...

Embodiment 3

[0037] Self-built optical system, the dichroic curved mirror structure is set in the laser-induced fluorescence orthogonal detection system, the structure is as follows Figure 4 As shown, it includes a dichroic curved mirror 1 (curved concave lens 3, dichroic film 4), light absorption box 2, laser 5, convex lens 6, capillary 7, filter 9, detector 10, through hole 12, lens 14 . Among them, the curved surface of the dichroic curved mirror 1 is a paraboloid, its focal point coincides with the axis of the capillary 7 and the laser excitation point, and its concave surface faces the direction of the detector 10 . When the system is working, the laser is emitted from the laser 5, focused by the convex lens 6, and irradiated on the capillary 7 through the through hole 12. The excitation point on the capillary radiates mixed light to the surroundings simultaneously, including sample fluorescence and laser stray light, wherein the mixed light that deviates from the detection directio...

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Abstract

The invention provides a dichromatic curved reflector structure for improving the signal-to-noise ratio of a fluorescence detector. The reflector provided by the invention is composed of a concave spherical lens with a concave surface coated with a dichromatic film, and a light-absorbing box closely attached to the back part of the concave spherical lens. The reflector only reflects fluorescence (with a reflectivity larger than 85%), rarely reflects excitation light (with a reflectivity less than 5%), while unreflected excitation light penetrates through the dichromatic spherical reflector toreach the light-absorbing box and is fully scattered and absorbed in the light-absorbing box, so the problem that fluorescent light and excitation stray light are reflected together is solved, and thesignal-to-noise ratio of a detector is further improved.

Description

technical field [0001] The patent of the present invention relates to a reflection mirror structure for a fluorescence detector, more specifically, to a dichromatic curved surface reflection mirror structure for improving the signal-to-noise ratio of a fluorescence detector. Background technique [0002] Fluorescence detector is a kind of ultra-high sensitive detector, which plays an important role in the detection of ultra-trace molecular level. The sensitivity of the detector is one of the important parameters to measure its performance, the greater the fluorescence collection efficiency of the detector, the greater the sensitivity. In principle, the fluorescence generated by the excitation region irradiated by the excitation light is spatially divergent, and the detector can only collect the fluorescence at a small part of the angle, so the collection efficiency of the fluorescence is very low. In order to improve the collection efficiency of fluorescence, people arrange...

Claims

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Application Information

Patent Timeline
26 Feb 2019
Publication
CN109387490A
IPC
G01N21/64
CPC
G01N21/6402
Inventors
耿旭辉; 邹郡