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A method for increasing protocorm polysaccharide content and its medium and application

A culture medium and protocorm technology, applied in the biological field, can solve the problems of low seed germination rate, unfavorable large-scale production, difficult pretreatment, etc., achieve easy sowing operation, improve seed germination rate and utilization rate, shorten in vitro culture propagation and the effect of breeding time

Active Publication Date: 2022-03-08
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Orchids have a large number of seeds, but the germination rate of mature and cracked fruits is low and it is difficult to pretreat, and the infection rate after sowing is high, which is not conducive to the needs of large-scale production

Method used

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  • A method for increasing protocorm polysaccharide content and its medium and application
  • A method for increasing protocorm polysaccharide content and its medium and application
  • A method for increasing protocorm polysaccharide content and its medium and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Embodiment 1, research on the embryonic development of Dendrobium candidum and the influence of different factors on the aseptic germination of Dendrobium candidum

[0084] (1) Embryo development of Dendrobium officinale

[0085] 1. Test materials

[0086] The test materials were collected in the greenhouse of the Institute of Flower and Botany, Guangxi Academy of Agricultural Sciences. On May 22, 2014, robust Dendrobium officinale plants were selected for artificial pollination, and the fruits were collected at intervals of 5 days for the test.

[0087] 2. Test method

[0088] 1. Stereo microscope morphology observation

[0089] After collecting the fruit, cross-cut the fruit with a double-sided blade, observe the morphological characteristics and development status of the fruit and seeds 0-180 days after pollination under a stereomicroscope (Leica DFC500), and select periods with typical morphological characteristics to take pictures. Morphological characteristics ...

Embodiment 2

[0178] Embodiment 2, dendrobium officinale suspension culture production polysaccharide

[0179] 1. Protocorm material for solid culture

[0180] 1. The test materials were collected in the Flower Plant Research Institute of Guangxi Academy of Agricultural Sciences. On May 22, 2014, robust Dendrobium candidum plants were selected for artificial pollination, and the fruits of 120DAP, 150DAP and 180DAP were collected, and 3 fruits were collected each time for the test.

[0181] 2. Seed pretreatment

[0182] Uncracked fruits were removed from the surface, and the surface of the fruit was cleaned with a toothbrush. The fruit was placed in a beaker and rinsed with running water for 1 hour, and then transferred to an aseptic operating table for sterilization. The sterilization procedure is: 75% alcohol for 30 seconds; soaking in 1% sodium hypochlorite solution for 15 minutes; washing with sterile water for 5 times, each time for 2 minutes. The sterilized fruit is taken out, blotte...

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Abstract

The invention discloses a method for increasing the content of protocorm polysaccharides, a culture medium used therefor, and applications. Through the research on embryo development of Dendrobium officinale, combined with the morphological characteristics of seeds of different ages, the invention optimizes the fast propagation system, determines the seed age suitable for aseptic germination and sowing, and sows the suitable seed age Dendrobium officinale seeds in the improved culture medium, Protocorms with high germination rate and uniform germination state were obtained; the protocorms were inoculated into different liquid media for suspension culture to produce polysaccharides, and the optimal medium for suspension culture of Dendrobium officinale was finally determined. The method of the invention greatly shortens the in vitro propagation and breeding time of Dendrobium officinale, the improved liquid medium significantly increases the polysaccharide content in the protocorm of Dendrobium officinale, and selects Dendrobium officinale seeds of suitable age as explants for tissue culture and fast propagation The large-scale and industrialized production cycle is greatly shortened, and the theoretical basis and reference value are provided for the production of polysaccharides using protocorm instead of whole grass.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for increasing the content of protocorm polysaccharides, a culture medium and application thereof. Background technique [0002] Dendrobium belongs to Orchidaceae, and most of the plants in the genus have important medicinal and ornamental value. Dendrobium contains about 1000 species of plants, mainly distributed in tropical and subtropical regions of Asia. In my country, Dendrobium plants are concentrated in the provinces and regions south of the Qinling Mountains, especially in the southern part of Yunnan Province (Chen Xinqi, 1999), of which there are about 50 species. species can be used medicinally (Baiyin, 2007). The 2010 edition of the Pharmacopoeia of the People's Republic of China includes five kinds of medicinal Dendrobium: Dendrobium loddigesii, Dendrobium fimbriatum, Dendrobium chrysanthum, Dendrobium nobile and Dendrobium officinale. Among them, th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 程瑾刘岩辛小雪沐先运刘忠华马文柯
Owner BEIJING FORESTRY UNIVERSITY
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