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Isolated culture and rapid propagation method of cut-flower ornithogalum caudatum

A technology of cut flowers with ten thousand years of tiger eyes and cut flowers with ten thousand eyes of tiger eyes, applied in the field of agriculture, can solve the problems of low bulb reproduction coefficient, prolonged seedling growth period, small and weak bulb scales, etc., and achieve the effect of ensuring the survival rate of seedlings and increasing yield

Inactive Publication Date: 2019-03-08
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing breeding method of Evergreen is mainly scale cutting breeding, but due to the low propagation coefficient of its bulbs, the scale cutting operation is cumbersome, laborious and laborious, and the production efficiency is low (the bulb belongs to a skinned bulb, and the scales are difficult to strip, and the bulbs have small scales. Weak, low seedling rate of cutting propagation), unable to meet the growing market demand
However, the existing tissue culture rapid propagation systems all use a single technical means to achieve rapid seedling growth (such as: leaf induction, pedicel induction or even flower bud induction, etc.), failing to apply the tissue culture rapid propagation methods to the best state, It leads to prolonging the period from the establishment of the sterile line to the establishment of seedlings, the low yield of seedlings produced in each batch, and the high production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Selection and processing of explants:

[0031] On January 7, 2017, 10 healthy bulbs (bulbs free from diseases and insect pests) of the cut flower Evergreen Princestar variety were placed at a low temperature of 4°C for 14-16 days until the bulbs grew 0.5-1 cm to germinate.

[0032] On January 23, 2017, the explants were collected and sterilized. The specific operation method: remove the aged and shriveled bulb skin of the above-mentioned bulbs to expose the hypertrophic part, and use a scalpel to divide the bulb into four petals from top to bottom along the longitudinal direction of the bulb. , and do not scratch the heart bud, the bottom of each petal has a corresponding bulb base, peel off a single complete heart bud, and peel each petal into a single scale, and each scale and the bottom of the heart bud have a corresponding bulb At the base, rinse the scales and heart buds 2-3 times with water, with HgCl at a concentration of 0.1% w / w 2 Soak in the solution for ...

Embodiment 2

[0045] Embodiment 2 is the same as Embodiment 1 except that the following steps are different.

[0046] (1) Selection and processing of explants:

[0047] On January 10, 2018, 15 healthy bulbs (bulbs without pests and diseases) of the cut flower Evergreen Yellowstar variety were placed at a low temperature of 4°C for 14-16 days until the bulbs grew 0.5-1 cm and germinated. In 2018 The explants were collected and sterilized on January 26.

[0048] (2) Establishment of sterile line and start-up culture:

[0049] The starting medium is: MS+6-BA 2mg / L+NAA 0.1mg / L+sucrose 30g / L+agar 6.25g / L, pH 6.0; 25°C±2°C, light time 12h / d, culture for 14-16d until the heart bud grows into a rootless plant, and the base of the rootless plant differentiates into young buds.

[0050] (3) Proliferation culture:

[0051] Culture conditions: light intensity 1500-2000lx, temperature 25°C±2°C, light time 10h / d, proliferation medium: 1 / 2MS+6-BA 1mg / L+NAA 0.1mg / L+sucrose 30g / L+agar 6.25g / L, pH 6.

...

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PUM

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Abstract

Disclosed is an isolated culture and rapid propagation method of cut-flower ornithogalum caudatum. The isolated culture and rapid propagation method has the advantages that two types of explants obtained by one-time bulb pretreatment are inoculated into a starting culture medium through sterilization, then subjected to multiplication culture, rooting culture and seedling transplant, and subjectedto different culture modes and conditions according to the types during culture, so that the cardinal number of materials is increased remarkably, the seedling yield is increased greatly, and the production efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a method for in vitro culture and rapid propagation of cut flowers Dieffenbachia tiger's eye. Background technique [0002] Tiger eye evergreen (Ornithogalum caudatum Jacq.), also known as squill, ornithogalum caudatum Jacq., is a perennial herb of the family Liliaceae and the genus Ornithogalum caudatum Jacq. At present, a batch of tiger eye evergreen cut flower varieties have emerged in the international flower market. The flower branches are longer, the flowers are dense, the cut flowers have good water absorption, and the ornamental period of vases is as long as more than one month. Arranging flowers with its main material, together with carnation, calla lily, variable-leaf wood, and carnation, is elegant and indifferent. It is very elegant and durable to place on the window sill and in the living room. [0003] With the continuous provision of living standar...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 阮继伟屈云慧杨春梅田林孟汪国鲜吴丽芳单芹丽余蓉培李帆吴旻
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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