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A kind of preparation method of pinanoside Pb of dianthus parvum

A technology of pinanoside and parenchyma, which is applied in the field of rapid extraction and preparation of pinanoside Pb by macroporous resin enrichment and column chromatography, which can solve the problems of inability to scale production, high toxicity of organic solvents, and environmental problems. pollution and other issues

Active Publication Date: 2021-07-06
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The purpose is to provide a method for extracting and separating pinanoside Pb from Zhonglou diancaoside by using ethanol extraction as raw material, so as to solve the toxicity of organic solvents used in the separation and preparation of pinanoside Pb from the medicinal material of Zhonglou diancao Large, serious environmental pollution, technical problems that cannot be scaled up

Method used

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  • A kind of preparation method of pinanoside Pb of dianthus parvum
  • A kind of preparation method of pinanoside Pb of dianthus parvum
  • A kind of preparation method of pinanoside Pb of dianthus parvum

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Embodiment 1

[0029] The method for extracting and isolating pinanoside Pb from Chonglou diancao, specifically comprises the following steps:

[0030] (1) Grind the dry Chinese Rhizoma Dianchi into coarse powder (1.0kg), add 1 to 2 times (about 2L) of the weight of the medicinal material of Dian Zhong, and ethanol with a volume percentage concentration of 80%, and reflux extraction for 2-2 3 times, 1-3 hours each time, the extraction temperature is 80-85°C, the extract is filtered, the extracts are combined, and then vacuum-concentrated into a solution with an alcohol content of 5% to 10%, and filtered to obtain the filtrate.

[0031] (2) Put the filtrate obtained above on a macroporous adsorption resin column (3kg, the inner diameter of the column is 8.5cm), first use a weight of 15 to 20 times (about 20L) of the weight of the medicinal material of Zhonglou, wash it with pure water and discard the eluent ; then use the weight to be 10 to 20 times of the weight of the medicinal material of ...

Embodiment 2

[0036] The method for extracting and isolating the saponin Pb of Paris polyphylla from Pachyphyllum yunnanensis specifically comprises the following steps:

[0037] (1) Grind the dried Zhonglou medicinal material into coarse powder (10kg), add 4-6 times the weight of the medicinal material (about 50L) and ethanol with a concentration of 80% by volume, and reflux for 2-3 Once, 1-3 hours each time, the extraction temperature is 80-85°C, the extract is filtered, the extracts are combined, and then vacuum-concentrated into a solution with an alcohol content of 5% to 10%, and filtered to obtain the filtrate.

[0038] (2) Put the filtrate obtained above on a macroporous adsorption resin column (30kg, the inner diameter of the column is 27cm), first use a weight of 15 to 20 times (about 200L) of the weight of the medicinal material of Dianzhonglou, wash it with pure water and discard the eluent ; then use the weight to be 10 to 20 times of the weight of the medicinal material of Dian...

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Abstract

The invention discloses a rapid and high-efficiency preparation method of picoside Pb. The method comprises three steps of ethanol extraction, macroporous resin enrichment of total saponins, and column chromatography separation to obtain pinanoside Pb from dianthus syringae, and the purity of the obtained sarcoside Pb is more than 95%. The method adopts ethanol as the extractant, and the extraction is sufficient; the macroporous resin enriches the total saponins completely; and the single pinanoside Pb is effectively obtained through the silica gel column and the YMC column. The extraction solvent adopted in the method has low toxicity, simple and feasible process, low cost, simple operation steps and easy scale-up, and is very suitable for large-scale production.

Description

technical field [0001] The invention belongs to the technical field of extracting plant active ingredients, and in particular relates to a method for extracting and separating pinanoside Pb from the medicinal material of Papaya chinensis, in particular to a method for rapidly extracting and preparing Pinazoside Pb by macroporous resin enrichment and column chromatography. F. Pinanoside Pb method. Background technique [0002] Chonglou is a perennial herbaceous plant of the genus Paris L. in the family Triliaceae. There are 27 species in the world, and 25 species in my country, of which 19 species are distributed in the southwest of China. Most of them use their rhizomes as medicine. The traditional Chinese medicine commonly used in our country has been recorded in the "Chinese Pharmacopoeia" over the years. The 2015 edition of the "Chinese Pharmacopoeia" includes the species of Parispolyphylla Smith Var.chinensis (Franch.) Hara and Paris polyphylla Smithvar.yunnanensis (Franc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J71/00
CPCC07J71/0005
Inventor 杨立新李良群和云和兵
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI