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Application of D-serine as marker in preparing depression diagnostic kit as well as depression diagnostic kit

A diagnostic kit and technology for depression, applied in disease diagnosis, biological testing, biological material analysis, etc., can solve the problems of lack of biomarkers, lack of objective basis and standards, incompletely clear etiology of depression, etc., and achieve improvement. Depression-like behavior, effects of increasing neuronal excitability

Inactive Publication Date: 2019-03-08
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, the etiology of depression is still not completely clear, and most of the current clinical diagnosis of depression relies on the scoring methods of various scales, such as the commonly used anxiety self-rating scale (Self-rating Anxiety Scale). -ratinganxiety scale, SAS) or self-rating depression scale (Self-rating depression scale, SDS)
Regardless of the form of the scale, it belongs to the category of subjective diagnosis and lacks objective basis and standards.
At present, there is still a lack of objective indicators such as biomarkers related to the diagnosis of depression

Method used

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  • Application of D-serine as marker in preparing depression diagnostic kit as well as depression diagnostic kit
  • Application of D-serine as marker in preparing depression diagnostic kit as well as depression diagnostic kit
  • Application of D-serine as marker in preparing depression diagnostic kit as well as depression diagnostic kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] The establishment of embodiment 1 mouse depression model

[0065] The onset of depression is different from other genetic psychiatric diseases, and often occurs after adulthood. Therefore, this example establishes a rodent animal model of depression that can be induced in adulthood.

[0066] Construction of transgenic animals: mutant DISC1 protein is fused with HA-labeled estrogen receptor ligand, and administration of tamoxifen drug can induce the expression of this protein and induce depression-like behavior when mice are adults. The result is as Figure 1A As shown, it can be seen from the figure that HA is expressed in the cerebral cortex, hippocampus and nucleus accumbens, indicating that the transgenic animal was successfully constructed.

[0067] The establishment of the rodent depression animal model established in this embodiment is reflected by the reduction of social barriers and forced swimming struggle time:

[0068] Social Disorders: The Three-Box Social ...

Embodiment 2

[0076] Example 2 Detection of D-serine level

[0077] In this embodiment, the level of D-serine is detected by the following method:

[0078] 1. In vivo microdialysis to obtain mouse samples;

[0079] 2. Purify the sample through the column;

[0080] 3. Make the standard curve of D-serine with the depression diagnosis kit provided by the invention;

[0081] 4. In the same way, replace the standard with cerebrospinal fluid samples;

[0082] 5. Incubate for 60 minutes at 37°C;

[0083] 6. Use a microplate reader to detect Ex / Em=539 / 587nm, endpoint mode.

[0084] 7. Calculate according to the formula obtained from the standard curve.

[0085] Specific neural circuits in the depressed brain function abnormally, and this abnormality is often caused by abnormal function of some nerve cells. Studies have shown that in the depression model, the function of glial cells is abnormal, and the mice have depression-like behaviors. The in vivo microdialysis test results of the depress...

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Abstract

The invention provides application of D-serine as a marker in preparing a depression diagnostic kit as well as the depression diagnostic kit and relates to the technical field of depression diagnosis.The depression diagnostic kit is characterized in that metabolic substances with abnormal lesion in brain tissues of depression patients are researched; research results shows that the D-serine is closely related with depression, so that the D-serine as the marker is put forward for the first time to be applied to a relevant field of preparing the depression diagnostic kit, the defect of unobjective diagnosis of indexes used for the depression diagnosis process at present are overcome, so that brand-new higher-accuracy objective diagnosis standards are provided for the later depression diagnosing, and a new idea is further provided for diagnosing the depression. The depression diagnostic kit provided by the invention can quickly and accurately detect the concentration of the D-serine in cerebrospinal fluid of subjects, and thereby effectively and accurately judge whether the subjects are suffered from the depression or not as well as the severity of the depression.

Description

technical field [0001] The invention relates to the technical field of depression diagnosis, in particular to the application of D-serine as a marker in the preparation of a depression diagnosis kit and the depression diagnosis kit. Background technique [0002] People's depression is a negative emotion, and if it lasts for too long, it will form pathological mental diseases, such as depression. Depression is a psychiatric disease caused by multiple reasons, with persistent low mood as the main symptom. Its clinical manifestations are diverse, often accompanied by anxiety, anhedonia, and social impairment. Depression is a common psychiatric disease, and the World Health Organization predicts that by 2020, depression will become the second most common disease. [0003] The pathogenesis of depression is complex, involving multi-system and multi-link dysfunction, which can be manifested as abnormal dynamic balance of various biochemical substances. Unfortunately, the etiology...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/304
Inventor 屠洁周辛夷肖倩谢丽鲁艺杨帆
Owner SHENZHEN INST OF ADVANCED TECH
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