Method for increasing vinblastine content in vinca rosea cells
The technology of vinblastine and vinblastine is applied in the field of increasing the content of vinblastine in vinca cells, can solve the problems of long cultivation period and high cultivation cost of plant cells, and achieve the effects of shortening the induction period, avoiding browning and increasing the biosynthesis amount.
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Embodiment 1
[0021] A method for improving vinblastine content in vinca cells, comprising the following steps:
[0022] 1) Select plump periwinkle seeds, soak them in weak acid electrolyzed water for 10 seconds, wash them with sterile deionized water, and use them on the B5 foundation containing 10mg / L vitamin C, 7g / L agar and 0.1g / L oligosaccharin The aseptic seedlings were cultured on the culture medium, and the hypocotyls of the aseptic seedlings were used as explants, which were inserted into the callus induction medium for induction culture. The culture temperature was 32°C, and the light and dark cultures were alternately carried out every day. Induce the callus, then transfer the callus to the subculture medium, subculture once every 25 days, and subculture 3 times to obtain stable periwinkle callus;
[0023] 2) Select the periwinkle callus with vigorous growth, loose texture, and stable and uniform state, inoculate it into a liquid suspension medium, then add glass beads to the liq...
Embodiment 2
[0027] A method for improving vinblastine content in vinca cells, comprising the following steps:
[0028] 1) Select plump periwinkle seeds, soak them in weak acid electrolyzed water for 10 seconds, wash them with sterile deionized water, and use them on the B5 foundation containing 10mg / L vitamin C, 7g / L agar and 0.1g / L oligosaccharin The aseptic seedlings were cultured on the culture medium, and the hypocotyls of the aseptic seedlings were used as explants, which were inserted into the callus induction medium for induction culture. The culture temperature was 32°C, and the light and dark cultures were alternately carried out every day. Induce the callus, then transfer the callus to the subculture medium, subculture once every 25 days, and subculture 3 times to obtain stable periwinkle callus; wherein, the callus induction Medium is the B5 basal medium containing 1mg / L cytokinin, 0.5mg / L 6-benzylaminopurine, 30g / L sucrose, 3mg / L licorice flavone and 8g / L agar;
[0029] 2) Se...
Embodiment 3
[0033] A method for improving vinblastine content in vinca cells, comprising the following steps:
[0034] 1) Select plump periwinkle seeds, soak them in weak acid electrolyzed water for 10 seconds, wash them with sterile deionized water, and use them on the B5 foundation containing 10mg / L vitamin C, 7g / L agar and 0.1g / L oligosaccharin The aseptic seedlings were cultured on the culture medium, and the hypocotyls of the aseptic seedlings were used as explants, which were inserted into the callus induction medium for induction culture. The culture temperature was 32°C, and the light and dark cultures were alternately carried out every day. Induce the callus, then transfer the callus to the subculture medium, subculture once every 25 days, and subculture 3 times to obtain stable periwinkle callus; wherein, the callus induction Medium is the B5 basal medium containing 1mg / L cytokinin, 0.5mg / L 6-benzylaminopurine, 30g / L sucrose, 3mg / L licorice flavonoids and 8g / L agar; Add phenyla...
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