Method for establishing model for pseudomonas aeruginosa based empyema drainage tube biological film of rabbit

A technology for Pseudomonas aeruginosa and model establishment, which is used in drug delivery, pharmaceutical formulations, medical raw materials derived from bacteria, etc.

Active Publication Date: 2019-03-15
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] (1) At present, there is no mature animal model of empyema drainage tube biofilm formation;
[0009] (2) At present, there is no mature animal model of biofilm formation in empyema drainage tubes infected by Pseudomonas aeruginosa

Method used

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  • Method for establishing model for pseudomonas aeruginosa based empyema drainage tube biological film of rabbit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1. Take the Pseudomonas aeruginosa strain to resuscitate, enrich the bacteria, and dissolve it in LB broth to make a bacterial solution of the required concentration.

[0045] 2. Anesthesia: place the rabbit in a rabbit operation box, and inject 2% sodium pentobarbital (30 mg / kg) into the ear vein to anesthetize the rabbit.

[0046] 3. Fixation: Move the rabbit to the operating table, take the left lying position, bind and fix it, and fully expose the right anterior chest wall area.

[0047] 4. Shave and prepare the skin on the 4th, 5th, 6th, and 7th ribs of the right front chest.

[0048] 5. Positioning: Touch the ribs on the right chest wall, start from the 12th rib, touch the 6th intercostal space, select the middle and outer part of the sternum-spine as the puncture point, mark with a pen, and repeat the positioning twice.

[0049] 6. Disinfection: Disinfect the skin around the puncture point with povidone iodine, with a diameter of about 10cm, twice, and spread a ...

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Abstract

The invention belongs to the technical field of animal models and discloses a method for establishing a model for a pseudomonas aeruginosa based empyema drainage tube biological film of rabbit. The method comprises steps as follows: preparation of a bacterial liquid; anesthetization: the rabbit is anesthetized by intravenous injection of 2% pentobarbital sodium on the ear margin; fixing, shaving and skin preparation; positioning; disinfecting; puncture: the skin at the sixth intercostal puncture point on the right side is cut open to form an incision about 1.0 cm, straight tweezers puncture the pleura, a scalp needle tube is clamped, arranged in and fixed, an injection syringe is connected, and withdrawing is performed until no gas can be extracted; injection of the bacterial liquid: the bacterial liquid with concentration being 2 ml / kg in terms of weight of the rabbit is injected; after the scalp needle tube is tightly clamped, the head end is cut off, the residual tube is rapidly sent into the chest by the tweezers, and the skin wound is sutured; the chest of the rabbit is dissected after the surgery, empyema is formed, a large amount of pus exists in the chest, and the drainagetube is found in the chest. With adoption of a crystal violet staining method, formation of the biological film in the drainage tube is proved, and the model for the pseudomonas aeruginosa based empyema drainage tube biological film of rabbit is established.

Description

technical field [0001] The invention belongs to the technical field of animal models, and in particular relates to a method for establishing a biofilm model of a Pseudomonas aeruginosa rabbit empyema drainage tube. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] Pseudomonas aeruginosa is one of the common Gram-negative bacilli in empyema infection, accounting for 12% to 30% of all Gram-negative bacilli. [0004] The results of the drug susceptibility test indicated that the Pseudomonas aeruginosa derived from the pus was multi-drug resistant, which led to the poor treatment effect of empyema, prolonged healing, and the formation of chronic empyema. Biofilms are aggregates of organized growth of microorganisms. Microorganisms growing in the form of biofilms are more resistant to antibacterial drugs and host immune defenses than planktonic bacteria. Thoracentesis and indwelling drainage tube to drain ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74
CPCA61K9/0019A61K35/74
Inventor 王可张真强孔晋亮李金垄韦球
Owner GUANGXI MEDICAL UNIVERSITY
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