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A kind of rpe cell sheet and its application and preparation method

A cell sheet and cell technology, applied in the medical field, can solve the problems of limited donors and restricted applications of natural materials, and achieve the effects of low immune rejection, good biocompatibility, and good biocompatibility

Active Publication Date: 2021-12-10
AIER EYE HOSPITAL GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limited supply of natural materials, its application is limited.

Method used

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  • A kind of rpe cell sheet and its application and preparation method
  • A kind of rpe cell sheet and its application and preparation method
  • A kind of rpe cell sheet and its application and preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0063] The preparation method of the present embodiment RPE cell sheet is as follows:

[0064] 1. After femtosecond surgery, corneal lens material itself and decellularized as scaffold material are as follows:

[0065] 1.1 After the full femtosecond laser operation, the thin layer of corneal tissue in the shape of a convex lens cut from the cornea was taken out and stored in normal saline or glycerin.

[0066] 1.2 Rinse with PBS 3 times, put in 1.5M NaCl solution, change it every 24 hours, shake for 48 hours in total.

[0067] 1.3 Shake with 5U / ml DNAse and 50μg / mL RNAse for 48 hours.

[0068] 1.4 Wash with PBS for 72 hours and change the solution every 24 hours.

[0069] 2. Utilize the RPE cell of iPS conditioned medium culture or the RPE cell of iPS source to construct the tissue engineering slice method as follows:

[0070] 2.1 Collect the supernatant of 50%-80% confluent iPS cells, and add it to the ordinary medium of DMEM / F12+10% FBS at a ratio of 1 / 2 to culture RPE ce...

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Abstract

The invention relates to the field of medical technology, in particular to an RPE cell sheet and its application and preparation method. The RPE cell sheet includes a corneal lens and an RPE cell layer. The RPE cell sheet of the present invention has good biocompatibility and low immune rejection, and the cell sheet is superior to cell sheets constructed in other ways in terms of barrier, phagocytosis and other functional detection. Vision problems of patients with retinal degenerative diseases such as macular degeneration; and corneal lens is abundant, so it is a good and cheap substitute for Bruch's membrane.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to an RPE cell sheet and its application and preparation method. Background technique [0002] Retinal diseases such as age-related macular degeneration (AMD), retinitis pigmentosa (RP, night blindness), diabetes, arterial or venous occlusion, and even blindness are due to the degeneration of the retina and the death of photoreceptor cells. With the continuous improvement of my country's aging population, the incidence of AMD is also increasing. Studies have shown that the prevalence of early AMD in China is 5.7%. Compared with India (4.5%) and Malaysia (3.55%), which are also located in Asia, my country has the highest prevalence of early AMD. Therefore, AMD has brought a certain degree of threat to the development of our country's society and economy. There are two main clinical types of AMD, one is dry (atrophic) and the other is wet (neovascular). Dry AMD is mainly caused by...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/38A61L27/50C12N5/079
CPCA61L27/3604A61L27/3813A61L27/3834A61L27/50C12N5/0621A61L2430/16
Inventor 顾佳宁王译妮陈建苏唐仕波
Owner AIER EYE HOSPITAL GRP CO LTD
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