Protein chip and kit for early diagnosis of lung cancer

A protein chip and kit technology, applied in disease diagnosis, instrument, biomaterial analysis, etc., can solve the problems of inconvenient detection, slow speed, large amount of serum samples, etc., to improve sensitivity and specificity, less sample demand, The effect of strong sensitivity

Active Publication Date: 2022-02-11
GUANGZHOU BIO BLUE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Early diagnosis and treatment of lung cancer are the key to improving the survival rate of patients. However, most lung cancers detected by current diagnostic methods are in the middle and advanced stages, and the best treatment period has been missed.
In addition, the traditional ELISA detection of serum antibodies is slow and inefficient, and requires a large amount of serum samples, which is very inconvenient for specific detection

Method used

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  • Protein chip and kit for early diagnosis of lung cancer
  • Protein chip and kit for early diagnosis of lung cancer
  • Protein chip and kit for early diagnosis of lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Embodiment 1 Preparation of a kit containing a protein chip for early diagnosis of lung cancer (liquid protein chip diagnostic kit)

[0092] Preparation of antigenic protein chip (liquid chip): purchase commercially available magnetic microbeads from Luminex, firstly couple biotin-BSA to magnetic microbeads-COOH through an amino coupling reagent, and use the purification and recombination of the 10 antigens prepared above Proteins (containing Streptavidin labels), through the combination of biotin on the surface of magnetic microbeads and the Streptavidin labels contained in antigens, respectively bind 10 antigens to the surface of magnetic microbeads of different colors. Then add a layer of PBS containing 20% ​​fetal bovine serum on the surface of the magnetic microbeads, place it at room temperature for 1 hour, and then place it overnight at 4°C to fully seal the non-specific protein cross-linking or binding sites on the magnetic microbeads, and finally divide Pack an...

Embodiment 2

[0094] Example 2 Using the kit of Example 1 to diagnose and screen suspected patients with lung cancer and healthy people

[0095] Using the kit described in Example 2, 200 cases of lung cancer patients, 200 cases of normal controls, 2000 cases of high-risk groups of lung cancer, and 1000 cases of general community population were detected by protein chip plus immunofluorescence method. 200 cases of normal controls were used to define the diagnostic criteria of lung cancer to judge the results. The results are shown in Table 1.

[0096] Table 1 uses the kit of embodiment 1 to diagnose and screen lung cancer suspected patients and healthy people

[0097]

[0098] Among 200 cases of lung cancer patients and 200 cases of normal controls, the kit detected 158 positive cases of lung cancer patients and 20 cases of normal people. Therefore, the sensitivity of the kit for diagnosing lung cancer was 79.0%, and the specificity was 90.0%, indicating that The kit has high sensitivit...

Embodiment 3

[0102] Example 3 uses other detection kits in combination to improve the specificity and sensitivity of early diagnosis of lung cancer.

[0103] Application No. 201610007369.4, titled Protein Chip, Protein Chip Diagnostic Kit Preparation and Application Method The protein chip and kit in the patent application detect the relative content of autoantibodies (IgG and IgM) corresponding to six lung cancer-related autoantigens, To determine whether the subject has lung cancer. The six antigens are (1) P53 antigen fragment; (2) SOX2 antigen fragment; (3) COPB1 antigen fragment; (4) EFHD2 antigen fragment; (5) EIF4G3 antigen fragment; (6) PCNA antigen fragment. The sensitivity of the kit for diagnosing lung cancer is relatively high, especially for the early stage (I stage), the sensitivity is 82.0%, and the specificity is 88.0%.

[0104] Combined use of the kit described in Example 1 and the above 6 kinds of lung cancer antigen autoantibody detection kits, using protein chip plus i...

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Abstract

The invention relates to a protein chip and a kit for early diagnosis of lung cancer. The protein chip includes the following ten antigen fragments: MAGE A3 antigen fragment, ANXA1 antigen fragment, CTAG1B antigen fragment, CAGE1 antigen fragment, AP3D1 antigen fragment, CCT8 antigen fragment, CHK antigen fragment, PRKACA antigen fragment, EIF4G1 antigen fragment and MAGE A1 antigen fragment. The protein chip and kit for early diagnosis of lung cancer creatively select antigens that are significantly expressed in the early stage of lung cancer and can cause the immune system to produce related antibodies, and screen the corresponding antigen fragments for combination. Using protein chip technology, the antigen fragments Coated on the surface of a solid support to detect tumor-associated autoantibodies in human serum to achieve early diagnosis of lung cancer, with a high detection positive rate, which can greatly improve the sensitivity and specificity of early lung cancer diagnosis.

Description

technical field [0001] The invention relates to the field of cancer diagnosis and treatment, in particular to a protein chip and a kit for early diagnosis of lung cancer. Background technique [0002] Lung cancer is one of the malignant tumors with the fastest increasing morbidity and mortality and the greatest threat to the health and life of the population. In the past 50 years, many countries have reported that the morbidity and mortality of lung cancer have increased significantly. At present, the diagnosis of lung cancer mainly relies on imaging and cytology, but early lung cancer has no obvious signs, and the positive rate of current detection methods is very low, often only 10-15% of patients can be detected and diagnosed. Early diagnosis and treatment of lung cancer are the key to improving the survival rate of patients. However, most lung cancers detected by current diagnostic methods are in the middle and advanced stages, and the best treatment period has been mis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574
CPCG01N33/57423G01N33/57488G01N2800/60
Inventor 林当胡海黄静赵晓龙周海鹰许勇黄璐潘敬东
Owner GUANGZHOU BIO BLUE TECH CO LTD
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