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Method for assisting determination of hematological stage of childhood acute lymphoblastic leukemia

A leukemia and hematology technology, applied in biochemical equipment and methods, microbiological measurement/testing, computer-aided medical procedures, etc., can solve the problems that the usefulness has not yet been concluded, MRD monitoring markers are insufficient, etc.

Pending Publication Date: 2019-03-15
OTSUKA PHARM CO LTD
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Problems solved by technology

However, no conclusions have been drawn regarding the usefulness of WT1 mRNA quantification as a prognostic predictor of childhood leukemia and monitoring of MRD
In addition, according to Non-Patent Document 11, it is pointed out that the above-mentioned WT1 mRNA is sufficient as a marker for monitoring MRD in adult AML patients, but it is insufficient as a marker for monitoring MRD in pediatric ALL patients.
The reason for this has not been clarified, but it is believed that adult ALL patients and children ALL patients have various gene abnormalities with different frequencies, or the prognosis of the two is also different (Non-Patent Documents 12-13). ALL for different diseases

Method used

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  • Method for assisting determination of hematological stage of childhood acute lymphoblastic leukemia
  • Method for assisting determination of hematological stage of childhood acute lymphoblastic leukemia
  • Method for assisting determination of hematological stage of childhood acute lymphoblastic leukemia

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example

[0277] Hereinafter, examples are given to describe the present invention in more detail. However, the present invention is not particularly limited to this example.

example 1

[0279] Using "WT1 mRNA Measurement Kit II "Otsuka"" (manufactured by Otsuka Pharmaceutical Co., Ltd.), which is a Wilms tumor-1 gene (WT1) mRNA kit, for children with ALL and children suspected of having ALL Subjects, the amount of WT1 mRNA in RNA extracted from peripheral leukocytes and bone marrow fluid nucleated cells was measured. The kit is composed of mixed solution for RT-PCR, metal ion solution (manganese acetate, etc.), WT1 and GAPDH mixed solution RNA standard solution (WT1RNA, GAPDH RNA, etc.), and standard solution diluent (buffer, etc.), etc. . In addition, the RT-PCR mixture contained the WT1 forward primer, WT1 reverse primer, WT1 probe, GAPDH forward primer, GAPDH reverse primer, and GAPDH probe shown in Table 5. The 5' end of the WT1 probe was labeled with HEX (6-hexachlorofluorescein), and the 5' end of the GAPDH probe was labeled with FAM (6-carboxyfluorescein). In addition, the 3' end of each probe was labeled with ATTO-540Q (ATTO-TEC GmbH) as a quencher ...

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Abstract

Provided are a method for assisting the determination of hematological stage of childhood acute lymphoblastic leukemia (childhood ALL) and an extracorporeal diagnostic agent usable in the method. Themethod for assisting the determination of hematological stage of childhood ALL comprises: (1) a step for acquiring the mRNA quantity of Wilms' tumor gene 1 (WT1) in biological sample(s) comprising theperipheral blood and / or the bone marrow fluid of a subject; (2) a step for acquiring the mRNA quantity of GAPDH in the above sample(s); and (3) a step for calculating an index value necessary for assisting the determination on the basis of the ratio between the WT1 mRNA quantity and the GAPDH mRNA quantity that are acquired respectively in the above steps (1) and (2).

Description

technical field [0001] The invention relates to a method for assisting the judging of the hematology stage of children's acute lymphoblastic leukemia. Specifically, it relates to a method for discriminating whether a child subject suffering from or suspected of having acute lymphoblastic leukemia is in a non-remission phase or a remission phase of acute lymphoblastic leukemia. In addition, the present invention relates to an in vitro diagnostic drug that is effective for assisting in the judgment of the hematological stage of acute lymphocytic leukemia, in particular, assisting in the judgment of transition from the non-remission phase to the remission phase, and from Auxiliary judgment for transition from remission to non-remission, or monitoring of hematological stages of acute lymphoblastic leukemia. Background technique [0002] The kit (WT1 mRNA measurement kit) for measuring the amount of WT1 mRNA (Wilms' tumor gene 1 messenger ribonucleic acid, Wilms' tumor-1 gene me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12N15/09C12Y102/01009C12Q1/6886C12Q2600/112C12Q2600/158C12Q1/68G16H50/20C12Q1/686C12Q2600/106C12Q2600/118C12Q2600/156
Inventor 宫崎良秀梅原縁小坂修首藤公義
Owner OTSUKA PHARM CO LTD
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