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Application of bacteroides fragilis extract to preparation of medicines or foods for prevention and treatment of irritable bowel syndrome

A technology for irritable bowel syndrome and Bacteroides fragilis, applied in the field of medicine or food, can solve the problems that IBS cannot be completely cured, and there is a lack of treatment methods for IBS.

Active Publication Date: 2019-03-19
GUANGZHOU ZHIYI PHARMA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] At present, there is still no very effective treatment for IBS, and IBS cannot be completely cured

Method used

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  • Application of bacteroides fragilis extract to preparation of medicines or foods for prevention and treatment of irritable bowel syndrome
  • Application of bacteroides fragilis extract to preparation of medicines or foods for prevention and treatment of irritable bowel syndrome
  • Application of bacteroides fragilis extract to preparation of medicines or foods for prevention and treatment of irritable bowel syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] The preparation of embodiment 1 Bacteroides fragilis extract

[0074] (1) Fermentation culture of Bacteroides fragilis

[0075] Streak inoculation of strains on blood plate, anaerobic culture for 48h. Observe the colony morphological characteristics, staining characteristics, size, club shape and distribution, etc.

[0076] Colony characteristics: Bacteroides fragilis ZY-312, after being cultured on a blood plate for 48 hours, is slightly convex, translucent, white, smooth, non-hemolytic, and the diameter of the colony is between 1-3mm. See figure 1 .

[0077] Morphology under the microscope: Bacteroides fragilis ZY-312 was examined by Gram staining. It is a Gram-negative bacterium with a typical rod shape, blunt rounded ends and dense staining. The uncolored part in the middle of the bacteria is like a vacuole. figure 2 .

[0078] A single colony was selected and inoculated in tryptone broth for fermentation and culture for 8 hours (at a temperature of 37° C.). Th...

Embodiment 2

[0087] Example 2 Effect of Bacteroides fragilis capsular polysaccharide A on Senna-induced diarrhea-type IBS

[0088] 1. Experimental design

[0089] In order to verify the effect of the Bacteroides fragilis extract (the main component is capsular polysaccharide A) provided in Example 1 on the prevention / treatment of irritable bowel syndrome, 60 C57BL / 6 mice were selected for the experiment in this example. 60 C57BL / 6 mice were divided into male and female, and each experimental mouse was assigned a unique number. Prior to grouping animals, the item number, species / strain, sex, cage number, and animal number should be marked on the cage label. Use BioBook software to carry out random grouping according to the initial body weight of C57BL / 6 mouse, be divided into 6 groups, namely normal group (Group1), model group (Group2), loperamide capsule group (2.4mg / kg) (Group3), Bacteroides fragilis capsular polysaccharide A low (Group4), medium (Group5), high (Group6) dose groups, 10 ...

Embodiment 3

[0099] Example 3 Effect of Bacteroides fragilis capsular polysaccharide A on castor oil-induced diarrhea-type IBS

[0100] 1. Experimental design

[0101] In order to verify the effect of the Bacteroides fragilis extract (the main component is capsular polysaccharide A) provided in Example 1 on the prevention / treatment of irritable bowel syndrome, 60 C57BL / 6 mice were selected for the experiment in this example. 60 C57BL / 6 mice were divided into male and female, and each experimental mouse was assigned a unique number. Prior to grouping animals, the item number, species / strain, sex, cage number, and animal number should be marked on the cage label. Use BioBook software to carry out random grouping according to the initial body weight of C57BL / 6 mouse, be divided into 6 groups, namely normal group (Group1), model group (Group2), loperamide capsule group (2.4mg / kg) (Group3), Bacteroides fragilis capsular polysaccharide A low (Group4), medium (Group5), high (Group6) dose groups...

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Abstract

The invention relates to application of bacteroides fragilis extract to preparation of medicines or foods for prevention and treatment of an irritable bowel syndrome. The bacteroides fragilis extractcontains bacteroides fragilis capsular polysaccharide A. Accidentally, the bacteroides fragilis capsular polysaccharide A with the molecular weight of 5-70KD is prepared, a great irritable bowel syndrome prevention and treatment function of the bacteroides fragilis capsular polysaccharide A with the molecular weight of 5-70KD is discovered, and the bacteroides fragilis capsular polysaccharide A with the molecular weight of 5-70KD is much more effective than capsular polysaccharide A, with the molecular weight of 110KD, extracted from bacteroides fragilis NCTC 9343.

Description

technical field [0001] The invention relates to the technical field of application of Bacteroides fragilis, in particular to the application of an extract of Bacteroides fragilis in preparing medicine or food for preventing and treating irritable bowel syndrome. Background technique [0002] Irritable bowel syndrome (IBS) is the most common intestinal dysfunction in clinical practice. In recent years, it has been recognized as a kind of psychosomatic disease with special pathological and physiological basis. , constipation, diarrhea, or alternating constipation and diarrhea, lack of morphological or biochemical abnormalities syndrome. The global incidence of IBS is very high, and the incidence rate is between 10% and 15%. In Western countries, IBS accounts for 12% of family health physician visits and 20% to 50% of gastroenterology visits. The rate of IBS symptoms in my country is the same as that in foreign countries. The patients are mainly young and middle-aged people, ...

Claims

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Application Information

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IPC IPC(8): A61K35/741A61P1/12A61P1/10A61P1/00A23L33/135A23L33/125A23K10/16A23K20/163A23C9/13A23C19/09A23G9/36
CPCA61K35/741A61P1/00A61P1/12A23C9/13A23C19/09A23G9/36A23K10/16A23K20/163A23L33/125A23L33/135A61K31/715A61K35/74A61P1/10C12N1/20Y02A50/30
Inventor 刘洋洋智发朝王晔白杨王从峰
Owner GUANGZHOU ZHIYI PHARMA INC
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