Fingerprint spectrum construction method and detection method of Yangjing Zhongyu prescription

A technology for raising essence and seeding jade and fingerprinting, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of decoction, granule, decoction determination, etc., and achieve the effect of good reproducibility and high precision

Active Publication Date: 2019-03-22
GUANGZHOU XIANGXUE PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regarding the detection of the preparations of Yangjing Yufang, there is no unified method and standard for the comprehensive determination of decoctions, granules, and decoctions.

Method used

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  • Fingerprint spectrum construction method and detection method of Yangjing Zhongyu prescription
  • Fingerprint spectrum construction method and detection method of Yangjing Zhongyu prescription
  • Fingerprint spectrum construction method and detection method of Yangjing Zhongyu prescription

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] 1. Instruments and reagents

[0061] 1.1 Instrument: electronic balance Mettler Toledo XP205DR (d=0.01mg), Mettler ToledoMS204S (d=0.1mg), high-frequency numerical control ultrasonic cleaner (KQ-300TD, Kunshan Ultrasonic Instrument Co., Ltd.) Simplicity UV pure water machine (Millipore) , High-performance liquid chromatography (Agilent 1260), freeze dryer (Christ, ALPHA2-4LD plus, Germany), rotary evaporator N-1200A (EYELA Shanghai Instrument Co., Ltd.).

[0062] 1.2 Reagents: Acetonitrile and phosphoric acid are chromatographically pure, water is purified water, and other reagents are analytically pure.

[0063] 1.3 Test drug: reference substance of paeoniflorin (China Institute for Food and Drug Control, 110736-201539, 110736-201741), reference substance of ferulic acid (China Institute for Food and Drug Control, 110773-201313), reference substance of loganin (China Food and Drug Control Institute, 111640-201005, 111640-201707), morroniside reference substance (China...

Embodiment 2

[0164] In this example, the fingerprints prepared in Example 1 are used to detect unqualified Yangjing Zhongyu decoction with missing raw materials.

[0165] With reference to the method of 2.1 of Example 1, unqualified sample 1 (raw material formula is rehmannia glutinosa: wine angelica: wine cornus=2:1:1), unqualified sample 2 (raw material formula is rehmannia glutinosa: wine white peony root: Wine Cornus = 2:1:1), unqualified sample 3 (the raw material formula is Rehmannia glutinosa: wine angelica: wine white peony = 2:1:1), unqualified sample 4 (the raw material formula is wine angelica: wine white peony : wine cornus=1:1:1), and the method for preparing unqualified sample with reference to the method for 2.3 of embodiment 1 needs testing solution, the method for referring to embodiment 1 for 2.4 prepares mixed reference substance solution, then with reference to the method for embodiment 1 The chromatographic conditions of 2.2 and the assay method of 2.5 are used for det...

Embodiment 3

[0169] In this example, the fingerprints prepared in Example 1 are used to detect Yangjingzhongyujianpao.

[0170] The preparation of need testing solution: get about 1g of Yangjingzhongyu decoction paste, accurately weighed, add 50mL of 70% methyl alcohol precisely, close up, weigh, ultrasonic treatment (power 300W, frequency 40kHz) 30 minutes, take out, Allow to cool, weigh again, make up the lost weight with 70% (volume percent) methanol, shake well, filter, and take the filtrate to obtain the test solution. All the other operating methods are the same as in Example 1.

[0171] The results of the fingerprints showed that compared with the control fingerprints, all 14 common peaks appeared, and the similarity was above 0.95. The results show that the fingerprints constructed in Example 1 can also be used for quality control of Yangjingzhongyujianpao.

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Abstract

The invention discloses a fingerprint spectrum construction method and a detection method of a Yangjing Zhongyu prescription. The construction method comprises the following steps: preparing a test solution; preparing a reference solution; and constructing a fingerprint spectrum: sucking the test solution and the reference solution, and injecting the sucked test solution and the sucked reference solution into a high performance liquid chromatograph to be measured so as to obtain the fingerprint spectrum of the Yangjing Zhongyu prescription with a common characteristic peak. On the basis of long-term experience accumulation and a large number of experiments, the inventors select ferulic acid, paeoniflorin, morroniside and loganin as reference substances to construct the fingerprint spectrum, so that the quality of the classical Yangjing Zhongyu decoction can be well characterized, and the quality of the modern Yangjing Zhongyu condensed decoction and Yangjing Zhongyu granules can also be characterized. Especially, the fingerprint spectrum is constructed by selecting appropriate chromatographic conditions, so that the Yangjing Zhongyu decoction can be qualitatively and quantitativelycharacterized, and the Yangjing Zhongyu condensed decoction and the Yangjing Zhongyu granules can also be qualitatively and quantitatively characterized.

Description

technical field [0001] The invention relates to the field of quality control of traditional Chinese medicine preparations, in particular to a method for constructing a fingerprint and a method for detecting a formula of Yangjingzhongyu prescription. Background technique [0002] Yangjing Zhongyu Decoction is a traditional Chinese medicine prescription, which comes from the volume of "Fu Qingzhu Gynecology". Cornus officinalis 15 grams (steamed), mainly used for kidney deficiency and blood deficiency, emaciated body, long-term infertility. [0003] At present, the preparations of Yangjingzhongyu prescription on the market include Yangjingzhongyu decoction paste and Yangjingzhongyu granules. There have been literatures on the fingerprint and content determination of Yangjingzhongyu decoction. Jade granules have been studied on the content determination, but the research is only to establish the fingerprint comparison method of multiple batches of Yangjing Yujian extract, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 姚娜陈桂生康志英李雪银汪静黄燕明曾锦丽
Owner GUANGZHOU XIANGXUE PHARMA CO LTD
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