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Accurate resistance identification method of black rot of sweet potato at seedling stage

A technology for resistance identification and black spot disease, applied in the field of agricultural plant protection, can solve the problems of lack of identification of sweet potato black spot resistance, and achieve the method of avoiding spore fluid infecting potato seedlings, the method is scientific and fast, and the identification results are accurate and reliable. Effect

Pending Publication Date: 2019-03-22
江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心)
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Problems solved by technology

Therefore, the application of these two methods for the identification of sweet potato black spot resistance is lacking.

Method used

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  • Accurate resistance identification method of black rot of sweet potato at seedling stage
  • Accurate resistance identification method of black rot of sweet potato at seedling stage

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Embodiment 1

[0033] like figure 1 Shown, a kind of seedling stage sweet potato black spot precise resistance identification method specifically comprises the following steps:

[0034] 1) Isolation and activation of the pathogen: Collect the diseased potato pieces from the field, isolate and purify the sweet potato black spot fungus by the tissue isolation method and the single spore isolation method, and after the verification of the Koch's law, use the potato dextrose agar medium to cultivate for 7 days. spare;

[0035] 2) Preparation of PS culture medium: Weigh 20g of potatoes and add 1L of ultrapure water to boil until the potatoes are completely rotten and soft. Autoclave at 121°C for 15 minutes for later use;

[0036] 3) Shaking culture of pathogenic bacteria: Take out the activated sweet potato black spot bacteria, use a hole puncher with a diameter of 6mm to punch out the bacteria discs, inoculate 20 bacteria discs in each triangular flask, and place the inoculated triangular flas...

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Abstract

The invention discloses an accurate resistance identification method of black rot of a sweet potato at a seedling stage, and belongs to the field of agricultural plant protection. The accurate resistance identification method comprises the following steps of 1), separation and activation of a germ; 2), preparation of a PS (Polystyrene) culture solution; 3), shaking culture of a pathogen; 4), preparation of a black rot germ spore suspension solution; 5), preparation and inoculation of a to-be-tested sweet potato seedling; and 6), investigation and evaluation. The identification method providedby the invention is scientific and efficient, is simple to operate, adopts spore solution root-irrigation inoculation identification, and approaches the practical situation of a field sweet potato seedling infected with the black rot; an identification result is more accurate and reliable; meanwhile, the phenomena that a stalk of the sweet potato seedling easily decays in the disease-resistant identification process of a hydroponic sweet potato seedling and that a sweet potato piece is contaminated by sundry germs and easily decays after moisture preservation in the process of carrying out disease-resistant identification by adopting the sweet potato piece are avoided; the accurate resistance identification method is provided for the black rot of the sweet potato at the seedling stage; thepowerful guarantee is provided for quickly and massively screening a black rot resistant variety of the sweet potato at an early stage; and the accurate resistance identification method has importantapplication value for guiding the disease-resistant breeding of the sweet potato.

Description

technical field [0001] The invention relates to a method for accurately identifying resistance to sweet potato black spot at the seedling stage, belonging to the field of agricultural plant protection. Background technique [0002] Sweetpotato blackrot (sweetpotato blackrot) is one of the main diseases in sweetpotato cultivation and storage. It occurs in all sweetpotato producing areas in the world. organization, resulting in dead seedlings, rotten beds, and rotten cellars, which have a great impact on sweet potato production. The diseased potatoes infected by black spot bacteria contain substances such as sweet potato kabamycin, which can cause poisoning symptoms after being eaten by livestock; using diseased potatoes as fermentation raw materials will delay the fermentation process, reduce alcohol production and quality, and seriously restrict the development of the sweet potato industry. develop. Black spot fungus can be transmitted through various ways such as seed pot...

Claims

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Application Information

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IPC IPC(8): G01N33/00
CPCG01N33/0098
Inventor 杨冬静谢逸萍孙厚俊张成玲马居奎王波
Owner 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心)
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