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Method for measuring area of Hydrilla verticillata leaves by modified image processing method

A technology of black algae and image correction, applied in measuring devices, instruments, optical devices, etc., can solve the problem that it is difficult to measure the leaf area of ​​black algae

Inactive Publication Date: 2019-03-26
NANJING INST OF GEOGRAPHY & LIMNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The leaves of Hydrilla verticillium are about 1.5 cm long and 0.5-1 cm wide, with small serrations on the leaf margin and sessile leaves. Therefore, it is difficult to measure the leaf area of ​​Hydra vermicelli by conventional methods

Method used

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  • Method for measuring area of Hydrilla verticillata leaves by modified image processing method
  • Method for measuring area of Hydrilla verticillata leaves by modified image processing method
  • Method for measuring area of Hydrilla verticillata leaves by modified image processing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] This example involves determining the coefficients K and M in equation (2) described herein.

[0037] S1: Take 10 different leaves of Hydrilla verticillum and number them from 1# to 10# in sequence; then place the numbered leaves of Hydrilla verticillium in the center of the opaque plate with black border, illuminate the opaque plate with a light source, control the light intensity to 1800 lux, and use pixels A 1200dpi digital camera shoots down the leaves from a position 50cm directly above the center of the opaque plate, and obtains its image. Use computer interpretation to obtain the deformed leaf area of ​​H. verticillium with different numbers, record the leaf area of ​​H. verticillium measured by the image processing method from 1# to 10#, and record them as A1 to A10 in turn. See Table 1 for specific results. The schematic diagram of the method for measuring the leaf area of ​​this implementation can be found in figure 1 .

[0038] S2: Use the grid method to me...

Embodiment 2

[0043]This example involves determining the coefficients K and M in equation (2) described herein.

[0044] S1: Take 10 different leaves of Hydrilla verticillum and number them from 1# to 10# in sequence; then place the numbered leaves of Hydrilla verticillium in the center of the opaque plate with black border, illuminate the opaque plate with a light source, control the light intensity to 1800 lux, and use pixels A 1400dpi digital camera shoots down the leaves from a position 70cm directly above the center of the opaque plate, and obtains its image. Use computer interpretation to obtain the deformed leaf area of ​​H. verticillium with different numbers, record the leaf area of ​​H. verticillium measured by the image processing method from 1# to 10#, and record them as A1 to A10 in turn. See Table 2 for specific results.

[0045] S2: Use the grid method to measure the leaves of Hydrilla verticillium 1# to 10#, record the leaf area of ​​No. 1# to 10#, and obtain the leaf area ...

Embodiment 3

[0050] This example involves determining the coefficients K and M in equation (2) described herein.

[0051] S1: Take 10 different leaves of Hydrilla verticillum and number them sequentially from 1# to 10#; then place the numbered leaves of Hydrilla verticillium in the center of the opaque plate with black border, illuminate the opaque plate with a light source, control the light intensity to 1200 lux, and use pixels A 2400dpi digital camera shoots down the leaves from a position 50cm directly above the center of the opaque plate, and obtains its image. Use computer interpretation to obtain the deformed leaf area of ​​H. verticillium with different numbers, record the leaf area of ​​H. verticillium measured by the image processing method from 1# to 10#, and record them as A1 to A10 in turn. See Table 3 for specific results.

[0052] S2: Use the grid method to measure the leaves of Hydrilla verticillium 1# to 10#, record the leaf area of ​​No. 1# to 10#, and obtain the leaf are...

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Abstract

The invention relates to a method for measuring the area of Hydrilla verticillata leaves by a modified image processing method. The measuring method comprises the steps of placing the Hydrilla verticillata leaves on an opaque plate with a dark border of a known area in a non-overlapping manner, vertically downward photographing the opaque plate before and after the placement of the Hydrilla verticillata leaves and the Hydrilla verticillata leaves at a fixed height by using a camera of a specific pixel under a specific light intensity condition to obtain images of the opaque plate and an image of the leaves, reading the number of pixels of the dark border opaque plate and the number of pixels in a leaf portion by image processing software, and determining the area of the Hydrilla verticillata leaves based on an area pixel ratio of the opaque plate and the leaves. According to the method of the invention, the area of the Hydrilla verticillata leaves can be quickly and accurately obtained, and the method is particularly suitable for the measurement of the area of a large number of Hydrilla verticillata leaves.

Description

technical field [0001] The present application relates to the technical field of measuring leaf area, in particular, to a method for measuring the leaf area of ​​Hydrilla verticillium by correcting image processing. Background technique [0002] Submerged plants are the main primary producers and key food chain links in aquatic ecosystems, and play an important role in regulating the energy cycle, structure and function stability of aquatic ecosystems. In water bodies with eutrophication or serious water pollution, submerged plants can reduce nitrogen and phosphorus nutrients in eutrophic water bodies, increase dissolved oxygen, improve transparency, promote the settlement of suspended particles, and inhibit sediment resuspension and algae growth. Water quality improvement function. [0003] Plant leaves are the main organs of plants for photosynthesis, transpiration, and organic matter synthesis. Plant leaf area is a key parameter for estimating the intensity of plant phot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01B11/28
CPCG01B11/28
Inventor 邓建才张杰朱金格胡维平
Owner NANJING INST OF GEOGRAPHY & LIMNOLOGY