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Kit and method for simultaneously detecting content of multiple components in plasma and application of kit

A kit and content technology, applied in the biological field, can solve the problems of cumbersome inconvenience, low investigation sensitivity, poor accuracy, etc., and achieve the effects of saving consumption, solving the difficulty of sample collection, and high sensitivity

Inactive Publication Date: 2019-03-26
南京新耀医疗技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The main purpose of the present invention is to solve the problems of low sensitivity, poor accuracy and cumbersome inconvenience in the investigation of diseases related to acute abdominal pain in the prior art, and provide a kit for simultaneously detecting the contents of multiple components in plasma

Method used

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  • Kit and method for simultaneously detecting content of multiple components in plasma and application of kit
  • Kit and method for simultaneously detecting content of multiple components in plasma and application of kit
  • Kit and method for simultaneously detecting content of multiple components in plasma and application of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037]The kit for simultaneous determination of serum TPSII, hs-CRP, SSA, PCT and resistin provided in this example includes the following components: reagent one (R1), which contains phosphate buffer and polyethylene glycol; Reagent two (R2), which contains phosphate buffer, sensitized latex coupled with anti-TPSII monoclonal antibody a (hereinafter referred to as monoclonal antibody a), and anti-hs-CRP monoclonal antibody b (hereinafter referred to as The sensitized latex of monoclonal antibody b) for short, the sensitized latex stabilizer linked with anti-SSA monoclonal antibody c (hereinafter referred to as monoclonal antibody c), and the sensitized latex stabilizer linked with anti-PCT monoclonal antibody d (hereinafter referred to as monoclonal antibody d) ), sensitized latex with anti-resistin monoclonal antibody e (hereinafter referred to as monoclonal antibody e); TPSⅡ, hs-CRP, SSA, PCT and resistin positive quality control serum; TPSⅡ, hs- CRP, SSA, PCT and resistin ...

Embodiment 2

[0057] Accuracy of serum TPSⅡ, hs-CRP, SSA, PCT and resistin double latex enhanced immunoturbidimetric assay kit

[0058] (1) Accuracy and precision

[0059] Choose high, medium and low concentrations of standard products, each containing TPSⅡ, hs-CRP, SSA, PCT and resistin at a concentration of 500ng / ml, 200ng / ml, 450ng / ml, 450ng / ml and 300ng / ml, 200ng / ml, 150ng / ml, 180ng / ml, 170ng / ml and 140ng / ml, 50ng / ml, 30ng / ml, 100ng / ml, 70ng / ml and 75ng / ml, the latex enhanced immune turbidimetric method designed by the present invention The detection kit detects each concentration of the above samples 10 times, and compares the obtained results to determine the accuracy and precision of the kit. The obtained detection results are shown in Table 3:

[0060] Table 3 Comparison of test results of TPSⅡ, hs-CRP, SSA, PCT and resistin

[0061]

[0062]

[0063]

[0064] The T-test of paired samples was performed on the data of this group, and P>0.05 was obtained, and the differenc...

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Abstract

The invention discloses a kit and method for simultaneously detecting the content of multiple components in plasma and application of the kit, and belongs to the technical field of biology. The kit comprises a detecting solution R1, a detecting solution R2, antibiont marker positive quality control serum, antibiont marker negative quality control serum and a wash concentrate. The content of trypsinogen TPSII, SSA, hs-CRP, PCT and resistin in the human plasma is detected jointly based on latex enhanced turbidimetric immunoassay; and acute abdominal pain diseases caused by acute pancreatitis, other abdominal viscera inflamatory lesions, acute gastrointestinal ulcers and the like can be troubleshot, and the purpose of identifying the common acute abdominal pain diseases is achieved through multi-index joint detecting.

Description

technical field [0001] The invention relates to a biological kit, a method and an application thereof, in particular to a kit, a method and an application for simultaneously detecting the contents of multiple components in blood plasma, and belongs to the field of biotechnology. Background technique [0002] Acute abdominal pain (acute abdominal pain) is one of the most common symptoms of emergency patients, and its visits can occur throughout the year, with peaks in autumn (September-November) and summer (June-August), and the age of visits is concentrated between 15 and 44 years old (accounting for 72.7% of the total number of visits), often involving internal, external, pediatric, obstetrics and gynecology, and even neurological and mental diseases. Caused by systemic disease. According to statistics, the incidence of acute pancreatitis is the highest among non-traumatic acute abdominal pain diseases, followed by acute gastroenteritis, acute intestinal obstruction, acute...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/543G01N33/573G01N33/68
CPCG01N33/54313G01N33/54326G01N33/573G01N33/577G01N33/6893G01N2333/4733G01N2333/4737G01N2333/976G01N2800/062G01N2800/065G01N2800/067
Inventor 滕文臣王其亮杨子学管中来
Owner 南京新耀医疗技术有限公司
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