57 results about "Immunoturbidimetric Assays" patented technology

The invention relates to a kit for determining heart-type fatty acid binding protein in serum or urine by latex enhanced turbidimetric immunoassay. Specifically, the kit for determining the heart-type fatty acid binding protein comprises a reagent R1, a reagent R2 and a calibrator, wherein the reagent R1 contains a reaction promoter, an antiseptic, a surfactant, a stabilizing agent, an electrolyte and a buffer; the reagent R2 contains latex particles with binding of anti-heart-type fatty acid binding protein monoclonal antibody and polyclonal antibody, an antiseptic, a surfactant, a stabilizing agent, an electrolyte and a buffer; and the calibrator contains an antiseptic, an electrolyte, a stabilizing agent, a heart-type fatty acid binding protein pure product and a buffer. By the complex coating method of latex particles with the monoclonal antibody and the polyclonal antibody, high sensitivity and wide linear range of the kit are guaranteed. Simultaneously, the kit also has advantages of high accuracy, good repeatability, strong singularity, easy operation and the like, and is applicable to an automatic biochemical analyzer which is commonly used in clinic.

The invention relates to a kit of latex-enhanced immunoturbidimetry for detecting content of glycocholic acid in blood serum. Specifically, the provided glycocholic acid detection kit comprises a reagent R1, a reagent R2 and a calibrator, wherein the reagent R1 comprises a reaction promotion agent, a preservative, a surface active agent, a stabilizer, an electrolyte and a buffer solution; the reagent R2 comprises latex particles combined with a glycocholic acid antibody, a preservative, a surface active agent, a stabilizer, an electrolyte and a buffer solution; and the calibrator comprises a preservative, an electrolyte, a stabilizer, glycocholic acid pure products and a buffer solution. The kit for detecting the content of the glycocholic acid in the blood serum, disclosed by the invention, ensures the high sensitivity and wide linear range of a kit by utilizing a method of coating the latex particles by utilizing polyclonal antibodies, also has the advantages of high accuracy, good repeatability, strong specificity, simplicity in operation and the like, and can be applied to a clinical general full automatic biochemical analyzer.

The invention provides a gastric cancer detecting method, a reagent and a gastric cancer detecting kit. The kit comprises a reagent R1 and a reagent R2 or R3; in vitro, the serum of a tested body is mixed with the reagent R1, and the reagent R2 or R3 is added after several minutes; latex particles included in the reagent R2 or R3 are coated with PG antibodies and can be combined with the PG antigen in a sample to form an insoluble complex; based on a latex-enhanced immunoturbidimetry principle, the absorbancy tested by a biochemical analyzer is compared with that of a calibration product, so that the content level of PG is calculated; compared with the content level of the serum PG of healthy people, the content level of PG shows that a health condition of the stomach of the tested human body is initially judged. By comparing a test result obtained by using the kit with that obtained by using an imported kit A, the kit has the advantages that the extremely-high correlation is achieved, and the correlation coefficient is more than or equal to 0.9750. The kit provided by the invention can be used for detecting the gastric cancer, and has stable and reliable detection results; moreover, the use cost can be reduced.

The invention provides a CRP latex-reinforced immunonephelometry reagent. The CRP latex-reinforced immunonephelometry reagent comprises CRP antibody-labeled latex particles, a buffer, a surfactant, an inorganic salt, a stabilizing agent, a suspending assistant, an excipient and an antiseptic. The invention also provides a kit for single-reagent CRP latex-reinforced immunonephelometry and a use thereof. The CRP latex-reinforced immunonephelometry reagent has simple composition, can be used simply and conveniently, has a fast reaction rate, high sensitivity and good repeatability, can be operated by a simple apparatus, has a wide apparatus adaptation range, has a low cost, can be widely used in large, middle and small hospitals and is suitable for clinical fast infection diagnosis and infection prognosis evaluation.

The invention relates to a kit for determining myeloperoxidase (MPO) content in serum. The invention aims to solve the technical problem to overcome the defects in the background art and provides a kit for determining myeloperoxidase content by enhanced turbidimetric immunoassay, which has the advantages of no need of dilution of a sample, simplicity in operation, high accuracy, good repeatability and suitability and is applied to various full-automatic biochemical analyzers and various special protein instruments. The invention adopts a technical scheme that the kit for determining the myeloperoxidase content by enhanced turbidimetric immunoassay comprises the following components: a, a reagent R1 which comprises buffer solution, a preservative, an accelerating agent, inorganic salt, a surface active agenet and the balance of purified water; b, a reagent R2 which comprises buffer agent, antibody combined with anti-human myeloperoxidase, a preservative, wherein the diameter of latex microspheres is 60-150nm; c, a reference calibration material which comprises buffer solution, a stabilizing agent, a preservative, a recombinant human myeloperoxidase pure product in a certain amount determined by concentration requirement and the balance of purified water. By the reagent combination, the calibration curve of MPO content is established, thereby achieving rapid determination of the MPO content in the serum on the full-automatic biochemical analyzer or the special protein instrument.

The invention discloses a neutrophil gelatinase associated lipocalin (NGAL) chemiluminescence detection kit. The kit comprises the components of a standard substance of NGAL, an NGAL monoclonal antibody labeled by horseradish peroxidase, magnetic particles coated by the NGAL monoclonal antibody, a white non-transparent microwell plate, washing liquid, and chemiluminescence substrates A and B. The kit provided by the invention can be used for detecting the content of the NGAL in blood serum of a patient, and has important guiding significance to the detections on premature kidney diseases and injures. Compared with the conventional ELISA (Enzyme Linked Immunosorbent Assay) method, the NGAL chemiluminescence detection kit provided by the invention has the characteristics of being high in sensitivity and good in specificity and overcoming the large influence of immunoturbidimetry by blood fat concentration, and has the advantages of high stability, reliability and accuracy, security and environment friendliness, simplicity and convenience in operation and the like.

The invention provides a method for simply and quickly detecting hoptoglobin in serum, namely an immunoturbidimetry, and a detecting kit thereof. The method is a detecting method designed by using the principle that the hoptoglobin is a human protein, has antigenicity and generates cohesion after combining with an antigen antibody. The hoptoglobin in the serum sample is combined with adequate specific antibody in the agent to form an insoluble immune complex; the change of the absorbance of the reaction solution is relevant to the content of the hoptoglobin in the serum sample. The method for detecting the hoptoglobin in the serum and the detecting kit in the invention have the advantages that: the serum sample is not subjected with special treatment, and can be ordinarily detected on an automatic biochemistry analyzer; the result directly reflects the true protein content of the hoptoglobin in the serum sample; therefore, the invention is used for clinic detection.

The invention discloses a beta 2-microglobulin detection kit which comprises a reagent R1 and a reagent R2, and the reagent R1 is a buffer solution; the reagent R2 is a mixture of beta 2-microglobulin antibody sensitization polystyrene latex particles and a buffer solution. The detection kit has the advantages that the detection is simple and rapid, the sensitivity is high, the accuracy is good, the disturbance resistance is high and the production cost is low. An adopted beta 2-microglobulin detection method is latex enhanced immunoturbidimetry, the beta 2-microglobulin detection is more economical, convenient and rapid with the method, and the beta 2-microglobulin detection kit is suitable for automatic biochemistry analyzers in vast majority of hospitals, and particularly suitable for realizing rapid quantitative detection on emergency treatment.

The invention discloses a kit for testing lipoprotein a(Lp(a)). The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is a buffer solution, and the reagent R2 is a mixture of lipoprotein a(Lp(a)) antibody sensitized polystyrene latex particles and the buffer solution. The kit has the advantages of simplicity and rapidness in detection, high sensitivity, good accuracy, high anti-interference capacity and low production cost; a method for detecting the lipoprotein a(Lp(a)) is latex enhanced immunoturbidimetry, with the adoption of the method, lipoprotein a(Lp(a)) can be detected more economically, more conveniently and more quickly, and the kit is suitable for automatic biochemical analyzers in most hospitals, and in particular, quick and quantitative detection can be realized during emergency treatment.