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Method for improving the accuracy of immunoturbidimetry

An immunoturbidimetric and accurate technology, applied in the field of medical immunodiagnosis, can solve the problems of low accuracy, latex aggregation and low sensitivity, and achieve the effect of low cost and enhanced accuracy

Pending Publication Date: 2019-03-29
海格德生物科技(深圳)有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a method for improving the accuracy of immunoturbidimetry, aiming at solving the phenomenon of latex aggregation that is prone to occur in the existing latex coupling process, the sensitivity is not high, and the result is easily affected by lipids. The influence of blood and hemolysis, low accuracy, etc.

Method used

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  • Method for improving the accuracy of immunoturbidimetry
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Embodiment Construction

[0028] The present invention provides a method for improving the accuracy of immunoturbidimetry. In order to make the purpose, technical solution and effect of the present invention clearer and clearer, the present invention will be further described in detail below. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0029] The invention provides a method for improving the accuracy of immunoturbidimetry, the specific steps are as follows:

[0030] Double magnetic beads with a particle size of 50-100 nm and 150-300 nm are used for coupling, and the mass ratio of the two magnetic beads is 2:3.

[0031] 1 Solution formula list

[0032] 1.1 The first detection solution

[0033] Raw material name

Prepare 1000mL dosage

Disodium hydrogen phosphate (Na 2 HPO 4 12H 2 O)

0.1-0.3g

Sodium dihydrogen phosphate (NaH 2 PO 4 2H 2 O)

2-4g

...

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PUM

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Abstract

The invention discloses a method for improving the accuracy of immunoturbidimetry. The method uses magnetic beads to couple antibodies, and uses a magnetic field to quickly separate, thereby shortening the coupling time, avoiding an agglomeration phenomenon caused by centrifugation, and improving the coupling efficiency. The method uses the magnetic beads with one or more particle sizes for coupling, and preferably uses magnetic beads with multi-particle size for coupling since the multi-particle size coupling has higher detection sensitivity and specificity relative to the single particle size. The immunoturbidimetric method is highly susceptible to hemolysis and fatty blood, resulting in inaccurate results. In the invention, the inaccurate results due to sample factors are avoided by applying a magnetic field for rapid separation, discarding the supernatant, adding the buffer to resuspend the complex, and then testing after a sample is reacted with the magnetic bead-antibody complex(after the antibody successfully captures the antigen).

Description

technical field [0001] The invention relates to the technical field of medical immunodiagnosis, in particular to a method for improving the accuracy of immunoturbidimetry. Background technique [0002] Immunoturbidimetric inhibition immunoassay (Turbidimetric inhibition immunoassay) is a dynamic assay method for antigen-antibody binding. The basic principle is: when the antigen and antibody react in a special dilution system and the ratio is appropriate (generally the antibody is excessive), the soluble immune complex Under the action of the polymerization accelerator (polyethylene glycol, etc.) in the dilution system, it precipitates from the liquid phase to form particles, which makes the reaction liquid appear turbid. When the antibody concentration is fixed, the amount of the formed immune complex increases with the increase of the amount of antigen in the test sample, and the turbidity of the reaction solution also increases accordingly. By measuring the turbidity of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/537G01N21/82
CPCG01N21/82G01N33/537
Inventor 卢岳军陈雪仇家舟钟运华陈晶晶
Owner 海格德生物科技(深圳)有限公司
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